PMID- 38472220
OWN - NLM
STAT- MEDLINE
DCOM- 20240314
LR  - 20240316
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 14
IP  - 1
DP  - 2024 Mar 12
TI  - Towards standardising retinal OCT angiography image analysis with open-source 
      toolbox OCTAVA.
PG  - 5979
LID - 10.1038/s41598-024-53501-6 [doi]
LID - 5979
AB  - Quantitative assessment of retinal microvasculature in optical coherence 
      tomography angiography (OCTA) images is important for studying, diagnosing, 
      monitoring, and guiding the treatment of ocular and systemic diseases. However, 
      the OCTA user community lacks universal and transparent image analysis tools that 
      can be applied to images from a range of OCTA instruments and provide reliable 
      and consistent microvascular metrics from diverse datasets. We present a retinal 
      extension to the OCTA Vascular Analyser (OCTAVA) that addresses the challenges of 
      providing robust, easy-to-use, and transparent analysis of retinal OCTA images. 
      OCTAVA is a user-friendly, open-source toolbox that can analyse retinal OCTA 
      images from various instruments. The toolbox delivers seven microvascular metrics 
      for the whole image or subregions and six metrics characterising the foveal 
      avascular zone. We validate OCTAVA using images collected by four commercial OCTA 
      instruments demonstrating robust performance across datasets from different 
      instruments acquired at different sites from different study cohorts. We show 
      that OCTAVA delivers values for retinal microvascular metrics comparable to the 
      literature and reduces their variation between studies compared to their 
      commercial equivalents. By making OCTAVA publicly available, we aim to expand 
      standardised research and thereby improve the reproducibility of quantitative 
      analysis of retinal microvascular imaging. Such improvements will help to better 
      identify more reliable and sensitive biomarkers of ocular and systemic diseases.
CI  - (c) 2024. The Author(s).
FAU - Untracht, Gavrielle R
AU  - Untracht GR
AD  - Department of Health Technology, Technical University of Denmark, 2800, Kongens 
      Lyngby, Denmark.
AD  - School of Biosciences, The University of Surrey, Guildford, GU27XH, UK.
FAU - Durkee, Madeleine S
AU  - Durkee MS
AD  - Department of Radiology, University of Chicago, Chicago, IL, 60637, USA.
FAU - Zhao, Mei
AU  - Zhao M
AD  - Centre for Myopia Research, School of Optometry, Faculty of Health and Social 
      Science, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, 
      China.
FAU - Kwok-Cheung Lam, Andrew
AU  - Kwok-Cheung Lam A
AD  - Centre for Myopia Research, School of Optometry, Faculty of Health and Social 
      Science, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, 
      China.
FAU - Sikorski, Bartosz L
AU  - Sikorski BL
AD  - Department of Ophthalmology, Nicolaus Copernicus University, 85-090, Bydgoszcz, 
      Poland.
AD  - International Center for Translational Eye Research (ICTER), Institute of 
      Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, 
      Poland.
FAU - Sarunic, Marinko V
AU  - Sarunic MV
AD  - Department of Medical Physics and Biomedical Engineering, University College 
      London, London, WC1E6BT, UK.
AD  - Institute of Ophthalmology, University College London, London, EC1V2PD, UK.
FAU - Andersen, Peter E
AU  - Andersen PE
AD  - Department of Health Technology, Technical University of Denmark, 2800, Kongens 
      Lyngby, Denmark.
FAU - Sampson, David D
AU  - Sampson DD
AD  - School of Computer Science and Electronic Engineering, The University of Surrey, 
      Guildford, GU27XH, UK.
FAU - Chen, Fred K
AU  - Chen FK
AD  - Centre for Ophthalmology and Visual Science (Incorporating Lions Eye Institute), 
      The University of Western Australia, Perth, WA, 6009, Australia.
AD  - Department of Ophthalmology, Royal Perth Hospital, Perth, WA, 6000, Australia.
AD  - Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, VIC, 
      3002, Australia.
FAU - Sampson, Danuta M
AU  - Sampson DM
AD  - School of Biosciences, The University of Surrey, Guildford, GU27XH, UK. 
      Danuta.sampson@uwa.edu.au.
AD  - Institute of Ophthalmology, University College London, London, EC1V2PD, UK. 
      Danuta.sampson@uwa.edu.au.
AD  - Centre for Ophthalmology and Visual Science (Incorporating Lions Eye Institute), 
      The University of Western Australia, Perth, WA, 6009, Australia. 
      Danuta.sampson@uwa.edu.au.
AD  - Department of Optometry, School of Allied Health, The University of Western 
      Australia, Perth, WA, 6009, Australia. Danuta.sampson@uwa.edu.au.
LA  - eng
GR  - 871212/Horizon 2020 Framework Programme/
PT  - Journal Article
DEP - 20240312
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - *Retinal Vessels
MH  - Reproducibility of Results
MH  - Fluorescein Angiography/methods
MH  - *Macula Lutea
MH  - Microvessels
MH  - Tomography, Optical Coherence/methods
PMC - PMC10933365
COIS- The authors declare no competing interests.
EDAT- 2024/03/13 06:47
MHDA- 2024/03/14 06:46
PMCR- 2024/03/12
CRDT- 2024/03/13 00:47
PHST- 2023/08/15 00:00 [received]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/03/14 06:46 [medline]
PHST- 2024/03/13 06:47 [pubmed]
PHST- 2024/03/13 00:47 [entrez]
PHST- 2024/03/12 00:00 [pmc-release]
AID - 10.1038/s41598-024-53501-6 [pii]
AID - 53501 [pii]
AID - 10.1038/s41598-024-53501-6 [doi]
PST - epublish
SO  - Sci Rep. 2024 Mar 12;14(1):5979. doi: 10.1038/s41598-024-53501-6.