PMID- 38491617
OWN - NLM
STAT- MEDLINE
DCOM- 20240318
LR  - 20240318
IS  - 2470-0053 (Electronic)
IS  - 2470-0045 (Linking)
VI  - 109
IP  - 2-1
DP  - 2024 Feb
TI  - Chromatin structure from high resolution microscopy: Scaling laws and microphase 
      separation.
PG  - 024408
LID - 10.1103/PhysRevE.109.024408 [doi]
AB  - Recent advances in experimental fluorescence microscopy allow high accuracy 
      determination (resolution of 50 nm) of the three-dimensional physical location of 
      multiple (up to  approximately 10;2) tagged regions of the chromosome. We investigate 
      publicly available microscopy data for two loci of the human Chr21 obtained from 
      multiplexed fluorescence in situ hybridization (FISH) methods for different cell 
      lines and treatments. Inspired by polymer physics models, our analysis centers 
      around distance distributions between different tags with the aim being to 
      unravel the chromatin conformational arrangements. We show that for any specific 
      genomic site, there are (at least) two different conformational arrangements of 
      chromatin, implying coexisting distinct topologies which we refer to as phase alpha 
      and phase beta. These two phases show different scaling behaviors: the former is 
      consistent with a crumpled globule, while the latter indicates a confined, but 
      more extended conformation, such as a looped domain. The identification of these 
      distinct phases sheds light on the coexistence of multiple chromatin topologies 
      and provides insights into the effects of cellular context and/or treatments on 
      chromatin structure.
FAU - Remini, Loucif
AU  - Remini L
AD  - Laboratoire Charles Coulomb (L2C), Universite de Montpellier, CNRS UMR5221, 
      Montpellier, France.
FAU - Segers, Midas
AU  - Segers M
AD  - Soft Matter and Biophysics, KU Leuven, Celestijnenlaan 200D, 3001 Leuven, 
      Belgium.
FAU - Palmeri, John
AU  - Palmeri J
AD  - Laboratoire Charles Coulomb (L2C), Universite de Montpellier, CNRS UMR5221, 
      Montpellier, France.
FAU - Walter, Jean-Charles
AU  - Walter JC
AD  - Laboratoire Charles Coulomb (L2C), Universite de Montpellier, CNRS UMR5221, 
      Montpellier, France.
FAU - Parmeggiani, Andrea
AU  - Parmeggiani A
AD  - Laboratoire Charles Coulomb (L2C), Universite de Montpellier, CNRS UMR5221, 
      Montpellier, France.
FAU - Carlon, Enrico
AU  - Carlon E
AD  - Soft Matter and Biophysics, KU Leuven, Celestijnenlaan 200D, 3001 Leuven, 
      Belgium.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Phys Rev E
JT  - Physical review. E
JID - 101676019
RN  - 0 (Chromatin)
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Chromatin
MH  - *Chromosomes
MH  - Genome
MH  - Microscopy, Fluorescence/methods
EDAT- 2024/03/16 21:43
MHDA- 2024/03/18 06:43
CRDT- 2024/03/16 01:01
PHST- 2023/08/14 00:00 [received]
PHST- 2024/01/11 00:00 [accepted]
PHST- 2024/03/18 06:43 [medline]
PHST- 2024/03/16 21:43 [pubmed]
PHST- 2024/03/16 01:01 [entrez]
AID - 10.1103/PhysRevE.109.024408 [doi]
PST - ppublish
SO  - Phys Rev E. 2024 Feb;109(2-1):024408. doi: 10.1103/PhysRevE.109.024408.