PMID- 38474410
OWN - NLM
STAT- MEDLINE
DCOM- 20240314
LR  - 20240315
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 13
IP  - 5
DP  - 2024 Mar 3
TI  - Exploiting Leishmania-Primed Dendritic Cells as Potential Immunomodulators of 
      Canine Immune Response.
LID - 10.3390/cells13050445 [doi]
LID - 445
AB  - Dendritic cells (DCs) capture pathogens and process antigens, playing a crucial 
      role in activating naive T cells, bridging the gap between innate and acquired 
      immunity. However, little is known about DC activation when facing Leishmania 
      parasites. Thus, this study investigates in vitro activity of canine peripheral 
      blood-derived DCs (moDCs) exposed to L. infantum and L. amazonensis parasites and 
      their extracellular vesicles (EVs). L. infantum increased toll-like receptor 4 
      gene expression in synergy with nuclear factor kappaB activation and the generation 
      of pro-inflammatory cytokines. This parasite also induced the expression of class 
      II molecules of major histocompatibility complex (MHC) and upregulated 
      co-stimulatory molecule CD86, which, together with the release of chemokine 
      CXCL16, can attract and help in T lymphocyte activation. In contrast, L. 
      amazonensis induced moDCs to generate a mix of pro- and anti-inflammatory 
      cytokines, indicating that this parasite can establish a different immune 
      relationship with DCs. EVs promoted moDCs to express class I MHC associated with 
      the upregulation of co-stimulatory molecules and the release of CXCL16, 
      suggesting that EVs can modulate moDCs to attract cytotoxic CD8(+) T cells. Thus, 
      these parasites and their EVs can shape DC activation. A detailed understanding 
      of DC activation may open new avenues for the development of advanced 
      leishmaniasis control strategies.
FAU - Valerio-Bolas, Ana
AU  - Valerio-Bolas A
AUID- ORCID: 0000-0002-0273-4353
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Meunier, Mafalda
AU  - Meunier M
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Palma-Marques, Joana
AU  - Palma-Marques J
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Rodrigues, Armanda
AU  - Rodrigues A
AUID- ORCID: 0000-0003-4261-3879
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Santos, Ana Margarida
AU  - Santos AM
AD  - Divisao de Medicina Veterinaria, Guarda Nacional Republicana, 1200-771 Lisbon, 
      Portugal.
FAU - Nunes, Telmo
AU  - Nunes T
AD  - Microscopy Center, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, 
      Portugal.
FAU - Ferreira, Rui
AU  - Ferreira R
AD  - Banco de Sangue Animal (BSA), 4100-462 Porto, Portugal.
FAU - Armada, Ana
AU  - Armada A
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Alves, Joao Carlos
AU  - Alves JC
AUID- ORCID: 0000-0002-1329-3709
AD  - Divisao de Medicina Veterinaria, Guarda Nacional Republicana, 1200-771 Lisbon, 
      Portugal.
FAU - Antunes, Wilson
AU  - Antunes W
AD  - Unidade Militar Laboratorial de Defesa Biologica e Quimica (UMLDBQ), 1849-012 
      Lisbon, Portugal.
FAU - Cardoso, Ines
AU  - Cardoso I
AD  - Banco de Sangue Animal (BSA), 4100-462 Porto, Portugal.
FAU - Mesquita-Gabriel, Sofia
AU  - Mesquita-Gabriel S
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Lobo, Lis
AU  - Lobo L
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
FAU - Alexandre-Pires, Graca
AU  - Alexandre-Pires G
AUID- ORCID: 0000-0002-9462-4679
AD  - CIISA, Centre for Interdisciplinary Research in Animal Health, Faculty of 
      Veterinary Medicine, University of Lisbon, 1649-004 Lisbon, Portugal.
AD  - Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1200-771 
      Lisbon, Portugal.
FAU - Marques, Luis
AU  - Marques L
AD  - BioSystems and Integrative Sciences Institute, Faculty of Sciences, University of 
      Lisbon-FCUL-BioISI Ce3CE, 1749-016 Lisbon, Portugal.
FAU - Pereira da Fonseca, Isabel
AU  - Pereira da Fonseca I
AUID- ORCID: 0000-0002-6632-1224
AD  - CIISA, Centre for Interdisciplinary Research in Animal Health, Faculty of 
      Veterinary Medicine, University of Lisbon, 1649-004 Lisbon, Portugal.
AD  - Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1200-771 
      Lisbon, Portugal.
FAU - Santos-Gomes, Gabriela
AU  - Santos-Gomes G
AUID- ORCID: 0000-0001-9264-3887
AD  - Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation 
      and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina 
      Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
LA  - eng
GR  - PTDC/CVT-CVT/28908/2017 
      (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017)/Fundacao para a Ciencia e 
      Tecnologia/
GR  - PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020)/Fundacao 
      para a Ciencia e Tecnologia/
GR  - Centro de Investigacao Interdisciplinar em Sanidade Animal (CIISA, 
      UIDB/00276/2020)/Fundacao para a Ciencia e Tecnologia/
GR  - Global Health and Tropical Medicine (GHTM, UID/04413/2020)/Fundacao para a 
      Ciencia e Tecnologia/
GR  - LA-REAL - LA/P/0117/2020/Fundacao para a Ciencia e Tecnologia/
PT  - Journal Article
DEP - 20240303
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - *Leishmania
MH  - CD8-Positive T-Lymphocytes
MH  - Dendritic Cells
MH  - Adjuvants, Immunologic/metabolism
MH  - Cytokines/metabolism
MH  - Lymphocyte Activation
PMC - PMC10931270
OTO - NOTNLM
OT  - L. infantum
OT  - Leishmania amazonensis
OT  - Leishmania extracellular vesicles
OT  - cytokines
OT  - dog
OT  - innate immunity
OT  - major histocompatibility complex
OT  - pattern recognition receptors
OT  - peripheral blood-derived dendritic cells (moDCs)
COIS- The authors declare no conflicts of interest.
EDAT- 2024/03/13 06:46
MHDA- 2024/03/14 06:46
PMCR- 2024/03/03
CRDT- 2024/03/13 01:25
PHST- 2024/01/05 00:00 [received]
PHST- 2024/02/23 00:00 [revised]
PHST- 2024/02/26 00:00 [accepted]
PHST- 2024/03/14 06:46 [medline]
PHST- 2024/03/13 06:46 [pubmed]
PHST- 2024/03/13 01:25 [entrez]
PHST- 2024/03/03 00:00 [pmc-release]
AID - cells13050445 [pii]
AID - cells-13-00445 [pii]
AID - 10.3390/cells13050445 [doi]
PST - epublish
SO  - Cells. 2024 Mar 3;13(5):445. doi: 10.3390/cells13050445.