PMID- 38463812 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240312 IS - 2405-8440 (Print) IS - 2405-8440 (Electronic) IS - 2405-8440 (Linking) VI - 10 IP - 5 DP - 2024 Mar 15 TI - Black phosphorus-based nanoparticles induce liver cancer cell mitochondrial apoptosis and immune cell tumor infiltration for enhancing dendritic cell therapy. PG - e27234 LID - 10.1016/j.heliyon.2024.e27234 [doi] LID - e27234 AB - Cellular immunotherapy is a crucial aspect of current tumor immunotherapy, though it presents several challenges such as immune cell dysfunction, limited recognition of neoantigens, and inadequate lymphocyte infiltration into the tumor microenvironment. This study proposes a novel approach utilizing a combination of dendritic cell (DC)-based cellular immunotherapy and a photothermal nanoadjuvant black phosphorus (BP) nanoparticles to overcome these challenges. A new platform called PLGA@BP-R848, which consists of modifying poly-(lactic-co-glycolic acid) (PLGA) onto BP nanosheets loading the immune adjuvant R848. The PLGA@BP-R848 nanoparticles demonstrated exceptional drug delivery and release capabilities, as well as a photothermal effect, biocompatibility, and the ability to activate the mitochondrial apoptotic pathway Blc-2-Bax-Cytochrome c-caspase-3 and inhibit the PI3K-AKT-mTOR signaling pathway. In a hepatocellular carcinoma mouse model, the binding of PLGA@BP-R848 nanoparticles and dendritic cells primed with GPC3 peptides, successfully induced a systemic anti-tumor immune response. PLGA@BP-R848 nanoparticles bolster immune cell infiltration into tumors and induce cancer cell apoptosis. The synergistic therapy involving dendritic cells and photothermal nanoadjuvant effectively suppressed tumor growth, and facilitated the formation of tertiary lymphatic structures (TLS) in tumors. This study presents a novel approach in using photothermal nanoadjuvants to advance antitumor effect of cellular immunotherapy, such as DCs therapy. CI - (c) 2024 The Authors. FAU - Liao, Ke AU - Liao K AD - Institute of Pharmacy and Pharmacology, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China. AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Chen, Shang AU - Chen S AD - Institute of Pharmacy and Pharmacology, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China. AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. AD - Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen, 518060, PR China. FAU - Yang, Gun AU - Yang G AD - Institute of Pharmacy and Pharmacology, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China. AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Huang, Xin AU - Huang X AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. AD - Department of Thoracic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, 230032, PR China. FAU - Wang, Tianyuan AU - Wang T AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Long, Shoubin AU - Long S AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Wang, Jing AU - Wang J AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Yin, Lei AU - Yin L AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. FAU - Zou, Qingshuang AU - Zou Q AD - Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China. FAU - Liu, Quan AU - Liu Q AD - Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen University, Shenzhen, 518052, PR China. AD - Division of Hepatobiliary and Pancreas Surgery, Department of General Surgery, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University), Shenzhen, 518020, PR China. FAU - Guo, Zifen AU - Guo Z AD - Institute of Pharmacy and Pharmacology, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China. LA - eng PT - Journal Article DEP - 20240302 PL - England TA - Heliyon JT - Heliyon JID - 101672560 PMC - PMC10923724 OTO - NOTNLM OT - Black phosphorus OT - Dendritic cells OT - Liver cancer OT - Photothermal therapy OT - Tertiary lymphatic structures COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/03/11 06:42 MHDA- 2024/03/11 06:43 PMCR- 2024/03/02 CRDT- 2024/03/11 04:27 PHST- 2024/01/25 00:00 [received] PHST- 2024/02/23 00:00 [revised] PHST- 2024/02/26 00:00 [accepted] PHST- 2024/03/11 06:43 [medline] PHST- 2024/03/11 06:42 [pubmed] PHST- 2024/03/11 04:27 [entrez] PHST- 2024/03/02 00:00 [pmc-release] AID - S2405-8440(24)03265-1 [pii] AID - e27234 [pii] AID - 10.1016/j.heliyon.2024.e27234 [doi] PST - epublish SO - Heliyon. 2024 Mar 2;10(5):e27234. doi: 10.1016/j.heliyon.2024.e27234. eCollection 2024 Mar 15.