PMID- 38496427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
DP  - 2024 Mar 5
TI  - Salmonella Typhimurium infection inhibits macrophage IFNbeta signaling in a 
      TLR4-dependent manner.
LID - 2024.03.05.583530 [pii]
LID - 10.1101/2024.03.05.583530 [doi]
AB  - Type I Interferons (IFNs) generally have a protective role during viral 
      infections, but their function during bacterial infections is dependent on the 
      bacterial species. Legionella pneumophila, Shigella sonnei and Mycobacterium 
      tuberculosis can inhibit type I IFN signaling. Here we examined the role of type 
      I IFN, specifically IFNbeta, in the context of Salmonella enterica serovar 
      Typhimurium (STm) macrophage infections and the capacity of STm to inhibit type I 
      IFN signaling. We demonstrate that IFNbeta has no effect on the intracellular growth 
      of STm in infected bone marrow derived macrophages (BMDMs) derived from C57BL/6 
      mice. STm infection inhibits IFNbeta signaling but not IFNgamma signaling in a murine 
      macrophage cell line. We show that this inhibition is independent of the type III 
      and type VI secretion systems expressed by STm and is also independent of 
      bacterial phagocytosis. The inhibition is Toll-like receptor 4 (TLR4)-dependent 
      as the TLR4 ligand, lipopolysaccharide (LPS), alone is sufficient to inhibit 
      IFNbeta-mediated signaling and STm-infected, TLR4-deficient BMDMs do not exhibit 
      inhibited IFNbeta signaling. In summary, we show that macrophages exposed to STm 
      have reduced IFNbeta signaling via crosstalk with TLR4 signaling, and that IFNbeta 
      signaling does not affect cell autonomous host defense against STm.
FAU - Shuster, Michael
AU  - Shuster M
AUID- ORCID: 0000-0002-6861-8398
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Lyu, Zhihui
AU  - Lyu Z
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Augenstreich, Jacques
AU  - Augenstreich J
AUID- ORCID: 0000-0003-2147-2675
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Mathur, Shrestha
AU  - Mathur S
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Ganesh, Akshaya
AU  - Ganesh A
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Ling, Jiqiang
AU  - Ling J
AUID- ORCID: 0000-0003-4466-8304
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
FAU - Briken, Volker
AU  - Briken V
AUID- ORCID: 0000-0001-5830-6107
AD  - Department of Cell Biology and Molecular Genetics, University of Maryland, 
      College Park, MD, USA.
LA  - eng
GR  - R21 AI142396/AI/NIAID NIH HHS/United States
GR  - R01 AI139492/AI/NIAID NIH HHS/United States
GR  - R35 GM136213/GM/NIGMS NIH HHS/United States
GR  - T32 GM080201/GM/NIGMS NIH HHS/United States
GR  - S10 OD025223/OD/NIH HHS/United States
PT  - Preprint
DEP - 20240305
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10942315
EDAT- 2024/03/18 06:42
MHDA- 2024/03/18 06:43
PMCR- 2024/03/15
CRDT- 2024/03/18 04:37
PHST- 2024/03/18 06:42 [pubmed]
PHST- 2024/03/18 06:43 [medline]
PHST- 2024/03/18 04:37 [entrez]
PHST- 2024/03/15 00:00 [pmc-release]
AID - 2024.03.05.583530 [pii]
AID - 10.1101/2024.03.05.583530 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2024 Mar 5:2024.03.05.583530. doi: 10.1101/2024.03.05.583530.