PMID- 38503705
OWN - NLM
STAT- MEDLINE
DCOM- 20240321
LR  - 20240322
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 42
IP  - 3
DP  - 2024 Mar 8
TI  - [Determination of 35 prohibited pesticide residues in Saussurea costus by 
      modified QuEChERS method based on multi-walled carbon nanotubes coupled with gas 
      chromatography-tandem mass spectrometry].
PG  - 282-290
LID - 10.3724/SP.J.1123.2023.07018 [doi]
AB  - Saussurea costus, a perennial herb belonging to the Asteraceae family, is a vital 
      ingredient in traditional Chinese medicine. Increased demands for the herb have 
      led to its widespread cultivation in China, but the corresponding increase in 
      pesticide use has raised concerns about pesticide residues. Such residues would 
      affect the safety and global market potential of Saussurea costus. Thus, a simple 
      method is crucial to detect pesticide residues. The QuEChERS technique, in 
      combination with gas chromatography-tandem mass spectrometry (GC-MS/MS), is 
      commonly used for residue detection. However, traditional adsorbents may be 
      unable to purify complex herbal mixtures well, affecting accuracy and instrument 
      performance. Choosing suitable purification materials for Saussurea costus 
      samples with complex matrices is of significant importance. This study focused on 
      the detection of 35 prohibited pesticides in Saussurea costus. A rapid detection 
      method was established by combining the QuEChERS technique with GC-MS/MS and 
      utilizing a combination of multiwalled carbon nanotubes (MWCNTs), 
      octadecylsilane-bonded silica gel (C(18)), and anhydrous magnesium sulfate 
      (MgSO(4)) as the purification adsorbent. The samples were extracted with 
      acetonitrile, purified by an improved QuEChERS process, subjected to GC-MS/MS 
      analysis in multiple reaction monitoring (MRM) mode, and quantified using the 
      internal standard method. The purification effects of four materials (C(18), 
      MWCNTs, N-propyl ethylenediamine (PSA), and graphitized carbon black (GCB)) and 
      their optimal dosages were investigated by considering the matrix characteristics 
      of the samples. An orthogonal experimental design was employed to optimize the 
      ratio of adsorbent combinations, and the optimal adsorbent combination was 
      determined to be 450 mg of MgSO(4), 400 mg of C(18), and 50 mg of MWCNTs. Matrix 
      effect (ME) evaluation of the S. costus matrix showed that 31 target compounds 
      strongly exhibited matrix-enhancement effects. Thus, matrix-matched calibration 
      was employed in this study. Methodological investigation revealed that the 
      standard curves for the 35 pesticides exhibited good linearity, with correlation 
      coefficients (r(2)) greater than 0.9970. The average recoveries at three spiked 
      levels ranged from 69.6% to 126.9%, and the relative standard deviations (RSDs) 
      for parallel groups were all less than 10%. The limits of detection (LODs) and 
      quantification (LOQs) ranged from 0.2 to 5.4 mug/kg and from 0.6 to 18.1 mug/kg, 
      respectively. The developed method was used to screen and detect 35 pesticide 
      residues in 20 batches of S. costus samples, and the target compounds were 
      detected in six batches. The proposed method is simple, sensitive, and accurate. 
      Thus, it is suitable for the rapid screening and detection of the 35 pesticide 
      residues in S. costus and provides technical support for the cultivation, 
      production, and quality control of the herb.
FAU - Yi, Cong-Sheng
AU  - Yi CS
AD  - Chinese Academy of Sciences Testing Technical Services (Guangzhou) Co., Ltd., 
      Guangzhou 510650, China.
FAU - Liu, Rui
AU  - Liu R
AD  - Chinese Academy of Sciences Testing Technical Services (Guangzhou) Co., Ltd., 
      Guangzhou 510650, China.
FAU - Wu, Zhi-Peng
AU  - Wu ZP
AD  - Chinese Academy of Sciences Testing Technical Services (Guangzhou) Co., Ltd., 
      Guangzhou 510650, China.
FAU - Liu, Bo
AU  - Liu B
AD  - Chinese Academy of Sciences Testing Technical Services (Guangzhou) Co., Ltd., 
      Guangzhou 510650, China.
FAU - DU, Lei
AU  - DU L
AD  - Chinese Academy of Sciences Testing Technical Services (Guangzhou) Co., Ltd., 
      Guangzhou 510650, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Pesticide Residues)
SB  - IM
MH  - Tandem Mass Spectrometry/methods
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - *Nanotubes, Carbon/analysis/chemistry
MH  - *Pesticide Residues/analysis
MH  - *Saussurea
PMC - PMC10951808
OTO - NOTNLM
OT  - QuEChERS
OT  - Saussurea costus
OT  - gas chromatography-tandem mass spectrometry (GC-MS/MS)
OT  - multi-walled carbon nanotubes
OT  - prohibited pesticides
EDAT- 2024/03/20 00:42
MHDA- 2024/03/21 12:46
PMCR- 2024/03/08
CRDT- 2024/03/19 23:23
PHST- 2024/03/21 12:46 [medline]
PHST- 2024/03/20 00:42 [pubmed]
PHST- 2024/03/19 23:23 [entrez]
PHST- 2024/03/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2023.07018 [doi]
PST - ppublish
SO  - Se Pu. 2024 Mar 8;42(3):282-290. doi: 10.3724/SP.J.1123.2023.07018.