PMID- 38426941
OWN - NLM
STAT- MEDLINE
DCOM- 20240403
LR  - 20240403
IS  - 1532-2378 (Electronic)
IS  - 1049-5398 (Linking)
VI  - 35
IP  - 1
DP  - 2024 Nov
TI  - Multi-tissue gene expression profiling of cows with a genetic predisposition for 
      low and high milk urea levels.
PG  - 2322542
LID - 10.1080/10495398.2024.2322542 [doi]
AB  - Milk urea (MU) concentration is proposed as an indicator trait for breeding 
      toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 
      German Holstein cows based on MU breeding values, with 10 cows each having low 
      (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized 
      these cows to unravel molecular pathways governing post-absorptive body N pools 
      focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic 
      urea formation and mammary gland N excretion. While we observed minor adjustments 
      in cellular energy metabolism in different tissues associated with different MU 
      levels, no transcriptional differences in liver ammonia detoxification were 
      detected, despite significant differences in MU between the groups. Differential 
      expression of AQP3 and SLC38A2 in the kidney provides evidence for higher urea 
      concentration in the collecting duct of LMU cows than HMU cows. The mammary gland 
      exhibited the most significant differences, particularly in tricarboxylic acid 
      (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. 
      These findings suggest that selecting for lower MU could lead to altered urinary 
      urea (UU) handling and changes in milk protein synthesis. However, given the 
      genetic variability in N metabolism components, the long-term effectiveness of 
      MU-based selection in reducing N emissions remains uncertain.
FAU - Reyer, Henry
AU  - Reyer H
AD  - Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), 
      Dummerstorf, Germany.
FAU - Honerlagen, Hanne
AU  - Honerlagen H
AD  - Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), 
      Dummerstorf, Germany.
AD  - Animal Breeding and Genomics, Wageningen University and Research, Wageningen, The 
      Netherlands.
FAU - Oster, Michael
AU  - Oster M
AD  - Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), 
      Dummerstorf, Germany.
FAU - Ponsuksili, Siriluck
AU  - Ponsuksili S
AD  - Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), 
      Dummerstorf, Germany.
FAU - Kuhla, Bjorn
AU  - Kuhla B
AD  - Institute of Nutritional Physiology 'Oskar Kellner', Research Institute for Farm 
      Animal Biology (FBN), Dummerstorf, Germany.
FAU - Wimmers, Klaus
AU  - Wimmers K
AD  - Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), 
      Dummerstorf, Germany.
AD  - Faculty of Agriculture and Environmental Sciences, Professorship of Animal 
      Breeding and Genetics, University of Rostock, Rostock, Germany.
LA  - eng
PT  - Journal Article
DEP - 20240301
PL  - England
TA  - Anim Biotechnol
JT  - Animal biotechnology
JID - 9011409
RN  - 0 (Milk Proteins)
RN  - 8W8T17847W (Urea)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Female
MH  - Cattle/genetics
MH  - Animals
MH  - *Lactation
MH  - *Milk/chemistry
MH  - Milk Proteins
MH  - Urea/analysis/metabolism
MH  - RNA-Seq
MH  - Nitrogen/metabolism
MH  - Diet/veterinary
OTO - NOTNLM
OT  - RNAseq
OT  - kidney
OT  - liver
OT  - mammary gland
OT  - nitrogen emissions
EDAT- 2024/03/01 12:43
MHDA- 2024/04/03 06:44
CRDT- 2024/03/01 10:33
PHST- 2024/04/03 06:44 [medline]
PHST- 2024/03/01 12:43 [pubmed]
PHST- 2024/03/01 10:33 [entrez]
AID - 10.1080/10495398.2024.2322542 [doi]
PST - ppublish
SO  - Anim Biotechnol. 2024 Nov;35(1):2322542. doi: 10.1080/10495398.2024.2322542. Epub 
      2024 Mar 1.