PMID- 38462357
OWN - NLM
STAT- MEDLINE
DCOM- 20240312
LR  - 20240312
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 104
IP  - 11
DP  - 2024 Mar 19
TI  - [Challenges and solutions in current human leukocyte antigen confirmatory typing 
      of hematopoietic stem cell transplantation].
PG  - 793-798
LID - 10.3760/cma.j.cn112137-20231130-01243 [doi]
AB  - The impact of human leukocyte antigen (HLA) on hematopoietic stem cell 
      transplantation (HSCT) necessitates high precision in HLA genotyping. 
      Confirmatory typing for patients and their related or unrelated donors before 
      HSCT is critical. This study seeks to standardize HLA confirmatory typing in 
      laboratories by examining the current state of HLA genotyping in the country, 
      building upon the National Standards and Industrial Standards for HLA, and 
      highlighting the significance of confirmatory typing for patients and potential 
      donors prior to HSCT. A retrospective analysis over a decade reveals initial 
      typing errors, indicating potential issues and critical considerations in 
      pre-analytical, analytical, and post-analytical stages. Problems are attributed 
      to three main causes: (1) random human errors, including technical mistakes, 
      sample mix-up, and transcription inaccuracies; (2) limitations of technical 
      methods, such as the varied sequence ranges between confirmatory and initial 
      typing; (3) patient factors, involving high tumor burden, the influence of 
      certain drugs on HLA genotyping results, and the second transplantation. 
      Solutions are proposed for these problems, along with recommendations to 
      standardize HLA confirmatory typing.
FAU - Bao, X J
AU  - Bao XJ
AD  - HLA Laboratory, Jiangsu Institute of Hematology, the First Affiliated Hospital of 
      Soochow University, Suzhou 215031, China.
FAU - Yuan, X N
AU  - Yuan XN
AD  - HLA Laboratory, Jiangsu Institute of Hematology, the First Affiliated Hospital of 
      Soochow University, Suzhou 215031, China.
FAU - He, J
AU  - He J
AD  - HLA Laboratory, Jiangsu Institute of Hematology, the First Affiliated Hospital of 
      Soochow University, Suzhou 215031, China.
LA  - chi
GR  - SX21100121/Collaborative Innovation Center of Hematology of Soochow University/
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Humans
MH  - Retrospective Studies
MH  - *Hematopoietic Stem Cell Transplantation
MH  - HLA Antigens
MH  - Histocompatibility Testing/methods
MH  - Tissue Donors
MH  - Histocompatibility Antigens Class I
EDAT- 2024/03/11 00:42
MHDA- 2024/03/12 06:42
CRDT- 2024/03/10 22:18
PHST- 2024/03/12 06:42 [medline]
PHST- 2024/03/11 00:42 [pubmed]
PHST- 2024/03/10 22:18 [entrez]
AID - 10.3760/cma.j.cn112137-20231130-01243 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2024 Mar 19;104(11):793-798. doi: 
      10.3760/cma.j.cn112137-20231130-01243.