PMID- 38501171
OWN - NLM
STAT- Publisher
LR  - 20240319
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
DP  - 2024 Mar 19
TI  - DP7-C/mir-26a system promotes bone regeneration by remodeling the osteogenic 
      immune microenvironment.
LID - 10.1111/odi.14910 [doi]
AB  - OBJECTIVE: This study investigates the DP7-C/miR-26a complex as a stable entity 
      resulting from the combination of miR-26a with the immunomodulatory peptide 
      DP7-C. Our focus is on utilizing DP7-C loaded with miR-26a to modulate the immune 
      microenvironment in bone and facilitate osteogenesis. METHODS: The DP7-C/miR-26a 
      complex was characterized through transmission electron microscopy, agarose 
      electrophoresis, and nanoparticle size potentiometer analysis. Transfection 
      efficiency and cytotoxicity of DP7-C were assessed using flow cytometry and the 
      CCK-8 assay. We validated the effects of DP7-C/miR-26a on bone marrow mesenchymal 
      stem cells (BMSCs) and macrophages RAW 264.7 through gene expression and protein 
      synthesis assays. A comprehensive evaluation of appositional bone formation 
      involved micro-CT imaging, histologic analysis, and immunohistochemical staining. 
      RESULTS: DP7-C/miR-26a, a nanoscale, and low-toxic cationic complex, demonstrated 
      the ability to enter BMSCs and RAW 264.7 via distinct pathways. The treatment 
      with DP7-C/miR-26a significantly increased the synthesis of multiple 
      osteogenesis-related factors in BMSCs, facilitating calcium nodule formation 
      in vitro. Furthermore, DP7-C/miR-26a promoted M1 macrophage polarization toward 
      M2 while suppressing the release of inflammatory factors. Coculture studies 
      corroborated these findings, indicating significant repair of rat skull defects 
      following treatment with DP7-C/miR-26a. CONCLUSION: The DP7-C/miR-26a system 
      offers a safer, more efficient, and feasible technical means for treating bone 
      defects.
CI  - (c) 2024 Wiley Periodicals LLC.
FAU - Huang, Jie
AU  - Huang J
AUID- ORCID: 0000-0001-9976-2301
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
AD  - General Dentistry, Chongqing University Three Gorges Hospital, Chongqing, China.
FAU - Yang, Yiling
AU  - Yang Y
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
FAU - Zhu, Yushu
AU  - Zhu Y
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
FAU - Xiao, Xun
AU  - Xiao X
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
FAU - Yalikun, Kaidiliya
AU  - Yalikun K
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
FAU - Jiang, Xiliang
AU  - Jiang X
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
FAU - Yang, Li
AU  - Yang L
AD  - State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation 
      Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
FAU - Mu, Yandong
AU  - Mu Y
AUID- ORCID: 0000-0002-1829-6116
AD  - Department of Stomatology, Sichuan Provincial People's Hospital, University of 
      Electronic Science and Technology of China, Chengdu, China.
LA  - eng
GR  - National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20240319
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
OTO - NOTNLM
OT  - DP7-C
OT  - immunomodulatory
OT  - immunomodulatory peptide
OT  - miR-26a
OT  - osteogenesis
EDAT- 2024/03/19 06:44
MHDA- 2024/03/19 06:44
CRDT- 2024/03/19 04:02
PHST- 2024/02/14 00:00 [revised]
PHST- 2023/08/10 00:00 [received]
PHST- 2024/02/19 00:00 [accepted]
PHST- 2024/03/19 06:44 [medline]
PHST- 2024/03/19 06:44 [pubmed]
PHST- 2024/03/19 04:02 [entrez]
AID - 10.1111/odi.14910 [doi]
PST - aheadofprint
SO  - Oral Dis. 2024 Mar 19. doi: 10.1111/odi.14910.