PMID- 38488886 OWN - NLM STAT- MEDLINE DCOM- 20240426 LR - 20240429 IS - 1433-3023 (Electronic) IS - 0937-3462 (Print) IS - 0937-3462 (Linking) VI - 35 IP - 4 DP - 2024 Apr TI - Effect of Vaginal Microecological Alterations on Female Pelvic Organ Prolapse. PG - 881-891 LID - 10.1007/s00192-024-05759-7 [doi] AB - INTRODUCTION AND HYPOTHESIS: The objective was to investigate the correlation between endogenous vaginal microecological alterations and female pelvic organ prolapse (POP). METHODS: Patients who underwent vaginal hysterectomy were retrospectively analyzed as the POP group (n = 30) and the non-POP group (n = 30). The vaginal microbial metabolites and enzyme levels were tested using the dry chemoenzymatic method. The mRNA and protein expression were tested using real-time quantitative PCR and immunohistochemistry. SPSS version 25.0 and GraphPad Prism 8.0 were performed for statistical analysis. RESULTS: Compared with the non-POP group, the vaginal pH, H(2)O(2) positivity and leukocyte esterase positivity were higher in patients with POP (all p < 0.05). Further analysis showed that patients with pelvic organ prolapse quantification (POP-Q) stage IV had higher rates of vaginal pH, H(2)O(2) positivity and leukocyte esterase positivity than those with POP-Q stage III. Additionally, the mRNA expression of decorin (DCN), transforming growth factor beta 1 (TGF-beta1), and matrix metalloproteinase-3 (MMP-3) in uterosacral ligament tissues were higher, whereas collagen I and III were lower. Similarly, the positive expression of MMP-3 in uterosacral ligament tissue was significantly upregulated in the POP group compared with the non-POP group (p = 0.035), whereas collagen I (p = 0.004) and collagen III (p = 0.019) in uterosacral ligament tissue were significantly downregulated in the POP group. Correlation analysis revealed that there was a significant correlation between vaginal microecology and collagen metabolism. In addition, MMP-3 correlated negatively with collagen I and collagen III (p = 0.002, r = -0.533; p = 0.002, r = -0.534 respectively), whereas collagen I correlated positively with collagen III (p = 0.001, r = 0.578). CONCLUSIONS: Vaginal microecological dysbiosis affects the occurrence of female POP, which could be considered a novel therapeutic option. CI - (c) 2024. The Author(s). FAU - Chen, Shaozhan AU - Chen S AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Zheng, Qiaomei AU - Zheng Q AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Zhang, Limin AU - Zhang L AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Chen, Lihong AU - Chen L AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. wscclh@163.com. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. wscclh@163.com. FAU - Wang, Jinhua AU - Wang J AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. jinhuawang126@126.com. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. jinhuawang126@126.com. LA - eng GR - 2019QH1103/Startup Fund for Scientific Research of Fujian Medical University/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20240315 PL - England TA - Int Urogynecol J JT - International urogynecology journal JID - 101567041 RN - EC 3.4.24.17 (Matrix Metalloproteinase 3) RN - 0 (Decorin) RN - 0 (Transforming Growth Factor beta1) RN - BBX060AN9V (Hydrogen Peroxide) RN - 0 (Collagen Type I) RN - 0 (Collagen Type III) RN - 0 (RNA, Messenger) SB - IM MH - Female MH - Humans MH - *Pelvic Organ Prolapse/metabolism MH - *Vagina MH - Middle Aged MH - Retrospective Studies MH - Matrix Metalloproteinase 3/metabolism MH - Decorin/metabolism/genetics MH - Aged MH - Transforming Growth Factor beta1/metabolism/genetics MH - Hydrogen Peroxide/metabolism MH - Hydrogen-Ion Concentration MH - Hysterectomy, Vaginal MH - Collagen Type I/metabolism/genetics MH - Collagen Type III/metabolism/genetics MH - RNA, Messenger/metabolism MH - Ligaments/metabolism MH - Microbiota MH - Adult PMC - PMC11052768 OTO - NOTNLM OT - Collagen I OT - Collagen III OT - Matrix metalloproteinase-3 OT - Pelvic organ prolapse OT - Vaginal microbiome COIS- None. EDAT- 2024/03/15 18:42 MHDA- 2024/04/26 13:26 PMCR- 2024/03/15 CRDT- 2024/03/15 12:04 PHST- 2023/10/18 00:00 [received] PHST- 2024/02/04 00:00 [accepted] PHST- 2024/04/26 13:26 [medline] PHST- 2024/03/15 18:42 [pubmed] PHST- 2024/03/15 12:04 [entrez] PHST- 2024/03/15 00:00 [pmc-release] AID - 10.1007/s00192-024-05759-7 [pii] AID - 5759 [pii] AID - 10.1007/s00192-024-05759-7 [doi] PST - ppublish SO - Int Urogynecol J. 2024 Apr;35(4):881-891. doi: 10.1007/s00192-024-05759-7. Epub 2024 Mar 15.