PMID- 38445171
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240307
IS  - 2215-0161 (Print)
IS  - 2215-0161 (Electronic)
IS  - 2215-0161 (Linking)
VI  - 12
DP  - 2024 Jun
TI  - In vitro determination of osteo-adipogenic lineage choice of bone marrow 
      stromal/stem cells (BMSCs).
PG  - 102637
LID - 10.1016/j.mex.2024.102637 [doi]
LID - 102637
AB  - Bone marrow stromal/stem cells (BMSCs) are primitive and heterogeneous cells that 
      can be differentiated into osteoblasts, adipocytes and other subsets. Their 
      bone-fat lineage commitment is responsible for the homeostasis of bone marrow 
      microenvironment. However, there are little effective methods and evidence to 
      simultaneously visualise the lineage commitment of BMSCs. Here we provide a 
      bivalent differentiation medium that can enable BMSCs differentiation into 
      osteoblasts and adipocytes in vitro, and establish a method to simultaneously 
      distinguish osteoblasts or adipocytes from the heterogeneous BMSCs based on 
      Alizarin red S and Oil red O staining, which have been used for detection of 
      specific mineralized nodules and lipid droplets, respectively. This assay 
      provides a specifically simple but effective and low-cost method to evaluate the 
      efficiency of osteo-adipogenic (OA) allocation of BMSCs.►Researchers can utilize 
      the bivalent differentiation medium to evaluate the efficiency of osteogenic and 
      adipogenic differentiation of BMSCs in vitro.
CI  - (c) 2024 The Authors. Published by Elsevier B.V.
FAU - Qiu, Dawei
AU  - Qiu D
AD  - Department of Physical Education, Guangxi University of Chinese Medicine, 
      Guangxi, Nanning 530200, China.
FAU - Wei, Wanyi
AU  - Wei W
AD  - Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, 
      Guangxi, Nanning 530200, China.
FAU - Chen, Jia
AU  - Chen J
AD  - Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, 
      Guangxi, Nanning 530200, China.
FAU - Huang, Jingwen
AU  - Huang J
AD  - Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, 
      Guangxi, Nanning 530200, China.
FAU - Yang, Yong
AU  - Yang Y
AD  - Faculty of Nursing, Guangxi University of Chinese Medicine, Guangxi, Nanning 
      530200, China.
FAU - Luo, Ziwei
AU  - Luo Z
AD  - College of Orthopedics, Guangxi University of Chinese Medicine, Guangxi, Nanning 
      530200, China.
LA  - eng
PT  - Journal Article
DEP - 20240227
PL  - Netherlands
TA  - MethodsX
JT  - MethodsX
JID - 101639829
PMC - PMC10912731
OTO - NOTNLM
OT  - Alizarin red S staining
OT  - BMSCs
OT  - Lineage choice
OT  - Oil red O staining
OT  - Osteo-adipogenic differentiation
OT  - Visualization of osteo-adipogenic lineage choice in cultured BMSCs
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2024/03/06 06:42
MHDA- 2024/03/06 06:43
PMCR- 2024/02/27
CRDT- 2024/03/06 04:01
PHST- 2024/01/13 00:00 [received]
PHST- 2024/02/26 00:00 [accepted]
PHST- 2024/03/06 06:43 [medline]
PHST- 2024/03/06 06:42 [pubmed]
PHST- 2024/03/06 04:01 [entrez]
PHST- 2024/02/27 00:00 [pmc-release]
AID - S2215-0161(24)00091-8 [pii]
AID - 102637 [pii]
AID - 10.1016/j.mex.2024.102637 [doi]
PST - epublish
SO  - MethodsX. 2024 Feb 27;12:102637. doi: 10.1016/j.mex.2024.102637. eCollection 2024 
      Jun.