PMID- 38472753
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240315
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 13
IP  - 5
DP  - 2024 Feb 20
TI  - Determination of Aflatoxin B(1) in Grains by Aptamer Affinity Column Enrichment 
      and Purification Coupled with High Performance Liquid Chromatography Detection.
LID - 10.3390/foods13050640 [doi]
LID - 640
AB  - Aflatoxin B(1) (AFB(1)) is a highly teratogenic and carcinogenic secondary 
      metabolite produced by Aspergillus. It is commonly detected in agricultural 
      products such as cereals, peanuts, corn, and feed. Grains have a complex 
      composition. These complex components severely interfere with the effective 
      extraction and separation of AFB(1), and also cause problems such as matrix 
      interference and instrument damage, thus posing a great challenge in the accurate 
      analysis of AFB(1). In this study, an aptamer affinity column for AFB(1) analysis 
      (AFB(1)-AAC) was prepared for the enrichment and purification of AFB(1) from 
      grain samples. AFB(1)-AAC with an AFB(1)-specific aptamer as the recognition 
      element exhibited high affinity and specificity for AFB(1). Grain samples were 
      enriched and purified by AFB(1)-AAC, and subsequently analyzed by high 
      performance liquid chromatography with post-column photochemical 
      derivatization-fluorescence detection (HPLC-PCD-FLD). The average recoveries of 
      AFB(1) ranged from 88.7% to 99.1%, with relative standard deviations (RSDs) of 
      1.4-5.6% (n = 3) at the spiked levels of 5.0-20.0 mug kg(-1). The limit of 
      detection (LOD) for AFB(1) (0.02 mug kg(-1)) was much below the maximum residue 
      limits (MRLs) for AFB(1). This novel method can be applied to the determination 
      of AFB(1) residues in peanut, corn, and rice.
FAU - Ji, Cong
AU  - Ji C
AD  - College of Food Science and Engineering, Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, China.
FAU - Sun, Xinyang
AU  - Sun X
AD  - College of Food Science and Engineering, Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, China.
FAU - Fang, Yong
AU  - Fang Y
AD  - College of Food Science and Engineering, Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, China.
FAU - Li, Peng
AU  - Li P
AUID- ORCID: 0000-0002-1574-1341
AD  - College of Food Science and Engineering, Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, China.
LA  - eng
GR  - 32072317/the National Natural Science Foundation of China/
GR  - 2021YFD2100601/the National Key Research and Development Program of China/
GR  - BE2021370/the Jiangsu Provincial Key Research and Development Project (Modern 
      Agriculture)/
GR  - BK20210672/the Natural Science Foundation of Jiangsu Province/
GR  - QKH-ZK [2021] 073/the Guizhou Basic Research Program (Science and Technology 
      Foundation)/
GR  - KF-2023001/the Key Laboratory of Tropical Fruits and Vegetables Quality and 
      Safety for State Market Regu-lation/
PT  - Journal Article
DEP - 20240220
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC10930453
OTO - NOTNLM
OT  - HPLC
OT  - aflatoxin B1
OT  - aptamer
OT  - grain
OT  - purification
COIS- All authors declare that they have no conflict of interest.
EDAT- 2024/03/13 06:46
MHDA- 2024/03/13 06:47
PMCR- 2024/02/20
CRDT- 2024/03/13 01:16
PHST- 2024/01/11 00:00 [received]
PHST- 2024/02/07 00:00 [revised]
PHST- 2024/02/16 00:00 [accepted]
PHST- 2024/03/13 06:47 [medline]
PHST- 2024/03/13 06:46 [pubmed]
PHST- 2024/03/13 01:16 [entrez]
PHST- 2024/02/20 00:00 [pmc-release]
AID - foods13050640 [pii]
AID - foods-13-00640 [pii]
AID - 10.3390/foods13050640 [doi]
PST - epublish
SO  - Foods. 2024 Feb 20;13(5):640. doi: 10.3390/foods13050640.