PMID- 38426134
OWN - NLM
STAT- MEDLINE
DCOM- 20240304
LR  - 20240304
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 12
DP  - 2024
TI  - Multiscale transport and 4D time-lapse imaging in precision-cut liver slices 
      (PCLS).
PG  - e16994
LID - 10.7717/peerj.16994 [doi]
LID - e16994
AB  - BACKGROUND: Monitoring cellular processes across different levels of complexity, 
      from the cellular to the tissue scale, is important for understanding tissue 
      structure and function. However, it is challenging to monitor and estimate these 
      structural and dynamic interactions within three-dimensional (3D) tissue models. 
      OBJECTIVE: The aim of this study was to design a method for imaging, tracking, 
      and quantifying 3D changes in cell morphology (shape and size) within liver 
      tissue, specifically a precision-cut liver slice (PCLS). A PCLS is a 3D model of 
      the liver that allows the study of the structure and function of liver cells in 
      their native microenvironment. METHODS: Here, we present a method for imaging 
      liver tissue during anisosmotic exposure in a multispectral four-dimensional 
      manner. Three metrics of tissue morphology were measured to quantify the effects 
      of osmotic stress on liver tissue. We estimated the changes in the volume of 
      whole precision cut liver slices, quantified the changes in nuclei position, and 
      calculated the changes in volumetric responses of tissue-embedded cells. RESULTS: 
      During equilibration with cell-membrane-permeating and non-permeating solutes, 
      the whole tissue experiences shrinkage and expansion. As nuclei showed a change 
      in position and directional displacement under osmotic stress, we demonstrate 
      that nuclei could be used as a probe to measure local osmotic and mechanical 
      stress. Moreover, we demonstrate that cells change their volume within tissue 
      slices as a result of osmotic perturbation and that this change in volume is 
      dependent on the position of the cell within the tissue and the duration of the 
      exposure. CONCLUSION: The results of this study have implications for a better 
      understanding of multiscale transport, mechanobiology, and triggered biological 
      responses within complex biological structures.
CI  - (c)2024 Azam and Benson.
FAU - Azam, Iqra
AU  - Azam I
AD  - Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, 
      Canada.
FAU - Benson, James D
AU  - Benson JD
AD  - Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, 
      Canada.
LA  - eng
PT  - Journal Article
DEP - 20240226
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Wistar
MH  - Time-Lapse Imaging
MH  - *Liver/diagnostic imaging
MH  - Osmosis
MH  - Osmotic Pressure
PMC - PMC10903333
OTO - NOTNLM
OT  - Cryopreservation
OT  - Hepatocyte
OT  - Image analysis
OT  - Mechanobiology
OT  - Multiscale modeling
OT  - Nuclear displacement
OT  - Osmotic stress
OT  - Permeating solute
OT  - Precision cut liver slices (PCLS)
OT  - Three-dimensional imaging
COIS- The authors declare there are no competing interests.
EDAT- 2024/03/01 06:44
MHDA- 2024/03/04 06:45
PMCR- 2024/02/26
CRDT- 2024/03/01 03:58
PHST- 2023/09/21 00:00 [received]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/03/04 06:45 [medline]
PHST- 2024/03/01 06:44 [pubmed]
PHST- 2024/03/01 03:58 [entrez]
PHST- 2024/02/26 00:00 [pmc-release]
AID - 16994 [pii]
AID - 10.7717/peerj.16994 [doi]
PST - epublish
SO  - PeerJ. 2024 Feb 26;12:e16994. doi: 10.7717/peerj.16994. eCollection 2024.