PMID- 38485913
OWN - NLM
STAT- MEDLINE
DCOM- 20240318
LR  - 20240318
IS  - 1735-5249 (Electronic)
IS  - 1735-1502 (Linking)
VI  - 23
IP  - 1
DP  - 2024 Feb 11
TI  - Exosomes Derived from Heat-shocked Tumor Cells Promote In vitro Maturation of 
      Bone Marrow-derived Dendritic Cells.
PG  - 97-106
LID - 10.18502/ijaai.v23i1.14957 [doi]
AB  - Dendritic cells (DCs), professional antigen-presenting cells that process and 
      deliver antigens using MHC II/I molecules, can be enhanced in numerous ways.  
      Exosomes derived from heat-shocked tumor cells (HS-TEXs) contain high amounts of 
      heat-shock proteins (HSPs). HSPs, as chaperons, can induce DC maturation. This 
      study aimed to investigate whether HS-TEXs can promote DC maturation. To generate 
      DC, bone marrow-derived cells were treated with Interleukin-4 and GM-CSF. 
      Exosomes were isolated from heat-treated CT-26 cells. The expression level of HSP 
      in exosomes was checked by western blot and the increase in the expression of 
      this protein was observed. Then, HS-TEXs were co-cultured with iDCs to determine 
      DC maturity, and then DCs were co-cultured with lymphocytes to determine DC 
      activity. Our results showed that  DCs treated with HS-TEXs express high levels 
      of molecules involved in DC maturation and function including MHCII, CD40, CD83, 
      and CD86. HS-TEXs caused phenotypic and functional maturation of DCs. In 
      addition, flow cytometric results reflected a higher proliferative response of 
      lymphocytes in the iDC / Tex + HSP group. HS-TEXs could be used as a strategy to 
      improve DC maturation and activation.
FAU - Heidari, Neda
AU  - Heidari N
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. nh.he21@yahoo.com.
FAU - Abbasi-Kenarsari, Hajar
AU  - Abbasi-Kenarsari H
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. hajar.abbasi64@yahoo.com.
FAU - Niknam, Bahare
AU  - Niknam B
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. bahare.niknam@yahoo.com.
FAU - Asadirad, Ali
AU  - Asadirad A
AD  - Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of 
      Medical Sciences, Ahvaz, Iran. ali.asadirad@gmail.com.
FAU - Amani, Davar
AU  - Amani D
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. Amanid@sbmu.ac.ir.
FAU - Mirsanei, Zahra
AU  - Mirsanei Z
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. sz.mirsanei@gmail.com.
FAU - Hashemi, Seyed Mahmood
AU  - Hashemi SM
AD  - Department of Immunology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran AND Medical Nanotechnology and Tissue Engineering 
      Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 
      smmhashemi@sbmu.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20240211
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
SB  - IM
MH  - *Exosomes
MH  - Dendritic Cells
MH  - Bone Marrow
MH  - T-Lymphocytes
MH  - Coculture Techniques
MH  - Cell Differentiation
OTO - NOTNLM
OT  - Dendritic cell
OT  - Exosome
OT  - Heat-shock protein
OT  - Tumor
EDAT- 2024/03/15 06:43
MHDA- 2024/03/18 06:43
CRDT- 2024/03/15 00:25
PHST- 2023/03/09 00:00 [received]
PHST- 2023/11/24 00:00 [accepted]
PHST- 2024/03/18 06:43 [medline]
PHST- 2024/03/15 06:43 [pubmed]
PHST- 2024/03/15 00:25 [entrez]
AID - 10.18502/ijaai.v23i1.14957 [doi]
PST - epublish
SO  - Iran J Allergy Asthma Immunol. 2024 Feb 11;23(1):97-106. doi: 
      10.18502/ijaai.v23i1.14957.