PMID- 38462361
OWN - NLM
STAT- MEDLINE
DCOM- 20240312
LR  - 20240312
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 104
IP  - 11
DP  - 2024 Mar 19
TI  - [Risk prediction and function evaluation by T-cell epitope model and expression 
      model of HLA-DPB1 mismatching in unrelated-donor hematopoietic stem cell 
      transplantations].
PG  - 850-856
LID - 10.3760/cma.j.cn112137-20231203-01275 [doi]
AB  - Objective: To evaluate the risk prediction and assessment function of HLA-DPB1 
      T-cell epitope (TCE) model and expression model in human leukocyte antigen 
      (HLA)-matched unrelated hematopoietic stem cell transplantation (MUD-HSCT) with 
      HLA-DPB1 mismatching. Methods: A total of 364 (182 pairs) potential MUD-HSCT 
      donors and recipients confirmed by HLA high-resolution typing in Shaanxi Blood 
      Center from 2016 to 2019 were analyzed retrospectively. Of the 182 recipients, 
      there were 121 males and 61 females with an average age of (26.3+/-14.2) years. Of 
      the 182 donors, there were 148 males and 34 females with an average age of 
      (33.7+/-7.5) years. Polymerase chain reaction-sequence-based typing (PCR-SBT), 
      next-generation sequencing (NGS) and polymerase chain reaction-sequence specific 
      oligonucleotide probe (PCR-SSO) based on LABScan((R))3D platform were used for 
      high-resolution typing of HLA-A, B, C, DRB1, DQB1, DPB1 gene, and PCR-SBT was 
      used for single nucleotide polymorphism (SNP) typing. TCE model and expression 
      model were used to predict and evaluate the HLA-DPB1 mismatch pattern and acute 
      graft-versus-host-disease (aGVHD) risk. Results: A total of 26 HLA-DPB1 alleles 
      and their 3'-UTR rs9277534 SNP genotypes were detected in this study population, 
      and two new alleles HLA-DPB1*1052ratio01 and HLA-DPB1*1119ratio01 were found and 
      officially named. The overall mismatch rate of HLA-DPB1 in MUD-HSCT donors and 
      recipients was 90.66% (165/182). In TCE model, the HLA-DPB1 mismatch rates of 
      permissible mismatch (PM) and non-permissible mismatch (non-PM) were 47.80% 
      (87/182) and 42.86% (78/182), respectively. The non-PM in GvH direction was 
      13.73% (25/182), and which in HvG direction was 29.12% (53/182). A total of 73 
      pairs of donors and recipients in TCE model met the evaluation criteria of 
      expression model. Among of TCE PM group, recipient DP5 mismatches accounted for 
      34.25% (25/73) were predicted as aGVHD high risk according to expression model. 
      For the TCE non-PM group, both the recipient DP2 mismatches of 6.85% (5/73) and 
      recipient DP5 mismatches of 10.86% (8/73) were predicted to be at high risk for 
      aGVHD. Risk prediction by TCE model and expression model was 27.27% concordant 
      and 16.97% unconcordant. Conclusions: TCE model and expression model are 
      effective tools to predict aGVHD risk of MUD-HSCT. Comprehensive application of 
      the two models is helpful to the hierarchical assessment of HSCT risk.
FAU - Qi, J
AU  - Qi J
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Wang, T J
AU  - Wang TJ
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Wang, M N
AU  - Wang MN
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Shang, L X
AU  - Shang LX
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Chen, L
AU  - Chen L
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Wang, X F
AU  - Wang XF
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Li, Y H
AU  - Li YH
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Xu, H
AU  - Xu H
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
FAU - Ma, C F
AU  - Ma CF
AD  - Blood Center of Shaanxi Province, Institute of Xi'an Blood Bank, Xi'an 710061, 
      China.
LA  - chi
GR  - CSBT-WG-2017-06/Weigao Research Fundation of Chinese Society of Blood 
      Transfusion/
GR  - 2022SF-098/General Project of Key Research and Development Program of Shaanxi 
      Province/
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (HLA-DPB1 antigen)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-DP beta-Chains)
SB  - IM
MH  - Male
MH  - Female
MH  - Humans
MH  - Child
MH  - Adolescent
MH  - Young Adult
MH  - Adult
MH  - Epitopes, T-Lymphocyte/genetics
MH  - Retrospective Studies
MH  - *Hematopoietic Stem Cell Transplantation
MH  - HLA-DP beta-Chains/genetics
MH  - Unrelated Donors
MH  - *Graft vs Host Disease/genetics
EDAT- 2024/03/11 00:42
MHDA- 2024/03/12 06:43
CRDT- 2024/03/10 22:18
PHST- 2024/03/12 06:43 [medline]
PHST- 2024/03/11 00:42 [pubmed]
PHST- 2024/03/10 22:18 [entrez]
AID - 10.3760/cma.j.cn112137-20231203-01275 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2024 Mar 19;104(11):850-856. doi: 
      10.3760/cma.j.cn112137-20231203-01275.