PMID- 38483612
OWN - NLM
STAT- MEDLINE
DCOM- 20240417
LR  - 20240426
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 484
IP  - 3
DP  - 2024 Mar
TI  - The diagnostic utility of cytology specimen in a case of EWSR1::NFATC2 sarcoma.
PG  - 533-538
LID - 10.1007/s00428-024-03784-x [doi]
AB  - EWSR1::NFATC2 sarcoma, a rare round cell sarcoma constituting the majority of 
      EWSR1::non-ETS sarcomas, has recently been defined in the latest WHO 
      classification. To date, the cytological findings of EWSR1::NFATC2 sarcoma remain 
      undocumented. We present the case of a 25-year-old man with a history of 
      polyostotic fibrous dysplasia in the right leg, referred to our hospital with 
      left thigh pain. Cytological findings included metachromasia, minimally 
      pleomorphic round cells, and eosinophilic infiltration. There was no precursor 
      fibrous dysplasia and the initial diagnosis was undifferentiated pleomorphic 
      sarcoma. Following histologic review, we successfully performed 
      immunocytochemistry and fluorescence in situ hybridization (FISH) on archival 
      cytology specimens. The tumor cells were positive for NKX2-2, NKX3-1, and PAX7 
      and showed amplified 5' single signals of EWSR1 gene. Reverse 
      transcriptase-polymerase chain reaction revealed an in-frame fusion of EWSR1 and 
      NFATC2. This report describes the cytological features of EWSR1::NFATC2 sarcoma 
      and highlights the diagnostic utility of archival cytology specimens.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Takeda, Naoki
AU  - Takeda N
AUID- ORCID: 0009-0008-2362-7179
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Makise, Naohiro
AU  - Makise N
AUID- ORCID: 0000-0002-6991-747X
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan. makise58@gmail.com.
FAU - Kageyama, Hajime
AU  - Kageyama H
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Takahashi, Tsukasa
AU  - Takahashi T
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Katoh, Hiroshi
AU  - Katoh H
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Odaka, Akiko
AU  - Odaka A
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Oikawa, Mariko
AU  - Oikawa M
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Sugiyama, Takahiro
AU  - Sugiyama T
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Kawana, Hidetada
AU  - Kawana H
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Araki, Akinobu
AU  - Araki A
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
FAU - Kinoshita, Hideyuki
AU  - Kinoshita H
AD  - Division of Orthopaedic Surgery, Chiba Cancer Center, Chiba, Japan.
FAU - Hagiwara, Yoko
AU  - Hagiwara Y
AD  - Division of Orthopaedic Surgery, Chiba Cancer Center, Chiba, Japan.
FAU - Kamoda, Hiroto
AU  - Kamoda H
AD  - Division of Orthopaedic Surgery, Chiba Cancer Center, Chiba, Japan.
FAU - Yonemoto, Tsukasa
AU  - Yonemoto T
AD  - Division of Orthopaedic Surgery, Chiba Cancer Center, Chiba, Japan.
FAU - Itami, Makiko
AU  - Itami M
AD  - Division of Surgical Pathology, Chiba Cancer Center, 666-2 Nitona-Cho, Chuo-Ku, 
      Chiba-Shi, Chiba, 260-8717, Japan.
LA  - eng
GR  - JP23K06438/JSPS KAKENHI/
PT  - Case Reports
PT  - Journal Article
DEP - 20240314
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (EWSR1 protein, human)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (NFATC2 protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Transcription Factors)
RN  - 0 (NFATc2-EWSR1 fusion protein, human)
SB  - IM
MH  - Adult
MH  - Humans
MH  - Male
MH  - *Cytology
MH  - Diagnosis, Differential
MH  - In Situ Hybridization, Fluorescence
MH  - NFATC Transcription Factors/genetics
MH  - *Oncogene Proteins, Fusion/genetics
MH  - RNA-Binding Protein EWS/genetics
MH  - *Sarcoma/diagnosis/genetics
MH  - Transcription Factors/genetics
OTO - NOTNLM
OT  - EWSR1::NFATC2 sarcoma
OT  - Cytology
OT  - Eosinophil
OT  - FISH
OT  - Immunocytochemistry
OT  - Metachromasia
EDAT- 2024/03/14 18:42
MHDA- 2024/04/17 06:42
CRDT- 2024/03/14 12:17
PHST- 2023/12/27 00:00 [received]
PHST- 2024/03/10 00:00 [accepted]
PHST- 2024/02/26 00:00 [revised]
PHST- 2024/04/17 06:42 [medline]
PHST- 2024/03/14 18:42 [pubmed]
PHST- 2024/03/14 12:17 [entrez]
AID - 10.1007/s00428-024-03784-x [pii]
AID - 10.1007/s00428-024-03784-x [doi]
PST - ppublish
SO  - Virchows Arch. 2024 Mar;484(3):533-538. doi: 10.1007/s00428-024-03784-x. Epub 
      2024 Mar 14.