PMID- 38436394
OWN - NLM
STAT- MEDLINE
DCOM- 20240327
LR  - 20240327
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 24
IP  - 7
DP  - 2024 Mar 26
TI  - Tonicity-induced cargo loading into extracellular vesicles.
PG  - 2069-2079
LID - 10.1039/d3lc00830d [doi]
AB  - The current challenge in using extracellular vesicles (EVs) as drug delivery 
      vehicles is to precisely control their membrane permeability, specifically in the 
      ability to switch between permeable and impermeable states without compromising 
      their integrity and functionality. Here, we introduce a rapid, efficient, and 
      gentle loading method for EVs based on tonicity control (TC) using a 
      lab-on-a-disc platform. In this technique, a hypotonic solution was used for 
      temporarily permeabilizing a membrane ("on" state), allowing the influx of 
      molecules into EVs. The subsequent isotonic washing led to an impermeable 
      membrane ("off" state). This loading cycle enables the loading of different 
      cargos into EVs, such as doxorubicin hydrochloride (Dox), ssDNA, and miRNA. The 
      TC approach was shown to be more effective than traditional methods such as 
      sonication or extrusion, with loading yields that were 4.3-fold and 7.2-fold 
      greater, respectively. Finally, the intracellular assessments of miRNA-497-loaded 
      EVs and doxorubicin-loaded EVs confirmed the superior performance of TC-prepared 
      formulations and demonstrated the impact of encapsulation heterogeneity on the 
      therapeutic outcome, signifying potential opportunities for developing novel 
      exosome-based therapeutic systems for clinical applications.
FAU - Lee, Chaeeun
AU  - Lee C
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Kumar, Sumit
AU  - Kumar S
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Park, Juhee
AU  - Park J
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Choi, Yongjun
AU  - Choi Y
AUID- ORCID: 0000-0002-3814-3643
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Clarissa, Elizabeth Maria
AU  - Clarissa EM
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Cho, Yoon-Kyoung
AU  - Cho YK
AUID- ORCID: 0000-0001-6423-1834
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
LA  - eng
PT  - Journal Article
DEP - 20240326
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (MicroRNAs)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - *Extracellular Vesicles
MH  - *MicroRNAs
MH  - *Exosomes
MH  - Cell Communication
MH  - Doxorubicin/pharmacology
MH  - Drug Delivery Systems/methods
EDAT- 2024/03/04 12:45
MHDA- 2024/03/27 06:43
CRDT- 2024/03/04 09:55
PHST- 2024/03/27 06:43 [medline]
PHST- 2024/03/04 12:45 [pubmed]
PHST- 2024/03/04 09:55 [entrez]
AID - 10.1039/d3lc00830d [doi]
PST - epublish
SO  - Lab Chip. 2024 Mar 26;24(7):2069-2079. doi: 10.1039/d3lc00830d.