PMID- 38493623
OWN - NLM
STAT- MEDLINE
DCOM- 20240408
LR  - 20240408
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 469
DP  - 2024 May 5
TI  - Biotransformation of HBCDs by the microbial communities enriched from mangrove 
      sediments.
PG  - 134036
LID - S0304-3894(24)00615-0 [pii]
LID - 10.1016/j.jhazmat.2024.134036 [doi]
AB  - 1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs) are a sort of persistent organic 
      pollutants (POPs). This research investigated 12 microbial communities enriched 
      from sediments of four mangroves in China to transform HBCDs. Six microbial 
      communities gained high transformation rates (27.5-97.7%) after 12 generations of 
      serial transfer. Bacteria were the main contributors to transform HBCDs rather 
      than fungi. Analyses on the bacterial compositions and binning genomes showed 
      that Alcanivorax (55.246-84.942%) harboring haloalkane dehalogenase genes dadAH 
      and dadBH dominated the microbial communities with high transformation rates. 
      Moreover, expressions of dadAH and dadBH in the microbial communities and 
      Alcanivorax isolate could be induced by HBCDs. Further, it was found that 
      purified proteins DadAH and DadBH showed high conversion rates on HBCDs in 36 h 
      (91.9 +/- 7.4 and 101.0 +/- 1.8%, respectively). The engineered Escherichia coli BL21 
      strains harbored two genes could convert 5.7 +/- 0.4 and 35.1 +/- 0.1% HBCDs, 
      respectively, lower than their cell-free crude extracts (61.2 +/- 5.2 and 
      56.5 +/- 8.7%, respectively). The diastereoisomer-specific transforming trend by 
      both microbial communities and enzymes were gamma- > alpha- > beta-HBCD, differed from alpha- 
      > beta- > gamma-HBCD by the Alcanivorax isolate. The identified transformation products 
      indicated that HBCDs were dehalogenated via HBr elimination (dehydrobromination), 
      hydrolytic and reductive debromination pathways in the enriched cultures. Two 
      enzymes converted HBCDs via hydrolytic debromination. The present research 
      provided theoretical bases for the biotransformation of HBCDs by microbial 
      community and the bioremediation of HBCDs contamination in the environment.
CI  - Copyright (c) 2024. Published by Elsevier B.V.
FAU - Yu, Fei
AU  - Yu F
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Zhang, Bing
AU  - Zhang B
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Liu, Yongjin
AU  - Liu Y
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Luo, Wenqi
AU  - Luo W
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Chen, Haonan
AU  - Chen H
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Gao, Jun'na
AU  - Gao J
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Ye, Xueying
AU  - Ye X
AD  - School of Life Sciences, Huizhou University, Huizhou 516007, Guangdong Province, 
      China.
FAU - Li, Jin
AU  - Li J
AD  - Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, 
      Shantou University, Shantou 515063, Guangdong Province, China.
FAU - Xie, Qingyi
AU  - Xie Q
AD  - Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Peng, Tao
AU  - Peng T
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Huang, Tongwang
AU  - Huang T
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China.
FAU - Hu, Zhong
AU  - Hu Z
AD  - Department of Biology, College of Science, Shantou University, Shantou 515063, 
      Guangdong Province, China. Electronic address: hzh@stu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240313
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 5I9835JO3M (hexabromocyclododecane)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Flame Retardants)
SB  - IM
MH  - Stereoisomerism
MH  - *Hydrocarbons, Brominated/metabolism
MH  - Biotransformation
MH  - Bacteria/metabolism
MH  - *Microbiota
MH  - *Flame Retardants
OTO - NOTNLM
OT  - Biotransformation
OT  - Dehalogenase
OT  - HBCDs
OT  - Microbial community
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/03/18 00:42
MHDA- 2024/04/08 06:42
CRDT- 2024/03/17 19:08
PHST- 2023/11/15 00:00 [received]
PHST- 2024/02/29 00:00 [revised]
PHST- 2024/03/12 00:00 [accepted]
PHST- 2024/04/08 06:42 [medline]
PHST- 2024/03/18 00:42 [pubmed]
PHST- 2024/03/17 19:08 [entrez]
AID - S0304-3894(24)00615-0 [pii]
AID - 10.1016/j.jhazmat.2024.134036 [doi]
PST - ppublish
SO  - J Hazard Mater. 2024 May 5;469:134036. doi: 10.1016/j.jhazmat.2024.134036. Epub 
      2024 Mar 13.