PMID- 38489968
OWN - NLM
STAT- MEDLINE
DCOM- 20240401
LR  - 20240401
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 254
DP  - 2024 Jun 15
TI  - A point-of-care electrochemical biosensor for the rapid and sensitive detection 
      of biomarkers in murine models with LPS-induced sepsis.
PG  - 116202
LID - S0956-5663(24)00207-0 [pii]
LID - 10.1016/j.bios.2024.116202 [doi]
AB  - Sepsis is a life-threatening condition, which is irreversible if diagnosis and 
      intervention are delayed. The response of the immune cells towards an infection 
      triggers widespread inflammation through the production of cytokines, which may 
      result in multiple organ dysfunction and eventual death. Conventional detection 
      techniques fail to provide a rapid diagnosis because of their limited sensitivity 
      and tedious protocol. This study proposes a point-of-care (POC) electrochemical 
      biosensor that overcomes the limitations of current biosensing technologies in 
      the clinical setting by its integration with electrokinetics, enhancing the 
      sensitivity to picogram level compared with the nanogram limit of current 
      diagnostic technologies. This biosensor promotes the use of a microelectrode 
      strip to address the limitations of conventional photolithographic fabrication 
      methods. Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and 
      microRNA-155 (miR-155) were monitored in a lipopolysaccharide (LPS)-induced 
      septic mouse model. The optimum target hybridization time in a high conductivity 
      medium was observed to be 60 s leading to the completion of the whole operation 
      within 5 min compared with the 4-h detection time of the traditional 
      enzyme-linked immunosorbent assay (ELISA). The limit of detection (LOD) was 
      calculated to be 0.84, 0.18, and 0.0014 pg mL(-1), respectively. This novel 
      sensor may have potential for the early diagnosis of sepsis in the clinical 
      setting.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Ondevilla, Neil Adrian P
AU  - Ondevilla NAP
AD  - Department of Biomedical Engineering, National Cheng Kung University, Tainan, 
      70101, Taiwan.
FAU - Liu, Peng-Wen
AU  - Liu PW
AD  - Department of Biomedical Engineering, National Cheng Kung University, Tainan, 
      70101, Taiwan.
FAU - Huang, Wan-Ting
AU  - Huang WT
AD  - Department of Dermatology, National Cheng Kung University Hospital, College of 
      Medicine, National Cheng Kung University, Tainan, 70430, Taiwan.
FAU - Weng, Tzu-Ping
AU  - Weng TP
AD  - Division of Infectious Diseases, Department of Internal Medicine and Center for 
      Infection Control, National Cheng Kung University Hospital, College of Medicine, 
      National Cheng Kung University, Tainan, 70101, Taiwan.
FAU - Lee, Nan-Yao
AU  - Lee NY
AD  - Division of Infectious Diseases, Department of Internal Medicine and Center for 
      Infection Control, National Cheng Kung University Hospital, College of Medicine, 
      National Cheng Kung University, Tainan, 70101, Taiwan.
FAU - Ma, Syu-Cing
AU  - Ma SC
AD  - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 
      Taipei, 106, Taiwan.
FAU - Huang, Jian-Jang
AU  - Huang JJ
AD  - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 
      Taipei, 106, Taiwan; Department of Electrical Engineering, National Taiwan 
      University, Taipei, 106, Taiwan.
FAU - Wong, Tak-Wah
AU  - Wong TW
AD  - Department of Dermatology, National Cheng Kung University Hospital, College of 
      Medicine, National Cheng Kung University, Tainan, 70430, Taiwan; Department of 
      Biochemistry & Molecular Biology, College of Medicine, National Cheng Kung 
      University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng 
      Kung University, Tainan, 70101, Taiwan. Electronic address: 
      dr.kentwwong@gmail.com.
FAU - Chang, Hsien-Chang
AU  - Chang HC
AD  - Department of Biomedical Engineering, National Cheng Kung University, Tainan, 
      70101, Taiwan; Medical Device Innovation Center, National Cheng Kung University, 
      Tainan, 70101, Taiwan. Electronic address: hcchang@mail.ncku.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20240310
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Biomarkers)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Lipopolysaccharides/toxicity
MH  - Point-of-Care Systems
MH  - Disease Models, Animal
MH  - *Biosensing Techniques/methods
MH  - *Sepsis/chemically induced/diagnosis
MH  - Biomarkers/analysis
MH  - Tumor Necrosis Factor-alpha
MH  - *MicroRNAs/analysis
OTO - NOTNLM
OT  - DC-Biased AC electrokinetics
OT  - Electrochemical biosensor
OT  - POC
OT  - cytokines
OT  - miR-155
OT  - sepsis
COIS- Declaration of competing interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: Hsien-Chang Chang, Neil Adrian P. Ondevilla, Peng-Wen Liu has patent 
      #TWI803097B issued to National Cheng Kung University.
EDAT- 2024/03/16 05:42
MHDA- 2024/04/01 06:43
CRDT- 2024/03/15 19:07
PHST- 2024/01/18 00:00 [received]
PHST- 2024/02/26 00:00 [revised]
PHST- 2024/03/08 00:00 [accepted]
PHST- 2024/04/01 06:43 [medline]
PHST- 2024/03/16 05:42 [pubmed]
PHST- 2024/03/15 19:07 [entrez]
AID - S0956-5663(24)00207-0 [pii]
AID - 10.1016/j.bios.2024.116202 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2024 Jun 15;254:116202. doi: 10.1016/j.bios.2024.116202. 
      Epub 2024 Mar 10.