PMID- 38451182
OWN - NLM
STAT- MEDLINE
DCOM- 20240322
LR  - 20240329
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 16
IP  - 5
DP  - 2024 Mar 6
TI  - The low expression of miR-155 promotes the expression of SHP2 by inhibiting the 
      activation of the ERK1/2 pathway and improves cell pyroptosis induced by I/R in 
      mice.
PG  - 4778-4788
LID - 10.18632/aging.205631 [doi]
AB  - This study aims to explore the specific mechanism by which miR-155 regulates SHP2 
      expression in mouse ischemia-reperfusion (I/R) induced necroptosis. Various 
      methods including cardiac ultrasound, TTC staining, Masson staining, TUNEL 
      staining, and Western blotting were used to examine changes in the morphology and 
      function of the rat left ventricle, myocardial fibrosis, as well as the 
      expression of proteins related to tissue and cardiomyocyte necroptosis pathways. 
      In vivo results showed that knockdown (KD) of miR-155 significantly improved 
      cardiac ultrasound parameters (EF, FS, LVAW;d, and LVAW;s), reduced the 
      myocardial infarction area, myocardial fibrosis, and cell apoptosis in I/R mice, 
      upregulated cardiac SHP2 protein expression, and other proteins including 
      p-ERK1/2, NLRP3, GSDMD, caspase-3, caspase-4, and caspase-11 were also 
      significantly decreased. In vitro experiments showed that compared with the SHP2 
      WT miR-155 KD group, SHP2 protein expression was significantly increased in the 
      SHP2 WT miR-155 KD group, while the expression of other proteins was 
      significantly reduced, consistent with in vivo results. MiR-155 can regulate 
      ERK1/2 and NLRP3 through SHP2. After adding the ERK1/2 inhibitor U0126 to 
      cardiomyocytes from SHP2 KO mice, it was found that the expression of proteins 
      other than SHP2 significantly decreased compared to SHP2 KO cells without the 
      inhibitor. In summary, low expression of miR-155 promoted the expression of SHP2 
      and improved mouse I/R-induced necroptosis by inhibiting the activation of the 
      ERK1/2 pathway.
FAU - Liu, Mengru
AU  - Liu M
AD  - Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, 
      Beijing 100029, China.
FAU - Fu, Dongliang
AU  - Fu D
AD  - Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, 
      Beijing 100029, China.
FAU - Gao, Tong
AU  - Gao T
AD  - Department of Cardiology, Beijing Tsinghua Changgung Hospital, Beijing 100029, 
      China.
FAU - Jiang, Hong
AU  - Jiang H
AD  - Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, 
      Beijing 100029, China.
FAU - Yang, Peng
AU  - Yang P
AD  - Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, 
      Beijing 100029, China.
FAU - Li, Xianlun
AU  - Li X
AD  - Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, 
      Beijing 100029, China.
LA  - eng
PT  - Journal Article
DEP - 20240306
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (MIRN155 microRNA, rat)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - EC 3.1.3.48 (Ptpn11 protein, mouse)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Rats
MH  - Fibrosis
MH  - MAP Kinase Signaling System
MH  - *MicroRNAs/metabolism
MH  - *Myocardial Infarction/metabolism
MH  - Myocytes, Cardiac/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Pyroptosis
MH  - Reperfusion
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism
PMC - PMC10968689
OTO - NOTNLM
OT  - I/R
OT  - SHP2
OT  - miR-155
OT  - pyroptosis
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2024/03/07 12:43
MHDA- 2024/03/22 06:44
PMCR- 2024/03/15
CRDT- 2024/03/07 09:52
PHST- 2023/06/16 00:00 [received]
PHST- 2024/01/26 00:00 [accepted]
PHST- 2024/03/22 06:44 [medline]
PHST- 2024/03/07 12:43 [pubmed]
PHST- 2024/03/07 09:52 [entrez]
PHST- 2024/03/15 00:00 [pmc-release]
AID - 205631 [pii]
AID - 10.18632/aging.205631 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2024 Mar 6;16(5):4778-4788. doi: 10.18632/aging.205631. Epub 
      2024 Mar 6.