PMID- 38464608
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240312
IS  - 2008-8140 (Print)
IS  - 2322-3618 (Electronic)
IS  - 2008-8140 (Linking)
VI  - 15
IP  - 1
DP  - 2024
TI  - Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after 
      spinal cord injury.
PG  - 27-34
LID - 10.30466/vrf.2023.2003942.3903 [doi]
AB  - Spinal cord injury (SCI) presents challenging and unpredictable neurological 
      recovery. During inflammatory conditions, the amount of serum albumin and 
      nutrition consumption decreases. Currently, it is proposed to measure serum 
      albumin and glucose content in human or animal subjects to predict the recovery 
      rate and the efficiency of treatments following SCI. In this study, the effect of 
      extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived 
      mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the 
      human indoleamine 2,3-dioxygenase-1 (IDO1) gene on serum albumin and glucose 
      levels was investigated. After pre-clearing steps of 72-hr conditioned media, 
      small EVs (sEVs) were isolated based on the ultra-filtration method. They were 
      encapsulated with a chitosan-based hydrogel. Five experimental groups (female 
      rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control 
      green fluorescent protein (GFP)-EVs and IDO1-EVs. The 60.00 microL of hydrogel or 
      hydrogels containing 100 microg sEVs from GFP or IDO1-EVs were locally injected 
      immediately after SCI (laminectomy of the T10 vertebra and clip compression). 
      After 8 weeks, non-fasting serum glucose and albumin levels were measured. The 
      results indicated that the level of serum albumin in the animals received 
      IDO1-EVs (3.52 +/- 0.04) was increased in comparison with the SCI group (3.00 +/- 
      0.94). Also, these animals indicated higher glucose levels in their serum (250.17 
      +/- 69.61) in comparison with SCI ones (214 +/- 45.34). Although these changes were 
      not statistically significant, they could be considered as evidence for the 
      beneficial effects of IDO1-EVs administration in the context of SCI to reduce 
      hypoalbuminemia and improve energy consumption. More detailed experiments are 
      required to confirm these results.
CI  - (c) 2024 Urmia University. All rights reserved.
FAU - Amanollahi, Shiva
AU  - Amanollahi S
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi 
      University of Mashhad, Mashhad, Iran.
FAU - Bahrami, Ahmad Reza
AU  - Bahrami AR
AD  - Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
AD  - Industrial Biotechnology Research Group, Institute of Biotechnology, Ferdowsi 
      University of Mashhad, Mashhad, Iran.
FAU - Haghighitalab, Azadeh
AU  - Haghighitalab A
AD  - Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
AD  - Stem Cells and Regenerative Medicine Research Group, Academic Center for 
      Education, Culture and Research (ACECR), Mashhad, Iran.
FAU - Shaterzadeh Yazdi, Hanieh
AU  - Shaterzadeh Yazdi H
AD  - Private Veterinary Practitioner, Mashhad, Iran.
FAU - Kazemi Mehrjerdi, Hossein
AU  - Kazemi Mehrjerdi H
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi 
      University of Mashhad, Mashhad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20240115
PL  - Iran
TA  - Vet Res Forum
JT  - Veterinary research forum : an international quarterly journal
JID - 101625812
PMC - PMC10921135
OTO - NOTNLM
OT  - 3-dioxygenase-1
OT  - Extra-cellular vesicles
OT  - Glucose
OT  - Hypoalbunemia
OT  - Indoleamine 2
OT  - Spinal cord injury
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/11 06:43
MHDA- 2024/03/11 06:44
PMCR- 2024/01/01
CRDT- 2024/03/11 04:38
PHST- 2023/06/09 00:00 [received]
PHST- 2023/08/23 00:00 [accepted]
PHST- 2024/03/11 06:44 [medline]
PHST- 2024/03/11 06:43 [pubmed]
PHST- 2024/03/11 04:38 [entrez]
PHST- 2024/01/01 00:00 [pmc-release]
AID - 10.30466/vrf.2023.2003942.3903 [doi]
PST - ppublish
SO  - Vet Res Forum. 2024;15(1):27-34. doi: 10.30466/vrf.2023.2003942.3903. Epub 2024 
      Jan 15.