PMID- 38476896
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240314
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 27
IP  - 4
DP  - 2024 Apr
TI  - Antibiotic administration aggravates asthma by disrupting gut microbiota and the 
      intestinal mucosal barrier in an asthma mouse model.
PG  - 157
LID - 10.3892/etm.2024.12445 [doi]
LID - 157
AB  - In humans, gut microbiota can determine the health status. The regulatory 
      mechanisms of the gut microbiota in asthma must be elucidated. Although 
      antibiotics (ABXs) can clear infections, they markedly alter the composition and 
      abundance of gut microbiota. The present study used ABX-treated mice to examine 
      the time-dependent effects of ABX administration on the gut microbiota and 
      intestinal mucosal barrier. The mouse asthma model was established using 
      ovalbumin (OVA) and gavaged with an ABX cocktail for different durations (1 or 2 
      weeks) and stacked sequences. The pathology of the model, model 2, OVA-ABX, 
      OVA-ABX 2, ABX-OVA and ABX-OVA was severe when compared with the control group as 
      evidenced by the following results: i) significantly increased pulmonary and 
      colonic inflammatory cell infiltration; ii) enhanced pause values and iii) 
      OVA-induced immunoglobulin E (IgE) and TGF-beta expression levels, and significantly 
      downregulated Tight Junction Protein 1 (TJP1), claudin 1 and Occludin expression 
      levels. Furthermore, the intestinal bacterial load in the OVA-ABX and OVA-ABX 2 
      groups was significantly lower than that in the ABX-OVA and ABX-OVA 2 groups, 
      respectively. The predominant taxa were as follows: phyla, Firmicutes and 
      Proteobacteria, genera, Escherichia-Shigella, Lactobacillus and Lachnospira. The 
      abundances of Lachnospira and Escherichia-Shigella were correlated with the 
      expression of OVA-induced IgE and TJPs. These findings indicated that ABX 
      administration, which modifies microbiome diversity and bacterial abundance, can 
      disrupt colonic integrity, downregulate TJ proteins, damage the intestinal 
      barrier, enhance enterocyte permeability, and promote the release of inflammatory 
      factors, adversely affecting asthma alleviation and long-term repair.
CI  - Copyright: (c) 2024 Xu et al.
FAU - Xu, Cheng-Ling
AU  - Xu CL
AD  - College of Basic Medical Sciences, Yunnan University of Traditional Chinese 
      Medicine, Kunming, Yunnan 650500, P.R. China.
FAU - Wang, Cui
AU  - Wang C
AD  - College of Basic Medical Sciences, Yunnan University of Traditional Chinese 
      Medicine, Kunming, Yunnan 650500, P.R. China.
FAU - Li, Gao-Bin
AU  - Li GB
AD  - College of Basic Medical Sciences, Yunnan University of Traditional Chinese 
      Medicine, Kunming, Yunnan 650500, P.R. China.
FAU - Zhao, Tong
AU  - Zhao T
AD  - College of Basic Medical Sciences, Yunnan University of Traditional Chinese 
      Medicine, Kunming, Yunnan 650500, P.R. China.
FAU - Zhou, Rui-Ling
AU  - Zhou RL
AD  - Department of Dermatology, First Affiliated Hospital, Yunnan University of 
      Traditional Chinese Medicine, Kunming, Yunnan 650504, P.R. China.
FAU - Chen, Jing
AU  - Chen J
AD  - College of Basic Medical Sciences, Yunnan University of Traditional Chinese 
      Medicine, Kunming, Yunnan 650500, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20240221
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC10928978
OTO - NOTNLM
OT  - asthma
OT  - inflammation
OT  - intestinal flora
OT  - tight junction proteins
COIS- The authors declare that they have no competing interests.
EDAT- 2024/03/13 06:46
MHDA- 2024/03/13 06:47
PMCR- 2024/02/21
CRDT- 2024/03/13 04:00
PHST- 2023/02/23 00:00 [received]
PHST- 2024/01/29 00:00 [accepted]
PHST- 2024/03/13 06:47 [medline]
PHST- 2024/03/13 06:46 [pubmed]
PHST- 2024/03/13 04:00 [entrez]
PHST- 2024/02/21 00:00 [pmc-release]
AID - ETM-27-4-12445 [pii]
AID - 10.3892/etm.2024.12445 [doi]
PST - epublish
SO  - Exp Ther Med. 2024 Feb 21;27(4):157. doi: 10.3892/etm.2024.12445. eCollection 
      2024 Apr.