PMID- 38481555
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240316
IS  - 2578-9430 (Electronic)
IS  - 2578-9430 (Linking)
VI  - 2024
DP  - 2024
TI  - RNA fluorescence in situ hybridization (FISH) as a method to visualize bacterial 
      colonization in the C. elegans gut.
LID - 10.17912/micropub.biology.001044 [doi]
AB  - Caenorhabditis elegans is an excellent model to study host-microbe interactions 
      as it is easy to visualize bacterial presence in their intestine. However, 
      previous studies have shown that utilizing transgenic, fluorescent 
      protein-expressing bacteria is not a reliable method to distinguish living 
      bacteria from dead bacteria in the lumen of C. elegans . In this study, we 
      compared methods for visualizing bacterial presence within the C. elegans 
      intestine and found that RNA f luorescent i n s itu h ybridization (RNA FISH) 
      could distinguish the difference between intact and dead bacteria. Thus, we 
      propose RNA FISH as the preferred method to visualize live bacterial presence in 
      the intestines of C. elegans prior to fixation.
CI  - Copyright: (c) 2024 by the authors.
FAU - Poirier, Kayla M
AU  - Poirier KM
AD  - Department of Biology, San Diego State University, San Diego, California, United 
      States.
FAU - Luallen, Robert J
AU  - Luallen RJ
AD  - Department of Biology, San Diego State University, San Diego, California, United 
      States.
FAU - Rivera, Dalaena E
AU  - Rivera DE
AD  - Department of Biology, San Diego State University, San Diego, California, United 
      States.
LA  - eng
GR  - R35 GM146836/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20240227
PL  - United States
TA  - MicroPubl Biol
JT  - microPublication biology
JID - 101759238
PMC - PMC10935869
COIS- The authors declare that there are no conflicts of interest present.
EDAT- 2024/03/14 06:46
MHDA- 2024/03/14 06:47
PMCR- 2024/02/27
CRDT- 2024/03/14 04:05
PHST- 2023/10/30 00:00 [received]
PHST- 2024/01/05 00:00 [revised]
PHST- 2024/02/25 00:00 [accepted]
PHST- 2024/03/14 06:47 [medline]
PHST- 2024/03/14 06:46 [pubmed]
PHST- 2024/03/14 04:05 [entrez]
PHST- 2024/02/27 00:00 [pmc-release]
AID - 10.17912/micropub.biology.001044 [doi]
PST - epublish
SO  - MicroPubl Biol. 2024 Feb 27;2024:10.17912/micropub.biology.001044. doi: 
      10.17912/micropub.biology.001044. eCollection 2024.