PMID- 38455567
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240309
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 10
IP  - 5
DP  - 2024 Mar 15
TI  - Exploring the functional role of tRF-39-8HM2OSRNLNKSEKH9 in hepatocellular 
      carcinoma.
PG  - e27153
LID - 10.1016/j.heliyon.2024.e27153 [doi]
LID - e27153
AB  - Hepatocellular carcinoma (HCC) is associated with high morbidity and mortality 
      globally. tRNA-derived small RNAs (tsRNAs) have emerged as potential targets for 
      cancer treatment. However, the specific impact of tsRNAs on HCC remains 
      undiscovered. In this study, we aimed to investigate the biological significance 
      of tsRNAs in HCC. First, we screened the differentially expressed tsRNAs in HCC 
      tissues and normal tissues adjacent to the tumor (NAT) using high-throughput 
      sequencing and the results showed that tRF-39-8HM2OSRNLNKSEKH9 was more highly 
      expressed in HCC tissues than NATs. Agarose gel electrophoresis (AGE), 
      nuclear-cytoplasmic separation assays and fluorescence in situ hybridization 
      (FISH) were employed to assess the characterization of tRF-39-8HM2OSRNLNKSEKH9. 
      The relationship between the expression of tRF-39-8HM2OSRNLNKSEKH9 and 
      clinicopathological parameters was evaluated and we found that it was positively 
      associated with tumor size. The cell counting kit-8 (CCK8) assay, colony 
      formation assay and EdU staining assay were employed to investigate the role of 
      tRF-39-8HM2OSRNLNKSEKH9 in the proliferation of HCC cells. Additionally, 
      transwell assays demonstrated that overexpression of tRF-39-8HM2OSRNLNKSEKH9 
      could accelerate cell migration capability. Taken together, 
      tRF-39-8HM2OSRNLNKSEKH9 was highly expressed in HCC cells, serum and tissues, and 
      it may play an oncogenic role in HCC cells through interacting with downstream 
      mRNA targets.
CI  - (c) 2024 The Authors.
FAU - Xu, Tianxin
AU  - Xu T
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      School of Nantong University, Nantong, China.
FAU - Yuan, Jie
AU  - Yuan J
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nantong University, 
      Medical School of Nantong University, Nantong, China.
FAU - Song, Fei
AU  - Song F
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      School of Nantong University, Nantong, China.
FAU - Zhang, Nannan
AU  - Zhang N
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      School of Nantong University, Nantong, China.
FAU - Gao, Cheng
AU  - Gao C
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      School of Nantong University, Nantong, China.
FAU - Chen, Zhong
AU  - Chen Z
AD  - Department of General Surgery, Affiliated Hospital of Nantong University, Medical 
      School of Nantong University, Nantong, China.
LA  - eng
PT  - Journal Article
DEP - 20240227
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10918225
OTO - NOTNLM
OT  - Hepatocellular carcinoma
OT  - Invasion
OT  - Migration
OT  - Oncogene
OT  - Proliferation
OT  - tRNA-derived small RNAs
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2024/03/08 06:42
MHDA- 2024/03/08 06:43
PMCR- 2024/02/27
CRDT- 2024/03/08 04:02
PHST- 2023/07/29 00:00 [received]
PHST- 2024/02/14 00:00 [revised]
PHST- 2024/02/26 00:00 [accepted]
PHST- 2024/03/08 06:43 [medline]
PHST- 2024/03/08 06:42 [pubmed]
PHST- 2024/03/08 04:02 [entrez]
PHST- 2024/02/27 00:00 [pmc-release]
AID - S2405-8440(24)03184-0 [pii]
AID - e27153 [pii]
AID - 10.1016/j.heliyon.2024.e27153 [doi]
PST - epublish
SO  - Heliyon. 2024 Feb 27;10(5):e27153. doi: 10.1016/j.heliyon.2024.e27153. 
      eCollection 2024 Mar 15.