PMID- 38411606
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
DP  - 2024 Feb 27
TI  - Occurrence of Praxelis clematidea Witches'-Broom Disease Association with 16SrI 
      Group 'Candidatus Phytoplasma asteris' in Hainan Island of China.
LID - 10.1094/PDIS-01-24-0063-PDN [doi]
AB  - Praxelis clematidea is an invasive herbaceous plant belonging to Asteraceae 
      family. From August to November 2020, the plants showing severe witches'-broom 
      symptoms were found in farms and roadsides from Ding'an of Hainan Province, a 
      tropical island of China. The disease symptoms were suggestive of phytoplasma 
      infection. For pathogen detection, P. clematidea samples consisting of six 
      symptomatic and three asymptomatic plants were collected from the farms and 
      roadsites of Ding'an with 40 % incidence by conducting surveys and statistics. 
      Total nucleic acids were extracted using 0.10 g of fresh leaf tissues of the 
      plant through CTAB DNA extraction method. Conserved gene sequences of 16S rRNA 
      and secA genes from phytoplasma were amplified by direct PCR using primer pairs 
      of R16mF2/R16mR1 and secAfor1/secArev3, respectively. R16mF2/R16mR1 PCR amplicons 
      were obtained for all symptomatic samples but not from the symptomless plants. 
      The amplicons were purified and sequenced by Biotechnology (Shanghai) Co., Ltd. 
      (Guangzhou, China). Sequences of 16S rRNA gene (1323 bp) and secA (732 bp) were 
      obtained and all the gene sequences were identical, designated as PcWB (Praxelis 
      clematidea witches'-broom)-hnda. Representative sequencs were deposited in 
      Genbank with accession numbers of PP098736 (16S rDNA) and PP072216 (secA). 
      Nucleotide BLAST (Basic Local Alignment Search Tool) search based on 16S rRNA 
      gene sequences indicated that PcWB-hnda had 100% sequence identity (1323/1323) 
      with 'Candidatus Phytoplasma asteris'-related strains belonging to 16SrI group 
      like Waltheria indica virescence phytoplasma (MW353909) and Capsicum annuum 
      yellow crinkle phytoplasma (MT760793); had 99.62 % sequence identity (1321/1326) 
      with the phytoplasma strains of 16SrI group such as Oenothera phytoplasma 
      (M30790). RFLP (Restriction Fragment Length Polymorphism) pattern derived from 
      16Sr RNA gene sequences by iPhyClassifier showed identical (similarity 
      coefficient=1.00) to the reference pattern of 16SrI-B subgroup (GenBank accession 
      number: AP006628). The results obtained demonstrate that the phytoplasma strain 
      PcWB-hnda under study is a member of 16SrI-B subgroup. A BLAST search based on 
      secA gene sequences indicated that PcWB-hnda shares 100% sequence identity 
      (732/732 bp) with Pericampylus glaucus witches'-broom phytoplasma (MT875200), 99% 
      sequence identify (728/732 bp) with onion yellows phytoplasma OY-M(AP006628), and 
      99% sequence identify (729/732 bp) with rapeseed phyllody phytoplasma isolate 
      RP166 (CP055264), among other phytoplasma strains that belong to 16SrI group. 
      Previous studies demonstrated that P. clematidea can be infected by phytoplasmas 
      affiliate to the 16SrII group (GenBank accession number: KY568717 and EF061924) 
      in Hainan Island of China. To our knowledge, this is the first report of a 
      natural infection of P. clematidea by a group 16SrI phytoplasma in the Island of 
      China. 16SrI group can infect agronomic important species such as areca palm in 
      the island and P. clematidea can be a reservoir of 16SrI phytoplasmas. Therefore, 
      it is necessary to search of potential vectors of the pathogens, which would 
      contribute to epidemiological monitoring and prevention of the related diseases.
FAU - Yu, Shao-Shuai
AU  - Yu SS
AD  - Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, 
      Plant protection center, Coconut Research Institute, Wenqing Avenue 496, Wenchang 
      571339, Hainan province, People's Republic of China, Wenchang, China, 571339; 
      hzuyss@163.com.
FAU - Song, Chuan-Sheng
AU  - Song CS
AD  - Heze University, 226510, Department of Agriculture and Forestry, No.2269 Daxue 
      Road, Mudan District, Heze City, Shandong Province, China, Heze, Shandong, China, 
      274000; 244799824@qq.com.
FAU - Song, Wei-Wei
AU  - Song WW
AD  - Coconut Research Institute, Plant Protection, Wenchang, Hainan, China; 
      songweiwei426@sohu.com.
LA  - eng
PT  - Journal Article
DEP - 20240227
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
OTO - NOTNLM
OT  - Praxelis clematidea
OT  - molecular identification
OT  - phytoplasma
OT  - witches'-broom disease
OT  - 'Candidatus Phytoplasma asteris'
EDAT- 2024/02/27 12:47
MHDA- 2024/02/27 12:47
CRDT- 2024/02/27 10:53
PHST- 2024/02/27 12:47 [medline]
PHST- 2024/02/27 12:47 [pubmed]
PHST- 2024/02/27 10:53 [entrez]
AID - 10.1094/PDIS-01-24-0063-PDN [doi]
PST - aheadofprint
SO  - Plant Dis. 2024 Feb 27. doi: 10.1094/PDIS-01-24-0063-PDN.