PMID- 38438383
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240308
IS  - 2731-6068 (Electronic)
IS  - 2731-6068 (Linking)
VI  - 10
IP  - 1
DP  - 2024 Mar 4
TI  - Effects of ageing and frailty on circulating monocyte and dendritic cell subsets.
PG  - 17
LID - 10.1038/s41514-024-00144-6 [doi]
LID - 17
AB  - Ageing is associated with dysregulated immune responses, resulting in impaired 
      resilience against infections and low-grade inflammation known as inflammageing. 
      Frailty is a measurable condition in older adults characterized by decreased 
      health and physical impairment. Dendritic cells (DCs) and monocytes play a 
      crucial role in initiating and steering immune responses. To assess whether their 
      frequencies and phenotypes in the blood are affected by ageing or frailty, we 
      performed a flow cytometry study on monocyte and DC subsets in an immune ageing 
      cohort. We included (n = 15 in each group) healthy young controls (HYC, median 
      age 29 years), healthy older controls (HOC, 73 years) and Frail older controls 
      (76 years). Monocyte subsets (classical, intermediate, non-classical) were 
      identified by CD14 and CD16 expression, and DC subsets (conventional (c)DC1, 
      cDC2, plasmacytoid (p)DC) by CD11c, CD1c, CD141 and CD303 expression. All subsets 
      were checked for TLR2, TLR4, HLA-DR, CD86, PDL1, CCR7 and CD40 expression. We 
      observed a lower proportion of pDCs in HOC compared to HYC. Additionally, we 
      found higher expression of activation markers on classical and intermediate 
      monocytes and on cDC2 in HOC compared to HYC. Frail participants had a higher 
      expression of CD40 on classical and non-classical monocytes compared to the HOC 
      group. We document a substantial effect of ageing on monocytes and DCs. Reduced 
      pDCs in older people may underlie their impaired ability to counter viral 
      infections, whereas enhanced expression of activation markers could indicate a 
      state of inflammageing. Future studies could elucidate the functional 
      consequences of CD40 upregulation with frailty.
CI  - (c) 2024. The Author(s).
FAU - Reitsema, Rosanne D
AU  - Reitsema RD
AUID- ORCID: 0000-0002-4250-0930
AD  - Department of Rheumatology and Clinical Immunology, University Medical Center 
      Groningen, Groningen, The Netherlands.
AD  - School of Medical Sciences, Faculty of Medicine and Health, Orebro University, 
      Orebro, Sweden.
FAU - Kumawat, Ashok K
AU  - Kumawat AK
AUID- ORCID: 0000-0002-2244-9816
AD  - School of Medical Sciences, Faculty of Medicine and Health, Orebro University, 
      Orebro, Sweden.
FAU - Hesselink, Bernd-Cornel
AU  - Hesselink BC
AD  - Department of Rheumatology and Clinical Immunology, University Medical Center 
      Groningen, Groningen, The Netherlands.
FAU - van Baarle, Debbie
AU  - van Baarle D
AD  - Department of Medical Microbiology and Infection Prevention, University Medical 
      Center Groningen, Groningen, The Netherlands.
FAU - van Sleen, Yannick
AU  - van Sleen Y
AUID- ORCID: 0000-0002-7382-8322
AD  - Department of Rheumatology and Clinical Immunology, University Medical Center 
      Groningen, Groningen, The Netherlands. y.van.sleen@umcg.nl.
LA  - eng
PT  - Journal Article
DEP - 20240304
PL  - England
TA  - NPJ Aging
JT  - npj aging
JID - 9918402285106676
PMC - PMC10912203
COIS- The authors declare no competing interests.
EDAT- 2024/03/05 00:45
MHDA- 2024/03/05 00:46
PMCR- 2024/03/04
CRDT- 2024/03/04 23:16
PHST- 2023/10/23 00:00 [received]
PHST- 2024/02/14 00:00 [accepted]
PHST- 2024/03/05 00:46 [medline]
PHST- 2024/03/05 00:45 [pubmed]
PHST- 2024/03/04 23:16 [entrez]
PHST- 2024/03/04 00:00 [pmc-release]
AID - 10.1038/s41514-024-00144-6 [pii]
AID - 144 [pii]
AID - 10.1038/s41514-024-00144-6 [doi]
PST - epublish
SO  - NPJ Aging. 2024 Mar 4;10(1):17. doi: 10.1038/s41514-024-00144-6.