PMID- 38481895
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240315
IS  - 2168-8184 (Print)
IS  - 2168-8184 (Electronic)
IS  - 2168-8184 (Linking)
VI  - 16
IP  - 2
DP  - 2024 Feb
TI  - Studies on the Effect of Piperine on Hepatocyte Nuclear Factor 1 Alpha (HNF-1alpha) 
      and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) Levels in 
      High-Fat-Diet and Sucrose-Induced Type 2 Diabetes Mellitus Rats.
PG  - e54061
LID - 10.7759/cureus.54061 [doi]
LID - e54061
AB  - BACKGROUND: Piperine, a naturally occurring compound in black pepper (Piper 
      nigrum), is known for its potential health benefits, including its reported 
      enhancement of insulin sensitivity. However, the precise impact of piperine on 
      hepatocyte nuclear factor 1 alpha (HNF-1alpha) and sterol regulatory element-binding 
      protein 1c (SREBP-1c), transcription factors for insulin signaling and glucose 
      metabolism in hepatocytes, remains unclear. OBJECTIVE: This study aims to 
      investigate the effect of piperine, compared to metformin, on blood glucose and 
      insulin levels by modifying the expression of hepatic HNF-1alpha and SREBP-1c in 
      high-fat-diet (HFD) and sucrose-induced type 2 diabetes mellitus (T2DM) rats and 
      in human Chang liver cells. METHODS: Adult male albino rats were categorized into 
      four groups: group 1 as the control, group 2 as T2DM, group 3 as T2DM rats 
      treated with piperine (40 mg), and group 4 as T2DM rats treated with metformin 
      (50 mg). Fasting blood glucose (FBG) and serum insulin levels were measured using 
      enzyme-linked immunosorbent assay (ELISA), while real-time polymerase chain 
      reaction (RT-PCR) analysis was conducted to assess the mRNA expression of HNF-1alpha 
      and SREBP-1c. Further, piperine was treated with normal and high glucose-induced 
      Chang liver cells, and gene expression was analysed. Data analysis was performed 
      using one-way analysis of variance (ANOVA), with a significance set at p<0.05. 
      RESULTS: Treatment with piperine led to a notable decrease in blood glucose 
      levels and circulating insulin when compared with T2DM rats (group 2). 
      Additionally, piperine administration resulted in the upregulation of HNF-1alpha mRNA 
      expression and downregulation of SREBP-1c mRNA levels whose effects were found to 
      be near that of the control and standard drug metformin's effects. In vitro study 
      also confirmed that piperine improved the HNF-1alpha expression and reduced the 
      expression of SREBP-1c in Chang liver cells. CONCLUSION: Our findings suggest 
      that piperine treatment effectively regulates hyperglycemic and hyperinsulinemic 
      insulin resistance in the liver by modulating the expression of HNF-1alpha and 
      SREBP-1c. Consequently, piperine emerges as a promising candidate for therapeutic 
      intervention in managing T2DM.
CI  - Copyright (c) 2024, Zuvairiya et al.
FAU - Zuvairiya, Ummu
AU  - Zuvairiya U
AD  - Centre of Molecular Medicine and Diagnostics (COMManD), Department of 
      Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of 
      Medical and Technical Sciences, Saveetha University, Chennai, IND.
FAU - Jayaraman, Selvaraj
AU  - Jayaraman S
AD  - Centre of Molecular Medicine and Diagnostics (COMManD), Department of 
      Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of 
      Medical and Technical Sciences, Saveetha University, Chennai, IND.
FAU - Sankaran, Kavitha
AU  - Sankaran K
AD  - Centre of Molecular Medicine and Diagnostics (COMManD), Department of 
      Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of 
      Medical and Technical Sciences, Saveetha University, Chennai, IND.
FAU - Veeraraghavan, Vishnu Priya
AU  - Veeraraghavan VP
AD  - Centre of Molecular Medicine and Diagnostics (COMManD), Department of 
      Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of 
      Medical and Technical Sciences, Saveetha University, Chennai, IND.
FAU - R, Gayathri
AU  - R G
AD  - Centre of Molecular Medicine and Diagnostics (COMManD), Department of 
      Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of 
      Medical and Technical Sciences, Saveetha University, Chennai, IND.
LA  - eng
PT  - Journal Article
DEP - 20240212
PL  - United States
TA  - Cureus
JT  - Cureus
JID - 101596737
PMC - PMC10933888
OTO - NOTNLM
OT  - black pepper
OT  - gene expression
OT  - high-fat diet
OT  - insulin resistance
OT  - male wistar rat
COIS- The authors have declared that no competing interests exist.
EDAT- 2024/03/14 06:46
MHDA- 2024/03/14 06:47
PMCR- 2024/02/12
CRDT- 2024/03/14 04:16
PHST- 2023/08/20 00:00 [received]
PHST- 2024/02/10 00:00 [accepted]
PHST- 2024/03/14 06:47 [medline]
PHST- 2024/03/14 06:46 [pubmed]
PHST- 2024/03/14 04:16 [entrez]
PHST- 2024/02/12 00:00 [pmc-release]
AID - 10.7759/cureus.54061 [doi]
PST - epublish
SO  - Cureus. 2024 Feb 12;16(2):e54061. doi: 10.7759/cureus.54061. eCollection 2024 
      Feb.