PMID- 38448373
OWN - NLM
STAT- MEDLINE
DCOM- 20240308
LR  - 20240321
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 48
IP  - 12
DP  - 2023 Dec 28
TI  - Association between HLA-B*27:04 and genetic susceptibility to ankylosing 
      spondylitis in Hunan Province.
PG  - 1804-1811
LID - 1672-7347(2023)12-1804-08 [pii]
LID - 10.11817/j.issn.1672-7347.2023.230171 [doi]
AB  - OBJECTIVES: Human leukocyte antigen (HLA) B27 is a susceptibility allele of 
      ankylosing spondylitis (AS), and HLA-B27 antigen typing is an important indicator 
      for clinical diagnosis of AS, but current typing methods such as sequence 
      specific primer polymerase chain reaction (PCR-SSP) still possess limitation. 
      Therefore, this study aims to analyze the correlation between B27 subtypes and 
      susceptibility to AS in Hunan Province by applying high-resolution polymerase 
      chain reaction-sequence-based typing (PCR-SBT). METHODS: Peripheral blood of 116 
      patients with suspected AS (suspected AS group) and 121 healthy volunteers 
      (control group) admitted to the Second Xiangya Hospital from January 2020 to 
      December 2020 were collected for HLA-B genotyping by PCR-SBT. Among the patients 
      in the suspected AS group, 23 patients were finally diagnosed with AS (confirmed 
      AS group), and the remaining 93 undiagnosed patients served as the non-confirmed 
      AS group. PCR-SBT and PCR-SSP were used to detect HLA-B27 typing in 116 patients 
      with suspected AS, and the results of the 2 methods were compared. RESULTS: The 
      HLA-B27 allele frequency in the suspected AS group was significantly higher than 
      that in the control group [11.63% vs 2.48%; P<0.001, odds ratio (OR)=5.18, 95% 
      confidence interval (CI) 2.097 to 12.795]. B*27:04, B*27:05, B*27:06, and B*27:07 
      were detected in the suspected AS group and the control group. The frequency of 
      the B*27:04 allele in the suspected AS group was significantly higher than that 
      in the control group (9.48% vs 1.24%; P<0.001, OR=8.346, 95% CI 2.463 to 28.282). 
      The positive rate of B27 in the suspected AS group and the confirmed AS group 
      (B27+/+ and B27+/-) was significantly higher than that in the control group 
      (chi(2)=16.579, P<0.001; chi(2)=94.582, P<0.001, respectively). Among the confirmed 
      AS group, 21 were HLA-B27 carriers, and the B27 positive rate in the confirmed AS 
      group was 91.3%. PCR-SBT could achieve high resolution typing of the HLA-B gene 
      locus, with higher sensitivity, specificity, positive predictive value, negative 
      predictive value, and accuracy than PCR-SSP. CONCLUSIONS: PCR-SBT typing analysis 
      shows a strong correlation between HLA-B * 27:04 and AS in Hunan province. The 
      PCR-SBT method can be used as the preferred option for the auxiliary diagnosis of 
      clinical AS.
FAU - Lu, Xiaofang
AU  - Lu X
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013. 461283334@qq.com.
FAU - Wan, Ling
AU  - Wan L
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Zou, Kevin
AU  - Zou K
AD  - Xiangya School of Medicine, Central South University, Changsha 410013.
FAU - Tan, Liang
AU  - Tan L
AD  - Organ Transplant Center, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Zhu, Quan
AU  - Zhu Q
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Liu, Rongjiao
AU  - Liu R
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Yin, Xiangli
AU  - Yin X
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Song, Zixuan
AU  - Song Z
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Wei, Leiyan
AU  - Wei L
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Xiang, Zhiqing
AU  - Xiang Z
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013.
FAU - Zou, Yizhou
AU  - Zou Y
AD  - Department of Immunology, School of Basic Medical Sciences, Central South 
      University, Changsha 410013. yizhou_zou@sina.com.
LA  - eng
LA  - chi
GR  - 82171763/the National Natural Science Foundation of China/
PT  - Journal Article
TT  - 湖南地区携带HLA-B*27꞉04等位基因人群与强直性脊柱炎的易感性具有强相关性（英文）.
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (HLA-B27 Antigen)
SB  - IM
MH  - Humans
MH  - *HLA-B27 Antigen/genetics
MH  - *Spondylitis, Ankylosing/genetics
MH  - Genetic Predisposition to Disease
MH  - Genetic Testing
MH  - Gene Frequency
PMC - PMC10930755
OTO - NOTNLM
OT  - ankylosing spondylitis
OT  - genetic susceptibility
OT  - human leukocyte antigen-B*27:04
OT  - polymerase chain reaction sequence-based typing
COIS- The authors declare that they have no conflicts of interest to disclose.
EDAT- 2024/03/07 00:42
MHDA- 2024/03/08 06:42
PMCR- 2023/12/28
CRDT- 2024/03/06 22:33
PHST- 2024/03/08 06:42 [medline]
PHST- 2024/03/07 00:42 [pubmed]
PHST- 2024/03/06 22:33 [entrez]
PHST- 2023/12/28 00:00 [pmc-release]
AID - 1672-7347(2023)12-1804-08 [pii]
AID - 1672-7347（2023）12-1804-08 [pii]
AID - 10.11817/j.issn.1672-7347.2023.230171 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2023 Dec 28;48(12):1804-1811. doi: 
      10.11817/j.issn.1672-7347.2023.230171.