PMID- 38412634
OWN - NLM
STAT- MEDLINE
DCOM- 20240318
LR  - 20240318
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 273
DP  - 2024 Mar 15
TI  - Paralytic shellfish toxins producing dinoflagellates cause dysbacteriosis in 
      scallop gut microbial biofilms.
PG  - 116146
LID - S0147-6513(24)00221-5 [pii]
LID - 10.1016/j.ecoenv.2024.116146 [doi]
AB  - Filter-feeding bivalves could accumulate paralytic shellfish toxins (PSTs) 
      produced by harmful dinoflagellates through diet. Despite that bivalves are 
      resistant to these neurotoxins due to possessing PST-resistant sodium channel, 
      exposure to PSTs-producing dinoflagellates impair bivalve survival. We 
      hypothesized that ingesting PSTs-producing dinoflagellates may influence the gut 
      microbiota, and then the health of bivalves. To test this idea, we compared the 
      gut microbiota of the scallop Patinopecten yessoensis, after feeding with 
      PST-producing or non-toxic dinoflagellates. Exposure to PSTs-producing 
      dinoflagellates resulted in a decline of gut microbial diversity and a 
      disturbance of community structure, accompanied by a significant increase in the 
      abundance and richness of pathogenic bacteria, represented by Vibrio. Moreover, 
      network analysis demonstrated extensive positive correlations between pathogenic 
      bacteria abundances and PSTs concentrations in the digestive glands of the 
      scallops. Furthermore, isolation of a dominant Vibrio strain and its genomic 
      analysis revealed a variety of virulence factors, including the tolC outer 
      membrane exporter, which were expressed in the gut microbiota. Finally, the 
      infection experiment demonstrated scallop mortality caused by the isolated Vibrio 
      strain; further, the pathogenicity of this Vibrio strain was attenuated by a 
      mutation in the tolC gene. Together, these findings demonstrated that the PSTs 
      may affect gut microbiota via direct and taxa-specific interactions with 
      opportunistic pathogens, which proliferate after transition from seawater to the 
      gut environment. The present study has revealed novel mechanisms towards 
      deciphering the puzzles in environmental disturbances-caused death of an 
      important aquaculture species.
CI  - Copyright (c) 2024. Published by Elsevier Inc.
FAU - Wei, Zhongcheng
AU  - Wei Z
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 
      266237, China.
FAU - Zhao, Liang
AU  - Zhao L
AD  - Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, China.
FAU - Wang, Shuaitao
AU  - Wang S
AD  - Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 
      China.
FAU - Chang, Lirong
AU  - Chang L
AD  - Weihai Changqing Ocean Science & Technology Co. Ltd, Rongcheng, China.
FAU - Shi, Jiaoxia
AU  - Shi J
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China.
FAU - Kong, Xiangfu
AU  - Kong X
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China.
FAU - Li, Moli
AU  - Li M
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China.
FAU - Lin, Jinshui
AU  - Lin J
AD  - Shaanxi Key Laboratory of Chinese Jujube, College of Life Sciences, Yanan 
      University, Yanan, China.
FAU - Zhang, Weipeng
AU  - Zhang W
AD  - Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, China.
FAU - Bao, Zhenmin
AU  - Bao Z
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 
      266237, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, 
      Sanya Oceanographic Institution, Ocean University of China, Sanya, China.
FAU - Ding, Wei
AU  - Ding W
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China. Electronic address: dingwei@ouc.edu.cn.
FAU - Hu, Xiaoli
AU  - Hu X
AD  - MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, 
      Qingdao, China; Laboratory for Marine Fisheries Science and Food Production 
      Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 
      266237, China. Electronic address: HXL707@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240226
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Toxins, Biological)
SB  - IM
MH  - Animals
MH  - *Dinoflagellida/chemistry
MH  - Dysbiosis
MH  - *Gastrointestinal Microbiome
MH  - *Toxins, Biological
MH  - *Pectinidae
MH  - *Bivalvia
MH  - Shellfish
MH  - *Shellfish Poisoning
OTO - NOTNLM
OT  - Genomics and metatranscriptomics
OT  - Gut microbiota
OT  - Harmful algal blooms, Paralytic shellfish toxin
OT  - Marine scallops
OT  - Vibrio
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper. Competing Interests The authors 
      declare no competing interests.
EDAT- 2024/02/28 00:43
MHDA- 2024/03/18 06:43
CRDT- 2024/02/27 18:06
PHST- 2023/11/16 00:00 [received]
PHST- 2024/02/05 00:00 [revised]
PHST- 2024/02/22 00:00 [accepted]
PHST- 2024/03/18 06:43 [medline]
PHST- 2024/02/28 00:43 [pubmed]
PHST- 2024/02/27 18:06 [entrez]
AID - S0147-6513(24)00221-5 [pii]
AID - 10.1016/j.ecoenv.2024.116146 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2024 Mar 15;273:116146. doi: 
      10.1016/j.ecoenv.2024.116146. Epub 2024 Feb 26.