PMID- 38492186
OWN - NLM
STAT- Publisher
LR  - 20240316
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
DP  - 2024 Mar 16
TI  - Immunomodulatory and Anti-Inflammatory Effects of Vitamin A and Tryptophan on 
      Monocyte-Derived Dendritic Cells Stimulated with Gliadin in Celiac Disease 
      Patients.
LID - 10.1007/s10753-024-02004-7 [doi]
AB  - Celiac Disease (CeD) is an autoimmune disorder with various symptoms upon gluten 
      exposure. Dendritic cells (DCs) play a crucial role in gliadin-induced 
      inflammation. Vitamin A (retinol; Ret) and its metabolite, retinoic acid (RA), 
      along with tryptophan (Trp) and its metabolite, kynurenic acid (KYNA), are known 
      to influence the immune function of DCs and enhance their tolerogenicity. This 
      research aims to assess the impact of gliadin on DC maturation and the potential 
      of vitamin A and tryptophan to induce immune tolerance in DCs. The monocyte cells 
      obtained from peripheral blood mononuclear cells (PBMCs) of celiac disease 
      patients were differentiated into DCs in the absence or presence of Ret, RA, Trp, 
      KYNA, and then stimulated with peptic and tryptic (PT) digested of gliadin. We 
      used flow cytometry to analyze CD11c, CD14, HLA-DR, CD83, CD86, and CD103 
      expression. ELISA was carried out to measure TGF-beta, IL-10, IL-12, and TNF-alpha 
      levels. qRT-PCR was used to assess the mRNA expression of retinaldehyde 
      dehydrogenase 2 (RALDH2) and integrin alphaE (CD103). The mRNA and protein levels of 
      Indoleamine 2, 3-dioxygenase (IDO) was analyzed by qRT-PCR and Western blot 
      assays, respectively. Our findings demonstrate that PT-gliadin enhances the 
      expression of maturation markers, i.e. CD83, CD86 and HLA-DR and promote the 
      secretion of TNF-alpha and IL-12 in DCs. Interestingly, vitamin A, tryptophan, and 
      their metabolites increase the expression of CD103, while limiting the expression 
      of HLA-DR, CD83, and CD86. They also enhance RALDH2 and IDO expression and 
      promote the secretion of TGF-beta and IL-10, while limiting IL-12 and TNF-alpha 
      secretion. These findings suggest that vitamin A and tryptophan have beneficial 
      effects on PT-gliadin-stimulated DCs, highlighting their potential as therapeutic 
      agents for celiac disease. However, further research is needed to fully 
      understand their underlying mechanisms of action in these cells.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Asgari, Fatemeh
AU  - Asgari F
AD  - Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
AD  - Gastroenterology and Liver Diseases Research Center, Research Institute for 
      Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical 
      Sciences, Tehran, Iran.
FAU - Nikzamir, Abdolrahim
AU  - Nikzamir A
AD  - Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Baghaei, Kaveh
AU  - Baghaei K
AD  - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, 
      Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Salami, Siamak
AU  - Salami S
AD  - Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Masotti, Andrea
AU  - Masotti A
AD  - Bambino Gesu Children's Hospital-IRCCS, Research Laboratories, V.le San Paolo 15, 
      00146, Rome, Italy.
FAU - Rostami-Nejad, Mohammad
AU  - Rostami-Nejad M
AD  - Celiac Disease and Gluten Related Disorders Research Center, Research Institute 
      for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical 
      Sciences, Tehran, Iran. m.rostamii@sbmu.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20240316
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
SB  - IM
OTO - NOTNLM
OT  - 3-dioxygenase
OT  - Celiac disease
OT  - Dendritic cells
OT  - Immune tolerance.
OT  - Indoleamine 2
OT  - Tryptophan
OT  - Vitamin A
EDAT- 2024/03/16 21:47
MHDA- 2024/03/16 21:47
CRDT- 2024/03/16 12:21
PHST- 2023/12/12 00:00 [received]
PHST- 2024/03/04 00:00 [accepted]
PHST- 2024/02/29 00:00 [revised]
PHST- 2024/03/16 21:47 [medline]
PHST- 2024/03/16 21:47 [pubmed]
PHST- 2024/03/16 12:21 [entrez]
AID - 10.1007/s10753-024-02004-7 [pii]
AID - 10.1007/s10753-024-02004-7 [doi]
PST - aheadofprint
SO  - Inflammation. 2024 Mar 16. doi: 10.1007/s10753-024-02004-7.