PMID- 38412774
OWN - NLM
STAT- MEDLINE
DCOM- 20240315
LR  - 20240315
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 161
DP  - 2024 May
TI  - Redox biomarkers in saliva and nuclear abnormalities in jugal epithelial cells of 
      individuals with type 2 diabetes mellitus and periodontitis.
PG  - 105915
LID - S0003-9969(24)00036-0 [pii]
LID - 10.1016/j.archoralbio.2024.105915 [doi]
AB  - OBJECTIVE: To evaluate salivary redox biomarkers levels in individuals with 
      periodontitis and type 2 diabetes mellitus (T2DM) and correlate with periodontal 
      parameters and nuclear alterations in epithelial cells from jugal mucosa. DESIGN: 
      Sixty individuals were categorized into three groups: T2DM with periodontitis 
      (DM, n = 20), non-T2DM with periodontitis (PE, n = 20), and non-T2DM with 
      periodontal health (HC, n = 20). All participants underwent fasting blood glucose 
      and glycated hemoglobin measurements. After a periodontal examination, samples of 
      epithelial cells from the jugal mucosa and saliva were collected. DNA damage was 
      assessed by counting nuclear abnormalities using cytological analysis. Biomarkers 
      of oxidative stress were determined through biochemical methods. Significant 
      differences among groups were assessed using Kruskal-Wallis, Mann-Whitney, and 
      Chi-square tests at a 5% significance level. Data were analyzed using Spearman's 
      correlation coefficient, linear regression, and logistic regression. RESULTS: 
      Frequencies of nuclear abnormalities, as well as levels of reduced glutathione 
      and uric acid, were significantly higher in the DM group compared to the PE and 
      HC groups (p < 0.05). Fasting glucose, glycated hemoglobin, nuclear 
      abnormalities, reduced glutathione, and uric acid exhibited positive correlations 
      with periodontal parameters (p < 0.05). Furthermore, reduced glutathione was 
      associated with dental biofilm (OR = 1.027 [95% CI, 1.004-1.049]) and condensed 
      chromatin (OR = 0.415 [95% CI, 0.196-0.878]). CONCLUSIONS: Periodontitis and T2DM 
      are correlated with nuclear abnormalities, as well as salivary reduced 
      glutathione and uric acid levels. Moreover, a higher prevalence of teeth with 
      dental biofilm increases the likelihood of elevated levels of reduced glutathione 
      in saliva, while the presence of condensed chromatin decreases that likelihood.
CI  - Copyright (c) 2024 Elsevier Ltd. All rights reserved.
FAU - Lobao, Walder Jansen de Mello
AU  - Lobao WJM
AD  - Department of Dental Clinic, Division of Periodontics, School of Dentistry, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic 
      address: walderjansen@hotmail.com.
FAU - Zajdenverg, Lenita
AU  - Zajdenverg L
AD  - Department of Medical Clinic, Division of Nutrology, Faculty of Medicine, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Lopes, Gustavo Casimiro
AU  - Lopes GC
AD  - Physical Education and Sports Institute, Universidade do Estado do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Barros, Maria Cynesia Medeiros de
AU  - Barros MCM
AD  - Department of Dental Clinic, Division of Periodontics, School of Dentistry, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Sansone, Carmelo
AU  - Sansone C
AD  - Department of Dental Clinic, Division of Periodontics, School of Dentistry, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Silva-Boghossian, Carina M
AU  - Silva-Boghossian CM
AD  - Department of Dental Clinic, Division of Periodontics, School of Dentistry, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20240216
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Glycated Hemoglobin)
RN  - 268B43MJ25 (Uric Acid)
RN  - GAN16C9B8O (Glutathione)
RN  - 0 (Chromatin)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - *Diabetes Mellitus, Type 2/complications
MH  - Saliva/chemistry
MH  - Glycated Hemoglobin
MH  - Uric Acid/analysis
MH  - *Periodontitis/complications
MH  - Glutathione
MH  - Oxidation-Reduction
MH  - Chromatin
MH  - Biomarkers/analysis
MH  - *Chronic Periodontitis
OTO - NOTNLM
OT  - DNA damage
OT  - Oxidative stress
OT  - Periodontitis
OT  - Salivary biomarkers
OT  - Type 2 diabetes mellitus
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/28 00:43
MHDA- 2024/03/15 06:44
CRDT- 2024/02/27 18:12
PHST- 2023/10/31 00:00 [received]
PHST- 2024/02/14 00:00 [revised]
PHST- 2024/02/15 00:00 [accepted]
PHST- 2024/03/15 06:44 [medline]
PHST- 2024/02/28 00:43 [pubmed]
PHST- 2024/02/27 18:12 [entrez]
AID - S0003-9969(24)00036-0 [pii]
AID - 10.1016/j.archoralbio.2024.105915 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2024 May;161:105915. doi: 10.1016/j.archoralbio.2024.105915. Epub 
      2024 Feb 16.