PMID- 38441294
OWN - NLM
STAT- MEDLINE
DCOM- 20240306
LR  - 20240306
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 99
IP  - 3
DP  - 2024 Mar
TI  - BCG infection dose guides dendritic cell migration and T cell priming in the 
      draining lymph node.
PG  - e13342
LID - 10.1111/sji.13342 [doi]
AB  - In contrast to delayed-type hypersensitivity (DTH) and other hallmark reactions 
      of cell-mediated immunity that correlate with vaccine-mediated protection against 
      Mycobacterium tuberculosis, the contribution of vaccine dose on responses that 
      emerge early after infection in the skin with Bacille Calmette-Guerin (BCG) is 
      not well understood. We used a mouse model of BCG skin infection to study the 
      effect of BCG dose on the relocation of skin Dendritic cells (DCs) to draining 
      lymph node (DLN). Mycobacterium antigen 85B-specific CD4(+) P25 T cell-receptor 
      transgenic (P25 TCRTg) cells were used to probe priming to BCG in DLN. DC 
      migration and T cell priming were studied across BCG inocula that varied up to 
      100-fold (10(4) to 10(6) Colony-forming units-CFUs). In line with earlier results 
      in guinea pigs, DTH reaction in our model correlated with BCG dose. Importantly, 
      priming of P25 TCRTg cells in DLN also escalated in a dose-dependent manner, 
      peaking at day 6 after infection. Similar dose-escalation effects were seen for 
      DC migration from infected skin and the accompanying transport of BCG to the DLN. 
      BCG-triggered upregulation of co-stimulatory molecules on migratory DCs was 
      restricted to the first 24 hour after infection and was independent of BCG dose 
      over a 10-fold range (10(5) to 10(6) CFUs). The dose seemed to be a determinant 
      of the number of total skin DCs that move to the DLN. In summary, our results 
      support the use of higher BCG doses to detect robust DC migration and T cell 
      priming.
CI  - (c) 2023 The Authors. Scandinavian Journal of Immunology published by John Wiley & 
      Sons Ltd on behalf of The Scandinavian Foundation for Immunology.
FAU - Krmeska, Veronika
AU  - Krmeska V
AUID- ORCID: 0000-0001-8094-6266
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Shen, Lei
AU  - Shen L
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Nylen, Susanne
AU  - Nylen S
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Wowk, Pryscilla Fanini
AU  - Wowk PF
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      Stockholm, Sweden.
AD  - Instituto Carlos Chagas, Fundacao Oswaldo Cruz (ICC/Fiocruz-PR), Curitiba, 
      Brazil.
FAU - Rothfuchs, Antonio Gigliotti
AU  - Rothfuchs AG
AUID- ORCID: 0000-0001-6001-7240
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      Stockholm, Sweden.
LA  - eng
GR  - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
GR  - Instituto Carlos Chagas, Fundacao Oswaldo Cruz/
GR  - Karolinska Institutet/
GR  - Vetenskapsradet/
PT  - Journal Article
DEP - 20231121
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Guinea Pigs
MH  - *T-Lymphocytes
MH  - *BCG Vaccine
MH  - Immunity, Cellular
MH  - Langerhans Cells
MH  - Lymph Nodes
OTO - NOTNLM
OT  - BCG
OT  - dose
OT  - lymph node
EDAT- 2024/03/05 12:44
MHDA- 2024/03/06 06:43
CRDT- 2024/03/05 10:03
PHST- 2023/10/11 00:00 [revised]
PHST- 2023/05/26 00:00 [received]
PHST- 2023/11/09 00:00 [accepted]
PHST- 2024/03/06 06:43 [medline]
PHST- 2024/03/05 12:44 [pubmed]
PHST- 2024/03/05 10:03 [entrez]
AID - 10.1111/sji.13342 [doi]
PST - ppublish
SO  - Scand J Immunol. 2024 Mar;99(3):e13342. doi: 10.1111/sji.13342. Epub 2023 Nov 21.