PMID- 38462492
OWN - NLM
STAT- MEDLINE
DCOM- 20240312
LR  - 20240312
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 47
IP  - 3
DP  - 2024
TI  - Causes of Sex Differences in Serum Cholesterol and Triglyceride Levels in Meishan 
      Pigs.
PG  - 606-610
LID - 10.1248/bpb.b23-00895 [doi]
AB  - To clarify the causes of sex differences (male < female) in the serum total 
      cholesterol (TCHO) and triglyceride (TG) levels in Meishan pigs, we examined the 
      sex differences in mRNA levels of key hepatic enzymes involved in the 
      biosynthesis/metabolism of cholesterol and TG using real-time RT-PCR. There were 
      no sex differences in mRNA levels of 3-hydroxy-3-methylglutaryl-CoA reductase and 
      CYP51A1 for cholesterol biosynthesis, or of the rate-limiting enzyme CYP7A1 for 
      bile acid synthesis from cholesterol. By contrast, sex differences 
      (male < female) were observed in mRNA levels of glycerol-3-phosphate 
      acyltransferase 1 (GPAT1), a rate-limiting enzyme for TG biosynthesis. However, 
      the sex differences in mRNA levels of carnitine palmitoyltransferase 1A (CPT1A) 
      and acyl-CoA dehydrogenase long chain (ACADL), key enzymes for the oxidation of 
      the fatty acids that are structural components of TG, were the opposite 
      (male > female). Castration of male pigs led to an increase in the mRNA level of 
      GPAT1 and decreases in those of CPT1A and ACADL. Furthermore, testosterone 
      propionate (TP)-treatment of castrated males and intact females restored and 
      changed, respectively, these mRNA levels to those of intact males. Notably, 
      castration and TP-treatment increased and decreased, respectively, serum and 
      hepatic TG levels. These findings suggest that sex differences in the serum and 
      hepatic TG levels in Meishan pigs are closely correlated with differences in 
      testosterone-associated mRNA expression levels of the key enzymes (GPAT1, CPT1A, 
      and ACADL) involved in the TG biosynthesis process, although no causes of sex 
      differences in serum and hepatic TCHO levels could be found.
FAU - Kojima, Misaki
AU  - Kojima M
AD  - Meat Animal Biosystem Group, Division of Meat Animal and Poultry Research, 
      Institute of Livestock and Grassland Science, National Agriculture and Food 
      Research Organization (NARO).
FAU - Degawa, Masakuni
AU  - Degawa M
AD  - Laboratory of Molecular Toxicology, School of Pharmaceutical Sciences, University 
      of Shizuoka.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 97C5T2UQ7J (Cholesterol)
RN  - WI93Z9138A (Testosterone Propionate)
RN  - 0 (Triglycerides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Swine
MH  - Female
MH  - Male
MH  - Animals
MH  - *Sex Characteristics
MH  - Liver/metabolism
MH  - Cholesterol
MH  - *Testosterone Propionate
MH  - Triglycerides
MH  - RNA, Messenger/metabolism
MH  - Lipid Metabolism
OTO - NOTNLM
OT  - androgen
OT  - hepatic gene expression
OT  - lipid biosynthesis enzyme
OT  - pig
OT  - serum lipid level
OT  - sex difference
EDAT- 2024/03/11 00:42
MHDA- 2024/03/12 06:43
CRDT- 2024/03/10 23:32
PHST- 2024/03/12 06:43 [medline]
PHST- 2024/03/11 00:42 [pubmed]
PHST- 2024/03/10 23:32 [entrez]
AID - 10.1248/bpb.b23-00895 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2024;47(3):606-610. doi: 10.1248/bpb.b23-00895.