PMID- 38416753
OWN - NLM
STAT- MEDLINE
DCOM- 20240301
LR  - 20240301
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 19
IP  - 2
DP  - 2024
TI  - Comparison of hypoxia- and hyperoxia-induced alteration of epigene expression 
      pattern in lungs of Pleurodeles waltl and Mus musculus.
PG  - e0299661
LID - 10.1371/journal.pone.0299661 [doi]
LID - e0299661
AB  - Epigenetics is an emerging field of research because of its involvement in 
      susceptibility to diseases and aging. Hypoxia and hyperoxia are known to be 
      involved widely in various pathophysiologies. Here, we compared the differential 
      epigene expression pattern between Pleurodeles waltl and Mus musculus (commonly 
      known as Iberian ribbed newt and mouse, respectively) exposed to hypoxia and 
      hyperoxia. Adult healthy newts and mice were exposed to normobaric hypoxia (8% 
      O2) and hyperoxia (80% O2) for 2 hours. We collected the lungs and analyzed the 
      expression of hypoxia-inducible factor 1 alpha (Hif1alpha) and several key epigenes 
      from DNA methyltransferase (DNMT) family, histone deacetylase (HDAC) family, and 
      methyl-CpG binding domain (MBD) family. The exposure to hypoxia significantly 
      increased the mRNA levels of DNA methyltransferase 3 alpha (Dnmt3alpha), methyl-CpG 
      binding domain protein 2 (Mbd2), Mbd3, and histone deacetylase 2 (Hdac2) in lungs 
      of newts, but decreased the mRNA levels of DNA methyltransferase 1 (Dnmt1) and 
      Dnmt3alpha in lungs of mice. The exposure to hyperoxia did not significantly change 
      the expression of any gene in either newts or mice. The differential epigene 
      expression pattern in response to hypoxia between newts and mice may provide 
      novel insights into the prevention and treatment of disorders developed due to 
      hypoxia exposure.
CI  - Copyright: (c) 2024 Hasan et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Hasan, Md Mahmudul
AU  - Hasan MM
AUID- ORCID: 0000-0003-2489-6772
AD  - Department of Stem Cell Biology, Nagasaki University Graduate School of 
      Biomedical Sciences, Sakamoto, Nagasaki, Japan.
AD  - Department of Stem Cell Biology, Atomic Bomb Diseases Institute, Nagasaki 
      University, Sakamoto, Nagasaki, Japan.
FAU - Sekiya, Reiko
AU  - Sekiya R
AD  - Department of Stem Cell Biology, Nagasaki University Graduate School of 
      Biomedical Sciences, Sakamoto, Nagasaki, Japan.
AD  - Department of Stem Cell Biology, Atomic Bomb Diseases Institute, Nagasaki 
      University, Sakamoto, Nagasaki, Japan.
FAU - Zhang, Xu
AU  - Zhang X
AD  - Department of Stem Cell Biology, Nagasaki University Graduate School of 
      Biomedical Sciences, Sakamoto, Nagasaki, Japan.
AD  - Department of Stem Cell Biology, Atomic Bomb Diseases Institute, Nagasaki 
      University, Sakamoto, Nagasaki, Japan.
FAU - Yassouf, Mhd Yousuf
AU  - Yassouf MY
AD  - Department of Stem Cell Biology, Nagasaki University Graduate School of 
      Biomedical Sciences, Sakamoto, Nagasaki, Japan.
AD  - Department of Stem Cell Biology, Atomic Bomb Diseases Institute, Nagasaki 
      University, Sakamoto, Nagasaki, Japan.
FAU - Li, Tao-Sheng
AU  - Li TS
AUID- ORCID: 0000-0002-7653-8873
AD  - Department of Stem Cell Biology, Nagasaki University Graduate School of 
      Biomedical Sciences, Sakamoto, Nagasaki, Japan.
AD  - Department of Stem Cell Biology, Atomic Bomb Diseases Institute, Nagasaki 
      University, Sakamoto, Nagasaki, Japan.
LA  - eng
PT  - Journal Article
DEP - 20240228
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 2.1.1.- (Methyltransferases)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Pleurodeles/genetics
MH  - *Hyperoxia/genetics
MH  - Hypoxia/genetics
MH  - Salamandridae/genetics
MH  - Lung
MH  - RNA, Messenger/genetics
MH  - DNA
MH  - Methyltransferases
PMC - PMC10901355
COIS- The authors have declared that no competing interests exist.
EDAT- 2024/02/28 18:43
MHDA- 2024/03/01 06:44
PMCR- 2024/02/28
CRDT- 2024/02/28 13:34
PHST- 2023/10/13 00:00 [received]
PHST- 2024/02/12 00:00 [accepted]
PHST- 2024/03/01 06:44 [medline]
PHST- 2024/02/28 18:43 [pubmed]
PHST- 2024/02/28 13:34 [entrez]
PHST- 2024/02/28 00:00 [pmc-release]
AID - PONE-D-23-33168 [pii]
AID - 10.1371/journal.pone.0299661 [doi]
PST - epublish
SO  - PLoS One. 2024 Feb 28;19(2):e0299661. doi: 10.1371/journal.pone.0299661. 
      eCollection 2024.