PMID- 38426543
OWN - NLM
STAT- MEDLINE
DCOM- 20240304
LR  - 20240307
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 53
IP  - 4
DP  - 2024 Apr
TI  - Identification of novel molecules and pathways associated with fascin 
      actin‑bundling protein 1 in laryngeal squamous cell carcinoma through 
      comprehensive transcriptome analysis.
LID - 39 [pii]
LID - 10.3892/ijmm.2024.5363 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor with a poor 
      prognosis. Fascin actin‑bundling protein 1 (FSCN1) has been reported to play a 
      crucial role in the development and progression of LSCC; however, the underlying 
      molecular mechanisms remain unknown. Herein, a whole transcriptome microarray 
      analysis was performed to screen for differentially expressed genes (DEGs) in 
      cells in which FSCN1 was knocked down. A total of 462 up and 601 downregulated 
      mRNA transcripts were identified. Functional annotation analysis revealed that 
      these DEGs were involved in multiple biological functions, such as 
      transcriptional regulation, response to radiation, focal adhesion, extracellular 
      matrix‑receptor interaction, steroid biosynthesis and others. Through 
      co‑expression and protein‑protein interaction analysis, FSCN1 was linked to novel 
      functions, including defense response to virus and steroid biosynthesis. 
      Furthermore, crosstalk analysis with FSCN1‑interacting proteins revealed seven 
      DEGs, identified as FSCN1‑interacting partners, in LSCC cells, three of which 
      were selected for further validation. Co‑immunoprecipitation validation confirmed 
      that FSCN1 interacted with prostaglandin reductase 1 and 24‑dehydrocholesterol 
      reductase (DHCR24). Of note, DHCR24 is a key enzyme involved in cholesterol 
      biosynthesis, and its overexpression promotes the proliferation and migration of 
      LSCC cells. These findings suggest that DHCR24 is a novel molecule associated 
      with FSCN1 in LSCC, and that the FSCN1‑DHCR24 interaction may promote LSCC 
      progression by regulating cholesterol metabolism‑related signaling pathways.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Hao, Wenjing
AU  - Hao W
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Wang, Xinfang
AU  - Wang X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Zhang, Yuliang
AU  - Zhang Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - He, Long
AU  - He L
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Xue, Xuting
AU  - Xue X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Yang, Jiao
AU  - Yang J
AD  - Department of Anatomy, the Basic Medical School of Shanxi Medical University, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital 
      of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20240301
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 146808-54-0 (fascin)
RN  - 0 (Actins)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.- (Oxidoreductases)
RN  - 0 (Steroids)
RN  - 0 (MicroRNAs)
RN  - 0 (FSCN1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Microfilament Proteins)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Actins/metabolism
MH  - *Laryngeal Neoplasms/metabolism
MH  - *Carcinoma, Squamous Cell/metabolism
MH  - Gene Expression Profiling
MH  - *Head and Neck Neoplasms/genetics
MH  - Cholesterol
MH  - Oxidoreductases/genetics/metabolism
MH  - Steroids
MH  - Gene Expression Regulation, Neoplastic
MH  - *MicroRNAs/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Carrier Proteins
MH  - *Microfilament Proteins
PMC - PMC10914310
OTO - NOTNLM
OT  - 24‑dehydrocholesterol reductase
OT  - cholesterol metabolism
OT  - co‑expression
OT  - fascin
OT  - laryngeal squamous cell carcinoma
OT  - protein‑protein interaction
COIS- The authors declare that they have no competing interests.
EDAT- 2024/03/01 12:43
MHDA- 2024/03/04 06:50
PMCR- 2024/02/28
CRDT- 2024/03/01 08:09
PHST- 2023/12/01 00:00 [received]
PHST- 2024/02/14 00:00 [accepted]
PHST- 2024/03/04 06:50 [medline]
PHST- 2024/03/01 12:43 [pubmed]
PHST- 2024/03/01 08:09 [entrez]
PHST- 2024/02/28 00:00 [pmc-release]
AID - 39 [pii]
AID - ijmm-53-04-05363 [pii]
AID - 10.3892/ijmm.2024.5363 [doi]
PST - ppublish
SO  - Int J Mol Med. 2024 Apr;53(4):39. doi: 10.3892/ijmm.2024.5363. Epub 2024 Mar 1.