PMID- 38499964
OWN - NLM
STAT- Publisher
LR  - 20240319
IS  - 1573-4927 (Electronic)
IS  - 0006-2928 (Linking)
DP  - 2024 Mar 18
TI  - Molecular Characterization and Seasonal Variation in Expression of HSP70.1 Gene 
      in Gangatiri Cattle and Its Comparison with Buffalo.
LID - 10.1007/s10528-024-10739-z [doi]
AB  - Under tropical climate heat stress is a major challenge for livestock production. 
      HSP70.1 is a ubiquitously expressed protein maintaining cellular machinery 
      through proper folding of denatured proteins and prevents cellular apoptosis and 
      protect cell from heat stress. Therefore, present investigation was undertaken to 
      explore genetic variability in HSP70.1 gene in Gangatiri cattle, its comparison 
      with buffalo sequences and differential expression in different season. The 
      allelic variant was identified by sequencing amplified PCR product of HSP70.1 
      gene by primer walking. Season-wise total RNA samples was prepared for 
      differential expression study. Brilliant SYBR Green QPCR technique was used to 
      study the expression kinetics of this gene. DNA sequencing by primer walking 
      identified four allelic variants in Gangatiri cattle. Sequence alignment study 
      revealed four, six and one substitutions in the 5' untranslated region (5'UTR), 
      coding and 3' untranslated region ((3'UTR) of HSP70.1 gene, respectively. 
      Comparative analysis of HSP70.1 gene revealed that Cattle has shorter 5'UTR and 
      3' UTR than the buffalo. In Gangatiri cattle, summer season has significantly 
      higher (P </= 0.05) expression of HSP70.1 than the spring and winter. The relative 
      expression of HSP70.1 was increased by more than six folds in summer and nearly 
      1.5 folds higher in winter in comparison to the spring season. Therefore, HSP70.1 
      may be considered to have a critical role in the development of thermal tolerance 
      in Gangatiri cattle.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Dayal, Shanker
AU  - Dayal S
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India. antudayal@gmail.com.
FAU - Kumar, Birendra
AU  - Kumar B
AD  - Department of Animal Genetics and Breeding, Bihar Veterinary College, Patna, 
      Bihar, 800014, India.
FAU - Kumari, Rajni
AU  - Kumari R
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India.
FAU - Kumar, Jyoti
AU  - Kumar J
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India.
FAU - Ray, Pradeep Kumar
AU  - Ray PK
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India.
FAU - Chandran, P C
AU  - Chandran PC
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India.
FAU - Dey, Amitava
AU  - Dey A
AD  - Division of Livestock and Fishery Management, ICAR Research Complex for Eastern 
      Region, Patna, Bihar, 800014, India.
LA  - eng
GR  - ICAR-RCER/DLFM/2014/14l/ICAR Research Complex for Eastern region/
PT  - Journal Article
DEP - 20240318
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
SB  - IM
OTO - NOTNLM
OT  - Allelic variants
OT  - Differential expression
OT  - Gangatiri cattle
OT  - HSP70.1
OT  - Heat stress
EDAT- 2024/03/19 06:43
MHDA- 2024/03/19 06:43
CRDT- 2024/03/19 00:40
PHST- 2023/06/20 00:00 [received]
PHST- 2024/02/12 00:00 [accepted]
PHST- 2024/03/19 06:43 [medline]
PHST- 2024/03/19 06:43 [pubmed]
PHST- 2024/03/19 00:40 [entrez]
AID - 10.1007/s10528-024-10739-z [pii]
AID - 10.1007/s10528-024-10739-z [doi]
PST - aheadofprint
SO  - Biochem Genet. 2024 Mar 18. doi: 10.1007/s10528-024-10739-z.