PMID- 38415924
OWN - NLM
STAT- MEDLINE
DCOM- 20240229
LR  - 20240301
IS  - 2050-4527 (Electronic)
IS  - 2050-4527 (Linking)
VI  - 12
IP  - 2
DP  - 2024 Feb
TI  - Distinct cross talk of IL-17 & TGF-beta with the immature CD11c(+) TRAF6((-/-)) 
      -null myeloid dendritic cell-derived osteoclast precursor (mDDOCp) may engage 
      signaling toward an alternative pathway of osteoclastogenesis for arthritic bone 
      loss in vivo.
PG  - e1173
LID - 10.1002/iid3.1173 [doi]
LID - e1173
AB  - BACKGROUND: Dendritic cells (DCs), though borne heterogeneous, are the most 
      potent antigen-presenting cells, whose critical functions include triggering 
      antigen-specific naive T-cell responses and fine-tuning the innate versus 
      adaptive immunity at the osteo-immune and/or mucosal mesenchyme interface. We 
      previously reported that immature myeloid-CD11c(+) DCs/mDCs may act like 
      osteoclast (OC) precursors (OCp/mDDOCp) capable of developing into functional OCs 
      via an alternative pathway of inflammation-induced osteoclastogenesis; however, 
      what are their contribution and signaling interactions with key osteotropic 
      cytokines (i.e., interleukin-17 [IL-17] and transforming growth factor-beta [TGF-beta]) 
      to bearing such inflammatory bone loss in vivo remain unclear to date. METHODS: 
      Herein, we employed mature adult bone marrow-reconstituted C57BL/6 TRAF6((-/-)) 
      -null chimeras without the classical monocyte/macrophage (Mo/Mvarphi)-derived OCs to 
      address their potential contribution to OCp/mDDOCp-mediated osteoclastogenesis in 
      the chicken type-II-collagen (CC-II)-induced joint inflammation versus arthritic 
      bone loss and parallel associations with the double-positive CD11c(+) TRAP(+) 
      TRAF6-null((-/-))  DC-like OCs detected in vivo via the quantitative 
      dual-immunohistochemistry and digital histomorphometry for analyses. RESULTS: The 
      resulting findings revealed the unrecognized novel insight that (i) immature 
      myeloid-CD11c(+) TRAF6((-/-)) TRAP(+) DC-like OCs were involved, co-localized, 
      and strongly associated with joint inflammation and bone loss, independent of the 
      Mo/Mvarphi-derived classical OCs, in CC-II-immunized TRAF6((-/-)) -null chimeras, 
      and (ii) the osteotropic IL-17 may engage distinct crosstalk with CD11c(+) 
      mDCs/mDDOCp before developing the CD11c(+) TRAP(+) TRAF6((-/-)) OCs via a 
      TGF-beta-dependent interaction toward inflammation-induced arthritic bone loss in 
      vivo. CONCLUSION: These results confirm and substantiate the validity of 
      TRAF6((-/-)) -null chimeras to address the significance of immature mCD11c(+) 
      TRAP(+) DC-like OCs/mDDOCp subset for an alternative pathway of arthritic bone 
      loss in vivo. Such CD11c(+) mDCs/mDDOCp-associated osteoclastogenesis through the 
      step-wise twist-in-turns osteo-immune cross talks are thereby theme highlighted 
      to depict a summative re-visitation proposed.
CI  - (c) 2024 The Authors. Immunity, Inflammation and Disease published by John Wiley & 
      Sons Ltd.
FAU - Liu, Yen Chun G
AU  - Liu YCG
AD  - Department of Oral Hygiene, Center for Osteo-immunology & Biotechnology Research 
      (COBR), College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, 
      Taiwan.
AD  - School of Oral Hygiene & Nursing, and School of Dentistry, Kanagawa Dental 
      University (KDU), Yokosuka, Kanagawa, Japan.
FAU - Teng, Andy Yen-Tung
AU  - Teng AY
AUID- ORCID: 0000-0002-9977-190X
AD  - The Eastman Institute for Oral Health (EIOH), School of Medicine & Dentistry, 
      University of Rochester, Rochester, New York, USA.
AD  - Center for Osteo-immunology & Biotechnology Research (COBR), School of Dentistry, 
      College of Dental Medicine, Kaohsiung Medical University (KMU) and KMU-Hospital, 
      Kaohsiung, Taiwan.
LA  - eng
GR  - NHRI-EX101-9946SI/National Health Research Institute, Taiwan/
PT  - Journal Article
PL  - England
TA  - Immun Inflamm Dis
JT  - Immunity, inflammation and disease
JID - 101635460
RN  - 0 (Interleukin-17)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Humans
MH  - *Osteoclasts
MH  - *Osteogenesis
MH  - Interleukin-17
MH  - TNF Receptor-Associated Factor 6/genetics
MH  - Transforming Growth Factor beta
MH  - Dendritic Cells
MH  - Inflammation
PMC - PMC10851637
OTO - NOTNLM
OT  - IL-17 & TGF-beta
OT  - alternative pathway of osteoclastogenesis
OT  - arthritic bone loss
OT  - osteo-immune interactions
OT  - osteoclast versus CD11c+-myeloid dendritic cell derived osteoclast precursor 
      (mDDOCp)
COIS- The authors declare no conflicts of interest.
EDAT- 2024/02/28 12:43
MHDA- 2024/02/29 06:43
PMCR- 2024/02/08
CRDT- 2024/02/28 09:51
PHST- 2024/01/18 00:00 [revised]
PHST- 2023/07/30 00:00 [received]
PHST- 2024/01/18 00:00 [accepted]
PHST- 2024/02/29 06:43 [medline]
PHST- 2024/02/28 12:43 [pubmed]
PHST- 2024/02/28 09:51 [entrez]
PHST- 2024/02/08 00:00 [pmc-release]
AID - IID31173 [pii]
AID - 10.1002/iid3.1173 [doi]
PST - ppublish
SO  - Immun Inflamm Dis. 2024 Feb;12(2):e1173. doi: 10.1002/iid3.1173.