PMID- 38492337 OWN - NLM STAT- MEDLINE DCOM- 20240410 LR - 20240410 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 131 DP - 2024 Apr 20 TI - Fucosterol isolated from Sargassum horneri attenuates allergic responses in immunoglobulin E/bovine serum albumin-stimulated mast cells and passive cutaneous anaphylaxis in mice. PG - 111851 LID - S1567-5769(24)00369-2 [pii] LID - 10.1016/j.intimp.2024.111851 [doi] AB - Allergic diseases have become a serious problem worldwide and occur when the immune system overreacts to stimuli. Sargassum horneri is an edible marine brown alga with pharmacological relevance in treating various allergy-related conditions. Therefore, this study aimed to investigate the effect of fucosterol (FST) isolated from S. horneri on immunoglobulin E(IgE)/bovine serum albumin (BSA)-stimulated allergic reactions in mouse bone marrow-derived cultured mast cells (BMCMCs) and passive cutaneous anaphylaxis (PCA) in BALB/c mice. The in silico analysis results revealed the binding site modulatory potential of FST on the IgE and IgE-FcepsilonRI complex. The findings of the study revealed that FST significantly suppressed the degranulation of IgE/BSA-stimulated BMCMCs by inhibiting the release of beta-hexosaminidase and histamine in a dose-dependent manner. In addition, FST effectively decreased the expression of FcepsilonRI on the surface of BMCMCs and its IgE binding. FST dose-dependently downregulated the expression of allergy-related cytokines (interleukin (IL)-4, -5, -6, -13, tumor necrosis factor (TNF)-alpha, and a chemokine (thymus and activation-regulated chemokine (TARC)) by suppressing the activation of nuclear factor-kappaB (NF-kappaB) and Syk-LAT-ERK-Gab2 signaling in IgE/BSA-stimulated BMCMCs. As per the histological analysis results of the in vivo studies with IgE-mediated PCA in BALB/c mice, FST treatment effectively attenuated the PCA reactions. These findings suggest that FST has an immunopharmacological potential as a naturally available bioactive compound for treating allergic reactions. CI - Copyright (c) 2024 Elsevier B.V. All rights reserved. FAU - Maheshika Kumari Jayasinghe, Arachchige AU - Maheshika Kumari Jayasinghe A AD - Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of Korea. Electronic address: 218385@jnu.ac.kr. FAU - Yang, Hye-Won AU - Yang HW AD - Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: koty221@naver.com. FAU - Gedara Isuru Sandanuwan Kirindage, Kirinde AU - Gedara Isuru Sandanuwan Kirindage K AD - Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of Korea. Electronic address: 218388@jnu.ac.kr. FAU - Jung, Kyungsook AU - Jung K AD - Functional Biomaterials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, Republic of Korea. Electronic address: jungks@kribb.re.kr. FAU - Je, Jun-Geon AU - Je JG AD - Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: wpwnsrjs@naver.com. FAU - Wang, Lei AU - Wang L AD - College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: leiwang2021@ouc.edu.cn. FAU - Kim, Kil-Nam AU - Kim KN AD - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea. Electronic address: knkim@kbsi.re.kr. FAU - Ahn, Ginnae AU - Ahn G AD - Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of Korea; Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Republic of Korea. Electronic address: gnahn@jnu.ac.kr. LA - eng PT - Journal Article DEP - 20240315 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 RN - 37341-29-0 (Immunoglobulin E) RN - 27432CM55Q (Serum Albumin, Bovine) RN - 504ZAM710C (fucosterol) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Anti-Allergic Agents) RN - 99WUK5D0Y8 (Stigmasterol) SB - IM MH - Mice MH - Animals MH - Immunoglobulin E/metabolism MH - Serum Albumin, Bovine MH - *Sargassum/metabolism MH - Mast Cells MH - Passive Cutaneous Anaphylaxis MH - *Hypersensitivity/drug therapy MH - Tumor Necrosis Factor-alpha/metabolism MH - *Anaphylaxis MH - Cell Degranulation MH - Mice, Inbred BALB C MH - *Anti-Allergic Agents/pharmacology/therapeutic use MH - Stigmasterol/*analogs & derivatives OTO - NOTNLM OT - Anti-allergic effect OT - Fucosterol OT - Mouse bone marrow-derived cultured mast cells OT - Passive cutaneous anaphylaxis OT - Sargassum horneri COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/03/17 00:42 MHDA- 2024/04/10 06:43 CRDT- 2024/03/16 19:05 PHST- 2023/11/23 00:00 [received] PHST- 2024/02/29 00:00 [revised] PHST- 2024/03/10 00:00 [accepted] PHST- 2024/04/10 06:43 [medline] PHST- 2024/03/17 00:42 [pubmed] PHST- 2024/03/16 19:05 [entrez] AID - S1567-5769(24)00369-2 [pii] AID - 10.1016/j.intimp.2024.111851 [doi] PST - ppublish SO - Int Immunopharmacol. 2024 Apr 20;131:111851. doi: 10.1016/j.intimp.2024.111851. Epub 2024 Mar 15.