PMID- 38478886
OWN - NLM
STAT- MEDLINE
DCOM- 20240328
LR  - 20240328
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 72
IP  - 12
DP  - 2024 Mar 27
TI  - Development of an Open Droplet Microchannel-Based Magnetosensor for 
      Immunofluorometric Assay of Trimethoprim in Chicken and Pork Samples with a Wide 
      Linear Range.
PG  - 6772-6780
LID - 10.1021/acs.jafc.4c00307 [doi]
AB  - Trimethoprim (TMP), functioning as a synergistic antibacterial agent, is utilized 
      in diagnosing and treating diseases affecting livestock and poultry. Human 
      consumption of the medication indirectly may lead to its drug accumulation in the 
      body and increase drug resistance due to its prolonged metabolic duration in 
      livestock and poultry, presenting significant health hazards. Most reported 
      immunoassay techniques, such as ELISA and immunochromatographic assay (ICA), find 
      it challenging to achieve the dual advantages of high sensitivity, simplicity of 
      operation, and a wide detection range. Consequently, an open droplet 
      microchannel-based magnetosensor for immunofluorometric assay (OMM-IFA) of 
      trimethoprim was created, featuring a gel imager to provide a signal output 
      derived from the highly specific antibody (Ab) targeting trimethoprim. The method 
      exhibited high sensitivity in chicken and pork samples, with LODs of 0.300 and 
      0.017 ng/mL, respectively, and a wide linear range, covering trimethoprim's total 
      maximum residue limits (MRLs). Additionally, the spiked recoveries in chicken and 
      pork specimens varied between 81.6% and 107.9%, maintaining an acceptable 
      variation coefficient below 15%, aligning well with the findings from the 
      ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) 
      technique. The developed method achieved a much wider linear range of about 5 
      orders of magnitude of 10(-2)-10(3) levels with grayscale signals as the output 
      signal, which exhibited high sensitivity, excellent applicability and simple 
      operability based on magnetic automation.
FAU - Wang, Lei
AU  - Wang L
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Zhang, Yongyi
AU  - Zhang Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Zeng, Dao-Ping
AU  - Zeng DP
AD  - Wens Institute, Wens Foodstuff Groups Co., Ltd., Yunfu 527499, China.
FAU - Zhu, Yuxian
AU  - Zhu Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Ling, Zhizhou
AU  - Ling Z
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Guangzhou Institute for Food Inspection, Guangzhou 510410, China.
FAU - Yang, Jinyi
AU  - Yang J
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Wang, Hong
AU  - Wang H
AUID- ORCID: 0000-0001-9136-8959
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Xu, Zhen-Lin
AU  - Xu ZL
AUID- ORCID: 0000-0002-1197-4615
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Tian, Yuanxin
AU  - Tian Y
AUID- ORCID: 0000-0003-3847-559X
AD  - Guangdong Provincial Key Laboratory of New Drug Screening, School of 
      Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
FAU - Sun, Yuanming
AU  - Sun Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Shen, Yu-Dong
AU  - Shen YD
AUID- ORCID: 0000-0001-9236-5557
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
LA  - eng
PT  - Journal Article
DEP - 20240313
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - AN164J8Y0X (Trimethoprim)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Swine
MH  - Trimethoprim
MH  - Chromatography, Liquid
MH  - Chickens
MH  - *Red Meat
MH  - *Pork Meat
MH  - Tandem Mass Spectrometry/methods
MH  - Poultry
MH  - Fluoroimmunoassay
MH  - Chromatography, High Pressure Liquid/methods
OTO - NOTNLM
OT  - immunofluorometric assay
OT  - magnetosensor
OT  - monoclonal antibody
OT  - open droplet microchannel
OT  - trimethoprim
EDAT- 2024/03/13 18:46
MHDA- 2024/03/28 06:46
CRDT- 2024/03/13 16:43
PHST- 2024/03/28 06:46 [medline]
PHST- 2024/03/13 18:46 [pubmed]
PHST- 2024/03/13 16:43 [entrez]
AID - 10.1021/acs.jafc.4c00307 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2024 Mar 27;72(12):6772-6780. doi: 10.1021/acs.jafc.4c00307. 
      Epub 2024 Mar 13.