PMID- 38472149
OWN - NLM
STAT- MEDLINE
DCOM- 20240408
LR  - 20240408
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 23
IP  - 4
DP  - 2024 Apr 5
TI  - Proteomic Profiling of Messenger Ribonucleoproteins in Mouse Tissues Based on 
      Formaldehyde Cross-Linking.
PG  - 1370-1378
LID - 10.1021/acs.jproteome.3c00856 [doi]
AB  - Messenger ribonucleoprotein particles (mRNPs) are vital for tissue-specific gene 
      expression via mediating posttranscriptional regulations. However, proteomic 
      profiling of proteins in mRNPs, i.e., mRNA-associated proteins (mRAPs), has been 
      challenging at the tissue level. Herein, we report the development of 
      formaldehyde cross-linking-based mRNA-associated protein profiling (FAXRAP), a 
      chemical strategy that enables the identification of mRAPs in both cultured cells 
      and intact mouse organs. Applying FAXRAP, tissue-specific mRAPs were 
      systematically profiled in the mouse liver, kidney, heart, and brain. 
      Furthermore, brain mRAPs in Parkinson's disease (PD) mouse model were 
      investigated, which revealed a global decrease of mRNP assembly in the brain of 
      mice with PD. We envision that FAXRAP will facilitate uncovering the 
      posttranscriptional regulation networks in various biological systems.
FAU - Wang, Jiankun
AU  - Wang J
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
FAU - Liu, Jialin
AU  - Liu J
AUID- ORCID: 0000-0002-9450-0772
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
FAU - Huang, Rongbing
AU  - Huang R
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
FAU - Chu, Tianyu
AU  - Chu T
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
FAU - Tang, Qi
AU  - Tang Q
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
FAU - Chen, Xing
AU  - Chen X
AUID- ORCID: 0000-0002-3058-7370
AD  - College of Chemistry and Molecular Engineering, Beijing National Laboratory for 
      Molecular Sciences, Peking-Tsinghua Center for Life Sciences, Synthetic and 
      Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and 
      Molecular Engineering of Ministry of Education, Peking University, Beijing 
      100871, China.
LA  - eng
PT  - Journal Article
DEP - 20240312
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (messenger ribonucleoprotein)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (RNA, Messenger)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Proteomics
MH  - *Ribonucleoproteins/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Formaldehyde
OTO - NOTNLM
OT  - RNA-associated protein
OT  - formaldehyde cross-linking
OT  - messenger ribonucleoprotein particle
OT  - posttranscriptional regulation network
EDAT- 2024/03/13 06:47
MHDA- 2024/04/08 06:43
CRDT- 2024/03/13 00:12
PHST- 2024/04/08 06:43 [medline]
PHST- 2024/03/13 06:47 [pubmed]
PHST- 2024/03/13 00:12 [entrez]
AID - 10.1021/acs.jproteome.3c00856 [doi]
PST - ppublish
SO  - J Proteome Res. 2024 Apr 5;23(4):1370-1378. doi: 10.1021/acs.jproteome.3c00856. 
      Epub 2024 Mar 12.