PMID- 38499507
OWN - NLM
STAT- MEDLINE
DCOM- 20240416
LR  - 20240416
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 48
IP  - 5
DP  - 2024 May
TI  - Silver nanoparticles stimulate 5-Fluorouracil-induced colorectal cancer cells to 
      kill through the upregulation TRPV1-mediated calcium signaling pathways.
PG  - 712-725
LID - 10.1002/cbin.12141 [doi]
AB  - The involvement of the TRP vanilloid 1 (TRPV1) cation channel on the 
      5-Fluorouracil (5-FU)-caused Ca(2+) signals through the activation of the 
      apoptotic signaling pathway and stimulating the mitochondrial Ca(2+) and Zn(2+) 
      accumulation-induced reactive oxygen species (ROS) productions in several cancer 
      cells, except the colorectal cancer (HT-29) cell line, was recently reported. I 
      aimed to investigate the action of silver nanoparticles (SiNPs) and 5-FU 
      incubations through the activation of TRPV1 on ROS, apoptosis, and cell death in 
      the HT-29 cell line. The cells were divided into four groups: control, SiNP 
      (100 microM for 48 h), 5-FU (25 muM for 24 h), and 5-FU + SiNP. SiNP treatment through 
      TRPV1 activation (via capsaicin) stimulated the oxidant and apoptotic actions of 
      5-FU in the cells, whereas they were diminished in the cells by the TRPV1 
      antagonist (capsazepine) treatment. The apoptotic and cell death actions of 5-FU 
      were determined by increasing the propidium iodide/Hoechst rate, caspase-3, -8, 
      and -9 activations, mitochondrial membrane depolarization, lipid peroxidation, 
      and ROS, but decreasing the glutathione and glutathione peroxidase. The increase 
      of cytosolic free Ca(2+) and Zn(2+) into mitochondria via the stimulation of 
      TRPV1 current density increased oxidant and apoptotic properties of 5-FU in the 
      cells. For the therapy of HT-29 tumor cells, I found that the combination of 
      SiNPs and 5-FU was synergistic via TRPV1 activation.
CI  - (c) 2024 International Federation of Cell Biology.
FAU - Kaya, Muge Mavioglu
AU  - Kaya MM
AUID- ORCID: 0000-0003-1276-3745
AD  - Department of Molecular Biology and Genetics, Faculty of Science, Kafkas 
      University, Kars, Turkiye.
LA  - eng
GR  - 2022-03/BSN Health, Analysis and Innovation Ltd. Goller Bolgesi Teknokenti, 
      Isparta, Turkiye/
PT  - Journal Article
DEP - 20240318
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Reactive Oxygen Species)
RN  - U3P01618RT (Fluorouracil)
RN  - 3M4G523W1G (Silver)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Oxidants)
RN  - SY7Q814VUP (Calcium)
RN  - 0 (TRPV1 protein, human)
RN  - 0 (TRPV Cation Channels)
SB  - IM
MH  - Humans
MH  - Reactive Oxygen Species/metabolism
MH  - Fluorouracil/pharmacology
MH  - Oxidative Stress
MH  - Silver/pharmacology
MH  - Calcium Signaling
MH  - *Metal Nanoparticles
MH  - Up-Regulation
MH  - *Antineoplastic Agents/pharmacology
MH  - Apoptosis
MH  - Oxidants/pharmacology
MH  - *Colorectal Neoplasms/drug therapy
MH  - Calcium/metabolism
MH  - TRPV Cation Channels/metabolism
OTO - NOTNLM
OT  - 5-Fluorouracil
OT  - TRPV1 channel
OT  - apoptosis
OT  - colorectal cancer
OT  - mitochondria
OT  - silver nanoparticles
EDAT- 2024/03/19 06:43
MHDA- 2024/04/16 12:43
CRDT- 2024/03/19 00:03
PHST- 2024/01/25 00:00 [revised]
PHST- 2023/10/05 00:00 [received]
PHST- 2024/02/05 00:00 [accepted]
PHST- 2024/04/16 12:43 [medline]
PHST- 2024/03/19 06:43 [pubmed]
PHST- 2024/03/19 00:03 [entrez]
AID - 10.1002/cbin.12141 [doi]
PST - ppublish
SO  - Cell Biol Int. 2024 May;48(5):712-725. doi: 10.1002/cbin.12141. Epub 2024 Mar 18.