PMID- 38464211
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240510
DP  - 2024 May 2
TI  - Prenatal Exposure to Environmentally Relevant Low Dosage Dibutyl Phthalate 
      Reduces Placental Efficiency in CD-1 Mice.
LID - 2024.02.26.582170 [pii]
LID - 10.1101/2024.02.26.582170 [doi]
AB  - INTRODUCTION: Dibutyl phthalate (DBP), a phthalate congener, is widely utilized 
      in consumer products and medication coatings. Women of reproductive age have a 
      significant burden of DBP exposure through consumer products, occupational 
      exposure, and medication. Prenatal DBP exposure is associated with adverse 
      pregnancy/fetal outcomes and cardiovascular diseases in the offspring. However, 
      the role of fetal sex and the general mechanisms underlying DBP 
      exposure-associated adverse pregnancy outcomes are unclear. We hypothesize that 
      prenatal DBP exposure at an environmentally relevant low dosage adversely affects 
      fetal-placental development and function during pregnancy in a fetal sex-specific 
      manner. METHODS: Adult female CD-1 mice (8-10wks) were orally treated with 
      vehicle (control) or with environmentally relevant low DBP dosages at 0.1 
      mug/kg/day (refer as DBP0.1) daily from 30 days before pregnancy through 
      gestational day (GD) 18.5. Dam body mass composition was measured non-invasively 
      using the echo-magnetic resonance imaging system. Lipid disposition in fetal 
      labyrinth and maternal decidual area of placentas was examined using Oil Red O 
      staining. RESULTS: DBP0.1 exposure did not significantly affect the body weight 
      and adiposity of non-pregnant adult female mice nor the maternal weight gain 
      pattern and adiposity during pregnancy in adult female mice. DBP0.1 exposure does 
      not affect fetal weight but significantly increased the placental weight at 
      GD18.5 (indicative of decreased placental efficiency) in a fetal sex-specific 
      manner. We further observed that DBP0.1 significantly decreased lipid disposition 
      in fetal labyrinth of female, but not male placentas, while it did not affect 
      lipid disposition in maternal decidual. CONCLUSIONS: Prenatal exposure to 
      environmentally relevant low-dosage DBP adversely impacts the fetal-placental 
      efficiency and lipid disposition in a fetal sex-specific manner.
FAU - Pontifex, Tasha
AU  - Pontifex T
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
FAU - Yang, Xinran
AU  - Yang X
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
FAU - Tracy, Ayna
AU  - Tracy A
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
FAU - Burns, Kimberlie
AU  - Burns K
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
FAU - Craig, Zelieann
AU  - Craig Z
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
FAU - Zhou, Chi
AU  - Zhou C
AUID- ORCID: 0000-0002-3349-9069
AD  - School of Animal and Comparative Biomedical Sciences, The University of Arizona, 
      Tucson, AZ, United States.
AD  - Department of Obstetrics and Gynecology, The University of Arizona, Tucson, AZ, 
      United States.
LA  - eng
GR  - P30 ES006694/ES/NIEHS NIH HHS/United States
PT  - Preprint
DEP - 20240502
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10925143
OTO - NOTNLM
OT  - Dibutyl phthalate
OT  - Fetal Sex
OT  - Lipid Disposition
OT  - Placenta
OT  - Prenatal Exposure
COIS- Conflict of Interest/Disclosure Statement The authors have no conflict of 
      interest.
EDAT- 2024/03/11 06:42
MHDA- 2024/03/11 06:43
PMCR- 2024/05/03
CRDT- 2024/03/11 04:32
PHST- 2024/03/11 06:42 [pubmed]
PHST- 2024/03/11 06:43 [medline]
PHST- 2024/03/11 04:32 [entrez]
PHST- 2024/05/03 00:00 [pmc-release]
AID - 2024.02.26.582170 [pii]
AID - 10.1101/2024.02.26.582170 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2024 May 2:2024.02.26.582170. doi: 10.1101/2024.02.26.582170.