PMID- 38409837
OWN - NLM
STAT- MEDLINE
DCOM- 20240228
LR  - 20240228
IS  - 1944-7930 (Electronic)
IS  - 1539-6509 (Linking)
VI  - 36
IP  - 181
DP  - 2024 Feb
TI  - SFRP5 Partially Inhibits the Proliferation and Migration of Airway Smooth Muscle 
      Cells in Children with Asthma by Regulating the Wnt/beta-Catenin Signaling Pathway.
PG  - 323-331
LID - 10.24976/Discov.Med.202436181.30 [doi]
AB  - BACKGROUND: Childhood asthma is a chronic inflammatory disease of the respiratory 
      tract characterized by bronchial inflammation, airway hyperresponsiveness, 
      airflow disorder, and obstruction. Secreted frizzled-related protein 5 (SFRP5) 
      may be associated with respiratory inflammatory diseases. This study investigated 
      the effect of SFRP5 on human airway smooth muscle cells (HASMCs) to provide new 
      ideas for treating asthma. METHODS: A total of 30 children with asthma and 30 
      children who had a physical examination at the same time were selected and 
      divided into asthma and healthy groups. Serum SFRP5 levels were determined by 
      enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase 
      chain reaction (RT-qPCR). Lipofectamine 2000 regent was used to transfect the 
      SFRP5 overexpression plasmid (pc-SFRP5) or corresponding negative control (pc-NC) 
      into HASMCs. HASMCs were treated with 10 mug/L platelet-derived growth factor-BB 
      (PDGF-BB), which is an inducer to mimic the asthma-like condition at the cellular 
      level of childhood asthma. HASMCs were divided into control, PDGF-BB (PDGF-BB 
      treatment), PDGF-BB+pc-NC (pc-NC transfection and PDGF-BB treatment), and 
      PDGF-BB+pc-SFRP5 (pc-SFRP5 transfection and PDGF-BB treatment) groups. Cell 
      proliferation was measured by 5-ethynyl-2'-deoxyuridine (EdU) and cell counting 
      kit-8 (CCK-8) assay. Cell migration was detected by Transwell assay. The protein 
      expression was detected by western blot. RESULTS: Serum SFRP5 expression in the 
      asthmatic group was decreased versus the healthy group (p < 0.0001). Induction of 
      PDGF-BB decreased SFRP5 expression in HASMCs (p < 0.01). SFRP5 expression in the 
      pc-SFRP5 group was increased (p < 0.01). The proliferation and migration of 
      HASMCs increased after PDGF-BB treatment (p < 0.001, p < 0.0001), indicating that 
      the asthma model was successfully inducted in vitro. Moreover, the expression of 
      beta-catenin, cellular-myelocytomatosis viral oncogene (c-Myc), and cyclinD1 
      proteins in HASMCs increased after PDGF-BB treatment (p < 0.0001). SFRP5 
      overexpression partly inhibited PDGF-BB-induced proliferation, migration, and 
      expressions of beta-catenin, c-Myc, and cyclinD proteins in HASMCs (p < 0.01, p < 
      0.001, p < 0.0001). CONCLUSIONS: Serum SFRP5 expression decreases in children 
      with asthma. SFRP5 overexpression partially inhibits PDGF-BB-induced HASMC 
      proliferation and migration by regulating the wingless-type mouse mammary tumor 
      virus (MMTV) integration site family (Wnt)/beta-catenin pathway.
FAU - Yuan, Yuyun
AU  - Yuan Y
AD  - Department of Pediatrics, Shanghai Baoshan Hospital of Integrated Traditional 
      Chinese and Western Medicine, 201999 Shanghai, China.
FAU - Zhu, Honghua
AU  - Zhu H
AD  - Department of Medical Imaging, Shanghai Seventh People's Hospital, 200137 
      Shanghai, China.
FAU - Huang, Sihong
AU  - Huang S
AD  - Department of Pediatrics, Shanghai Baoshan Hospital of Integrated Traditional 
      Chinese and Western Medicine, 201999 Shanghai, China.
FAU - Zhang, Yantao
AU  - Zhang Y
AD  - Department of Pediatrics, Shanghai Baoshan Hospital of Integrated Traditional 
      Chinese and Western Medicine, 201999 Shanghai, China.
FAU - Shen, Yiyun
AU  - Shen Y
AD  - Department of Pediatrics, Shanghai Baoshan Hospital of Integrated Traditional 
      Chinese and Western Medicine, 201999 Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Discov Med
JT  - Discovery medicine
JID - 101250006
RN  - 1B56C968OA (Becaplermin)
RN  - 0 (beta Catenin)
RN  - 0 (SFRP5 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
SB  - IM
MH  - Animals
MH  - Child
MH  - Mice
MH  - Humans
MH  - Becaplermin/metabolism/pharmacology
MH  - *beta Catenin/metabolism/pharmacology
MH  - Wnt Signaling Pathway/genetics
MH  - *Asthma/genetics/metabolism/pathology
MH  - Cell Proliferation/genetics
MH  - Lung/metabolism
MH  - Cell Movement
MH  - Myocytes, Smooth Muscle/metabolism/pathology
MH  - Cells, Cultured
MH  - Adaptor Proteins, Signal Transducing/metabolism
OTO - NOTNLM
OT  - HASMCs
OT  - SFRP5
OT  - Wnt/beta-catenin
OT  - children with asthma
OT  - migration
OT  - proliferation
EDAT- 2024/02/27 06:45
MHDA- 2024/02/28 06:45
CRDT- 2024/02/27 02:29
PHST- 2024/02/28 06:45 [medline]
PHST- 2024/02/27 06:45 [pubmed]
PHST- 2024/02/27 02:29 [entrez]
AID - 1708325300281-1780721949 [pii]
AID - 10.24976/Discov.Med.202436181.30 [doi]
PST - ppublish
SO  - Discov Med. 2024 Feb;36(181):323-331. doi: 10.24976/Discov.Med.202436181.30.