PMID- 38425711
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240302
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 35
IP  - 1
DP  - 2024 Mar 12
TI  - MiR-449a antagonizes EMT through IL-6-mediated trans-signaling in laryngeal 
      squamous cancer.
PG  - 102140
LID - 10.1016/j.omtn.2024.102140 [doi]
LID - 102140
AB  - MicroRNAs (miRNAs) are involved in post-transcriptional gene expression 
      regulation and in mechanisms of cancer growth and metastases. In this light, 
      miRNAs could be promising therapeutic targets and biomarkers in clinical 
      practice. Therefore, we investigated if specific miRNAs and their target genes 
      contribute to laryngeal squamous cell carcinoma (LSCC) development. We found a 
      significant decrease of miR-449a in LSCC patients with nodal metastases (63.3%) 
      compared with patients without nodal involvement (44%). The AmpliSeq 
      Transcriptome of HNO-210 miR-449a-transfected cell lines allowed the 
      identification of IL6-R as a potential target. Moreover, the downregulation of 
      IL6-R and the phosphorylation reduction of the downstream signaling effectors, 
      suggested the inhibition of the IL-6 trans-signaling pathway. These biochemical 
      effects were paralleled by a significant inhibition of invasion and migration 
      in vitro and in vivo, supporting an involvement of epithelial-mesenchymal 
      transition. These findings indicate that miR-449a contributes to suppress the 
      metastasization of LSCC by the IL-6 trans-signaling block and affects sensitivity 
      to external stimuli that mimic pro-inflammatory conditions.
CI  - (c) 2024 The Author(s).
FAU - Cossu, Alessia Maria
AU  - Cossu AM
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Melisi, Federica
AU  - Melisi F
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Noviello, Teresa Maria Rosaria
AU  - Noviello TMR
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
AD  - Department of Electrical Engineering and Information Technology, University of 
      Naples "Federico II", Napoli, Italy.
FAU - Pasquale, Lucia Stefania
AU  - Pasquale LS
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Grisolia, Piera
AU  - Grisolia P
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Reale, Carla
AU  - Reale C
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Bocchetti, Marco
AU  - Bocchetti M
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Falco, Michela
AU  - Falco M
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Tammaro, Chiara
AU  - Tammaro C
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Accardo, Nunzio
AU  - Accardo N
AD  - Ear, Nose, and Throat Unit, AORN "Antonio Cardarelli", Naples, Italy.
FAU - Longo, Francesco
AU  - Longo F
AD  - Head and Neck Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, 
      "Fondazione G. Pascale", IRCCS, Naples, Italy.
FAU - Allosso, Salvatore
AU  - Allosso S
AD  - Department of Neurosciences, Reproductive and Odontostomatological Sciences, UOC 
      Federico II, 80121 Naples, Italy.
FAU - Mesolella, Massimo
AU  - Mesolella M
AD  - Department of Neurosciences, Reproductive and Odontostomatological Sciences, UOC 
      Federico II, 80121 Naples, Italy.
FAU - Addeo, Raffaele
AU  - Addeo R
AD  - Medical Oncology Unit, San Giovanni di Dio Hospital, 80027 Frattamaggiore, Italy.
FAU - Perri, Francesco
AU  - Perri F
AD  - Head and Neck Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, 
      "Fondazione G. Pascale", IRCCS, Naples, Italy.
FAU - Ottaiano, Alessandro
AU  - Ottaiano A
AD  - SSD Innovative Therapies for Abdominal Metastases, Abdominal Oncology, Istituto 
      Nazionale per lo Studio e la Cura dei Tumori, "Fondazione G. Pascale", IRCCS, 
      Naples, Italy.
FAU - Ricciardiello, Filippo
AU  - Ricciardiello F
AD  - Ear, Nose, and Throat Unit, AORN "Antonio Cardarelli", Naples, Italy.
FAU - Amler, Evzen
AU  - Amler E
AD  - UCEEB, Czech Technical University, Trinecka 1024, 273 43 Bustehrad, Czech.
FAU - Ambrosino, Concetta
AU  - Ambrosino C
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
AD  - Department of Science and Technology, University of Sannio, 82100 Benevento, 
      Italy.
FAU - Misso, Gabriella
AU  - Misso G
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Ceccarelli, Michele
AU  - Ceccarelli M
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
AD  - Department of Electrical Engineering and Information Technology, University of 
      Naples "Federico II", Napoli, Italy.
FAU - Caraglia, Michele
AU  - Caraglia M
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
FAU - Scrima, Marianna
AU  - Scrima M
AD  - Biogem Scarl, Institute of Genetic Research, 83031 Ariano Irpino, Italy.
LA  - eng
PT  - Journal Article
DEP - 20240206
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC10901858
OTO - NOTNLM
OT  - IL-6 trans-signaling
OT  - LSCC
OT  - MT: non-coding RNAs
OT  - gene expression
OT  - metastases miR-449a
OT  - microRNAs
COIS- The all authors declare that they have no conflicts of interests.
EDAT- 2024/03/01 06:44
MHDA- 2024/03/01 06:45
PMCR- 2024/02/06
CRDT- 2024/03/01 03:51
PHST- 2023/08/30 00:00 [received]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/03/01 06:45 [medline]
PHST- 2024/03/01 06:44 [pubmed]
PHST- 2024/03/01 03:51 [entrez]
PHST- 2024/02/06 00:00 [pmc-release]
AID - S2162-2531(24)00027-1 [pii]
AID - 102140 [pii]
AID - 10.1016/j.omtn.2024.102140 [doi]
PST - epublish
SO  - Mol Ther Nucleic Acids. 2024 Feb 6;35(1):102140. doi: 10.1016/j.omtn.2024.102140. 
      eCollection 2024 Mar 12.