PMID- 38502479
OWN - NLM
STAT- MEDLINE
DCOM- 20240321
LR  - 20240321
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2784
DP  - 2024
TI  - SABER-FISH in Hydractinia.
PG  - 77-85
LID - 10.1007/978-1-0716-3766-1_5 [doi]
AB  - In situ hybridization allows the detection of nucleic acid sequences in fixed 
      cells and tissues. The gelatinous nature of cnidarians and Hydractinia demands 
      extensive and exhausting protocols to detect RNA transcripts with traditional 
      methods (e.g., colorimetric in situ hybridization). Signal amplification by 
      exchange reaction (SABER) fluorescence in situ hybridization (FISH) enables 
      simplifying and multiplex imaging of RNA targets in a rapid and cost-effective 
      manner. In one enzymatic reaction, SABER-FISH uses a strand-displacing polymerase 
      and catalytic DNA hairpin to generate FISH probes with adjustable signal 
      amplification, allowing highly sensitive detection of nucleic acids and reducing 
      the number of required probes. Here I describe the methodology to detect 
      transcripts within the cells of Hydractinia by SABER-FISH in whole-mount samples.
CI  - (c) 2024. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Salinas-Saavedra, Miguel
AU  - Salinas-Saavedra M
AD  - University of Galway, Galway, Ireland.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 63231-63-0 (RNA)
RN  - 0 (Nucleic Acids)
RN  - Hydractinia
SB  - IM
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *RNA
MH  - *Nucleic Acids
MH  - *Hydrozoa
OTO - NOTNLM
OT  - Cnidaria
OT  - Hydractinia
OT  - In situ hybridization
OT  - RNA
OT  - SABER-FISH
EDAT- 2024/03/19 18:42
MHDA- 2024/03/21 12:48
CRDT- 2024/03/19 12:21
PHST- 2024/03/21 12:48 [medline]
PHST- 2024/03/19 18:42 [pubmed]
PHST- 2024/03/19 12:21 [entrez]
AID - 10.1007/978-1-0716-3766-1_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2024;2784:77-85. doi: 10.1007/978-1-0716-3766-1_5.