PMID- 38474201
OWN - NLM
STAT- MEDLINE
DCOM- 20240314
LR  - 20240315
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 25
IP  - 5
DP  - 2024 Mar 3
TI  - Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat 
      Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.
LID - 10.3390/ijms25052954 [doi]
LID - 2954
AB  - In recent years, the potent influence of tocotrienol (T3) on diminishing blood 
      glucose and lipid concentrations in both Mus musculus (rats) and Homo sapiens 
      (humans) has been established. However, the comprehensive exploration of 
      tocotrienol's hypolipidemic impact and the corresponding mechanisms in aquatic 
      species remains inadequate. In this study, we established a zebrafish model of a 
      type 2 diabetes mellitus (T2DM) model through high-fat diet administration to 
      zebrafish. In the T2DM zebrafish, the thickness of ocular vascular walls 
      significantly increased compared to the control group, which was mitigated after 
      treatment with T3. Additionally, our findings demonstrate the regulatory effect 
      of T3 on lipid metabolism, leading to the reduced synthesis and storage of 
      adipose tissue in zebrafish. We validated the expression patterns of genes 
      relevant to these processes using RT-qPCR. In the T2DM model, there was an almost 
      two-fold upregulation in ppargamma and cyp7a1 mRNA levels, coupled with a significant 
      downregulation in cpt1a mRNA (p < 0.01) compared to the control group. The ELISA 
      revealed that the protein expression levels of Ppargamma and Rxralpha exhibited a 
      two-fold elevation in the T2DM group relative to the control. In the T3-treated 
      group, Ppargamma and Rxralpha protein expression levels consistently exhibited a two-fold 
      decrease compared to the model group. Lipid metabolomics showed that T3 could 
      affect the metabolic pathways of zebrafish lipid regulation, including lipid 
      synthesis and decomposition. We provided experimental evidence that T3 could 
      mitigate lipid accumulation in our zebrafish T2DM model. Elucidating the 
      lipid-lowering effects of T3 could help to minimize the detrimental impacts of 
      overfeeding in aquaculture.
FAU - Liu, Naicheng
AU  - Liu N
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Xue, Mingyang
AU  - Xue M
AUID- ORCID: 0000-0003-3370-5712
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Zhang, Mengwei
AU  - Zhang M
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Huang, Zhenyu
AU  - Huang Z
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Xu, Chen
AU  - Xu C
AUID- ORCID: 0000-0002-5075-7154
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Meng, Yan
AU  - Meng Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Fan, Yuding
AU  - Fan Y
AUID- ORCID: 0000-0002-7291-2493
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Zhang, Feixiang
AU  - Zhang F
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
AD  - College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China.
FAU - Chen, Peng
AU  - Chen P
AD  - Institute of Fishery Research of Xinjiang Uygur Autonenous Region, Urumqi 830099, 
      China.
FAU - Zhou, Yong
AU  - Zhou Y
AUID- ORCID: 0000-0003-0373-5734
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
LA  - eng
GR  - 2022BBA0054, 2023BBB122/CARS-45, technical innovation special project of Hubei 
      Province/
GR  - 32202980/the national natural science foundation of China/
GR  - 2023TD46, YFI 202207/central public-interest scientific institution basal 
      research fund/
PT  - Journal Article
DEP - 20240303
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Tocotrienols)
RN  - LZO6K1506A (Rice Bran Oil)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Rats
MH  - Animals
MH  - *Tocotrienols/metabolism
MH  - Zebrafish/metabolism
MH  - Diet, High-Fat
MH  - *Hyperlipidemias/metabolism
MH  - Rice Bran Oil
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - PPAR gamma/metabolism
MH  - RNA, Messenger/metabolism
MH  - Lipid Metabolism
MH  - Liver/metabolism
PMC - PMC10931685
OTO - NOTNLM
OT  - blood fat
OT  - high-fat diet
OT  - rice bran oil extract
OT  - tocotrienol (T3)
OT  - type 2 diabetes mellitus (T2DM) model
OT  - zebrafish
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/13 06:46
MHDA- 2024/03/14 06:47
PMCR- 2024/03/03
CRDT- 2024/03/13 01:24
PHST- 2024/01/18 00:00 [received]
PHST- 2024/02/23 00:00 [revised]
PHST- 2024/02/24 00:00 [accepted]
PHST- 2024/03/14 06:47 [medline]
PHST- 2024/03/13 06:46 [pubmed]
PHST- 2024/03/13 01:24 [entrez]
PHST- 2024/03/03 00:00 [pmc-release]
AID - ijms25052954 [pii]
AID - ijms-25-02954 [pii]
AID - 10.3390/ijms25052954 [doi]
PST - epublish
SO  - Int J Mol Sci. 2024 Mar 3;25(5):2954. doi: 10.3390/ijms25052954.