PMID- 38428103 OWN - NLM STAT- MEDLINE DCOM- 20240325 LR - 20240325 IS - 0306-4565 (Print) IS - 0306-4565 (Linking) VI - 120 DP - 2024 Feb TI - Heat stress enhances the occurrence of erythromycin resistance of Enterococcus isolates in mice feces. PG - 103786 LID - S0306-4565(24)00004-4 [pii] LID - 10.1016/j.jtherbio.2024.103786 [doi] AB - Heat stress is a common environmental factor in livestock breeding that has been shown to impact the development of antibiotic resistance within the gut microbiota of both human and animals. However, studies investigating the effect of temperature on antibiotic resistance in Enterococcus isolates remain limited. In this study, specific pathogen free (SPF) mice were divided into a control group maintained at normal temperature and an experimental group subjected to daily 1-h heat stress at 38 degrees C, respectively. Gene expression analysis was conducted to evaluate the activation of heat shock responsive genes in the liver of mice. Additionally, the antibiotic-resistant profile and antibiotic resistant genes (ARGs) in fecal samples from mice were analyzed. The results showed an upregulation of heat-inducible proteins HSP27, HSP70 and HSP90 following heat stress exposure, indicating successful induction of cellular stress within the mice. Furthermore, heat stress resulted in an increase in the proportion of erythromycin-resistant Enterococcus isolates, escalating from 0 % to 0.23 % over a 30-day duration of heat stress. The resistance of Enterococcus isolates to erythromycin also had a 128-fold increase in minimum inhibitory concentration (MIC) within the heated-stressed group compared to the control group. Additionally, a 2 approximately 8-fold rise in chloramphenicol MIC was observed among these erythromycin-resistant Enterococcus isolates. The acquisition of ermB genes was predominantly responsible for mediating the erythromycin resistance in these Enterococcus isolates. Moreover, the abundance of macrolide, lincosamide and streptogramin (MLS) resistant-related genes in the fecal samples from the heat-stressed group exhibited a significant elevation compared to the control group, primarily driven by changes in bacterial community composition, especially Enterococcaceae and Planococcaceae, and the transfer of mobile genetic elements (MGEs), particularly insertion elements. Collectively, these results highlight the role of environmental heat stress in promoting antibiotic resistance in Enterococcus isolates and partly explain the increasing prevalence of erythromycin-resistant Enterococcus isolates observed among animals in recent years. CI - Copyright (c) 2024 Elsevier Ltd. All rights reserved. FAU - Yi, Lingxian AU - Yi L AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Xu, Rui AU - Xu R AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Yuan, Xiaowu AU - Yuan X AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Ren, Zining AU - Ren Z AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Song, Huihui AU - Song H AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Lai, Huamin AU - Lai H AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Sun, Zhihua AU - Sun Z AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Deng, Hui AU - Deng H AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Yang, Bo AU - Yang B AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Yu, Daojin AU - Yu D AD - Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: DaojinYu@outlook.com. LA - eng PT - Journal Article DEP - 20240128 PL - England TA - J Therm Biol JT - Journal of thermal biology JID - 7600115 RN - 63937KV33D (Erythromycin) RN - 0 (Anti-Bacterial Agents) SB - IM MH - Humans MH - Animals MH - Mice MH - *Erythromycin/pharmacology MH - *Enterococcus/genetics MH - Anti-Bacterial Agents/pharmacology MH - Feces MH - Heat-Shock Response OTO - NOTNLM OT - Antibiotic resistance OT - Enterococcus isolates OT - Heat stress OT - Intestinal flora COIS- Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest EDAT- 2024/03/02 10:43 MHDA- 2024/03/25 06:42 CRDT- 2024/03/01 18:02 PHST- 2023/07/13 00:00 [received] PHST- 2024/01/09 00:00 [revised] PHST- 2024/01/16 00:00 [accepted] PHST- 2024/03/25 06:42 [medline] PHST- 2024/03/02 10:43 [pubmed] PHST- 2024/03/01 18:02 [entrez] AID - S0306-4565(24)00004-4 [pii] AID - 10.1016/j.jtherbio.2024.103786 [doi] PST - ppublish SO - J Therm Biol. 2024 Feb;120:103786. doi: 10.1016/j.jtherbio.2024.103786. Epub 2024 Jan 28.