PMID- 38441377
OWN - NLM
STAT- MEDLINE
DCOM- 20240306
LR  - 20240306
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 98
IP  - 4
DP  - 2023 Oct
TI  - MCP-1/CCR2 axis is involved in the regulation of gammadeltaT cells in lupus nephritis.
PG  - e13305
LID - 10.1111/sji.13305 [doi]
AB  - gammadeltaT cells are important innate immune cells that are involved in the occurrence 
      and development of autoimmune diseases such as systemic lupus erythematosus 
      (SLE). Lupus nephritis (LN) is a serious complication of SLE, characterized by 
      the accumulation of immune cells (including gammadeltaT cells) in the target organs to 
      participate in the disease process. Therefore, clarifying how gammadeltaT cells 
      chemotactically migrate to target organs may be a key to developing therapeutic 
      methods against LN. Enzyme-linked immunosorbent assay (ELISA) was used to detect 
      serum levels of chemokines in LN patients and healthy controls. Real-time 
      polymerase chain reaction (RT-PCR) and flow cytometry were used to measure the 
      expression of chemokine receptors on the surface of gammadeltaT cells. The chemotactic 
      migration ability of gammadeltaT cells was detected by Transwell assay. Signalling 
      pathway activation of gammadeltaT cells was detected by Automated Capillary 
      Electrophoresis Immunoassay and flow cytometry. The serum levels of chemokines, 
      including monocyte chemoattractant protein-1 (MCP-1) in LN patients, were 
      significantly increased. CCR2, the receptor of MCP-1, was also highly expressed 
      on the surface of peripheral gammadeltaT cells in LN patients. In addition, the exogenous 
      addition of MCP-1 can enhance chemotactic migration of gammadeltaT cells in LN patients. 
      MCP-1 could activate STAT3 signalling in LN patients' peripheral gammadeltaT cells. gammadeltaT 
      cells might participate in the pathogenesis of LN through MCP-1/CCR2 axis. This 
      finding provides new opportunities for developing treatment methods against LN by 
      targeting MCP-1/CCR2 axis.
CI  - (c) 2023 The Scandinavian Foundation for Immunology.
FAU - Deng, Ting
AU  - Deng T
AD  - Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, China.
FAU - Lei, Feifei
AU  - Lei F
AD  - Renmin Hospital, Hubei University of Medicine, Shiyan, China.
FAU - Wang, Zhongyu
AU  - Wang Z
AD  - Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, China.
FAU - Wang, Yangbin
AU  - Wang Y
AD  - Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, China.
FAU - Li, Gang
AU  - Li G
AD  - Renmin Hospital, Hubei University of Medicine, Shiyan, China.
FAU - Zhu, Yunhe
AU  - Zhu Y
AD  - Renmin Hospital, Hubei University of Medicine, Shiyan, China.
FAU - Du, Boyu
AU  - Du B
AD  - Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, China.
AD  - Renmin Hospital, Hubei University of Medicine, Shiyan, China.
FAU - Xi, Xueyan
AU  - Xi X
AUID- ORCID: 0000-0002-3164-9895
AD  - Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, China.
AD  - Renmin Hospital, Hubei University of Medicine, Shiyan, China.
AD  - Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of 
      Medicine, Shiyan, China.
LA  - eng
GR  - WJ2021M057/Health Commission of Hubei Province/
GR  - 202110929005/National Training Program of Innovation and Entrepreneurship for 
      Undergraduates/
GR  - 202110929020/National Training Program of Innovation and Entrepreneurship for 
      Undergraduates/
GR  - B2021166/Natural Science Foundation of Hubei Provincial Department of Education/
GR  - WDCM2020007/Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicien 
      Research (Hubei Unviersity of Medicine)/
GR  - 2021K62/The Scientific and Technological Project of Shiyan City of Hubei 
      Province/
PT  - Journal Article
DEP - 20230616
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Chemokine CCL2)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - Humans
MH  - Chemokine CCL2
MH  - *Lupus Nephritis
MH  - *Lupus Erythematosus, Systemic
MH  - Signal Transduction
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Receptors, CCR2
OTO - NOTNLM
OT  - CCR2
OT  - MCP-1
OT  - STAT3
OT  - lupus nephritis
OT  - gammadelta T cells
EDAT- 2024/03/05 12:48
MHDA- 2024/03/06 06:43
CRDT- 2024/03/05 10:09
PHST- 2023/05/03 00:00 [revised]
PHST- 2022/07/22 00:00 [received]
PHST- 2023/06/06 00:00 [accepted]
PHST- 2024/03/06 06:43 [medline]
PHST- 2024/03/05 12:48 [pubmed]
PHST- 2024/03/05 10:09 [entrez]
AID - 10.1111/sji.13305 [doi]
PST - ppublish
SO  - Scand J Immunol. 2023 Oct;98(4):e13305. doi: 10.1111/sji.13305. Epub 2023 Jun 16.