PMID- 38414073
OWN - NLM
STAT- MEDLINE
DCOM- 20240229
LR  - 20240304
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 33
IP  - 2
DP  - 2024 Feb
TI  - Dermal thirdhand smoke exposure induced epidermal alterations in human 
      keratinocyte cells through oxidative damage and MMP-1 expression.
PG  - e15020
LID - 10.1111/exd.15020 [doi]
AB  - Thirdhand smoke (THS) is the residual cigarette smoke that settles on indoor 
      surface fabrics, dust and can accumulate in the environment. Therefore, it can be 
      a risk factor for individuals who have frequent dermal contact with 
      THS-contaminated surfaces. In the present study, it was aimed to elucidate the 
      toxicity of dermal THS exposure in HaCaT human keratinocytes. The THS was 
      extracted from terrycloth exposed to 3R4F research cigarette smoke in a closed 
      chamber and the adverse outcomes induced by THS were determined through 
      assessment of cytotoxicity tests (MTT and NRU), intracellular GSH level, total 
      SOD activity, matrix metalloproteinase-1 (MMP-1) and IL-6 levels. The wound 
      healing capacity of THS-exposed keratinocytes was evaluated via scratch assay. A 
      potent antioxidant isothiocyanate compound, sulforaphane (SFN), was used as a 
      negative control. THS was dose-dependently cytotoxic (12.5%-100%, v/v) to the 
      HaCaT cells through mitochondrial cell dysfunction (p < 0.01), which was 
      ameliorated by SFN (0.62 muM) pre-treatment. In parallel, THS exposure 
      significantly decreased the intracellular GSH deposits and T-SOD activity in 
      keratinocytes. Collagen degradation through elevated MMP-1 expression was 
      observed in THS-exposed cells in parallel with the delay of wound healing and 
      increased pro-inflammatory response in a dose-dependent manner (p < 0.05). The 
      findings are expected to raise awareness about THS as an environmental pollutant 
      for skin, particularly in the highest-ranked countries in cigarette consumption. 
      To conclude, these results might contribute to the studies on the importance of 
      dermal exposure to THS in the pathogenesis of epidermal alterations and the other 
      skin diseases.
CI  - (c) 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Reis, Rengin
AU  - Reis R
AUID- ORCID: 0000-0002-3484-2201
AD  - Department of Toxicology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar 
      University, Istanbul, Turkey.
FAU - Kolci, Kubra
AU  - Kolci K
AUID- ORCID: 0000-0003-4228-6564
AD  - Department of Toxicology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar 
      University, Istanbul, Turkey.
AD  - Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Istanbul, 
      Turkey.
FAU - Yedikardes, Eda Nil
AU  - Yedikardes EN
AUID- ORCID: 0009-0000-9184-0627
AD  - Department of Toxicology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar 
      University, Istanbul, Turkey.
FAU - Coskun, Goknil Pelin
AU  - Coskun GP
AUID- ORCID: 0000-0001-5168-3866
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Acibadem Mehmet Ali 
      Aydinlar University, Istanbul, Turkey.
FAU - Uzuner, Yasemin
AU  - Uzuner Y
AUID- ORCID: 0000-0001-6586-4158
AD  - Department of Pharmaceutical Technology, Faculty of Pharmacy, Acibadem Mehmet Ali 
      Aydinlar University, Istanbul, Turkey.
LA  - eng
GR  - The Scientific and Technological Research Council of Turkey (TUBITAK)/
PT  - Journal Article
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Tobacco Smoke Pollution)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Humans
MH  - *Tobacco Smoke Pollution/adverse effects
MH  - Matrix Metalloproteinase 1
MH  - Oxidative Stress
MH  - Keratinocytes
MH  - Superoxide Dismutase
MH  - Tobacco Products
OTO - NOTNLM
OT  - cytotoxicity
OT  - oxidative stress
OT  - sulforaphane
OT  - thirdhand smoke
OT  - wound healing
EDAT- 2024/02/28 06:43
MHDA- 2024/02/29 06:43
CRDT- 2024/02/28 00:01
PHST- 2023/11/30 00:00 [revised]
PHST- 2023/08/25 00:00 [received]
PHST- 2024/01/07 00:00 [accepted]
PHST- 2024/02/29 06:43 [medline]
PHST- 2024/02/28 06:43 [pubmed]
PHST- 2024/02/28 00:01 [entrez]
AID - 10.1111/exd.15020 [doi]
PST - ppublish
SO  - Exp Dermatol. 2024 Feb;33(2):e15020. doi: 10.1111/exd.15020.