PMID- 38507005
OWN - NLM
STAT- MEDLINE
DCOM- 20240322
LR  - 20240322
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2789
DP  - 2024
TI  - In Vitro and In Vivo Methods for Analysis of Nanoparticles' Potential to Induce 
      Delayed-Type Hypersensitivity Reactions.
PG  - 193-207
LID - 10.1007/978-1-0716-3786-9_20 [doi]
AB  - Delayed-type hypersensitivity (DTH) reactions are among the common reasons for 
      drug withdrawal from clinical use during the post-marketing stage. Several in 
      vivo methods have been developed to test DTH responses in animal models. They 
      include the local lymph node assay (LLNA) and local lymph node proliferation 
      assay (LLNP). While LLNA is instrumental in testing topically administered 
      formulations (e.g., creams), the LLNP was proven to be predictive of 
      drug-mediated DTH in response to small molecule pharmaceuticals. Global efforts 
      in reducing the use of research animals lead to the development of in vitro 
      models to predict test-materials' mediated DTH. Two such models include 
      the analysis of surface marker expression in human cell lines THP-1 and U-937. 
      These tests are known as the human cell line activation test (hCLAT) and myeloid 
      U937 skin sensitization test (MUSST or U-SENS), respectively. Here we describe 
      experimental procedures for all these methods, discuss their in vitro-in vivo 
      correlation, and suggest a strategy for applying these tests to analyze 
      engineered nanomaterials and nanotechnology-formulated drug products.
CI  - (c) 2024. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Potter, Timothy M
AU  - Potter TM
AD  - Nanotechnology Characterization Laboratory, Cancer Research Technology Program, 
      Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
FAU - Neun, Barry W
AU  - Neun BW
AD  - Nanotechnology Characterization Laboratory, Cancer Research Technology Program, 
      Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
FAU - Dobrovolskaia, Marina A
AU  - Dobrovolskaia MA
AD  - Nanotechnology Characterization Laboratory, Cancer Research Technology Program, 
      Frederick National Laboratory for Cancer Research, Frederick, MD, USA. 
      marina@mail.nih.gov.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Allergens)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Skin Tests/methods
MH  - *Local Lymph Node Assay
MH  - Allergens
MH  - Cell Line
MH  - *Nanoparticles/toxicity
OTO - NOTNLM
OT  - Allergen
OT  - Hypersensitivity
OT  - Irritant
OT  - LLNA
OT  - LLNP
OT  - Leukocyte proliferation
OT  - MUSST
OT  - Nanoparticles
OT  - Sensitizer
OT  - hCLAT
EDAT- 2024/03/20 18:46
MHDA- 2024/03/22 06:44
CRDT- 2024/03/20 12:07
PHST- 2024/03/22 06:44 [medline]
PHST- 2024/03/20 18:46 [pubmed]
PHST- 2024/03/20 12:07 [entrez]
AID - 10.1007/978-1-0716-3786-9_20 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2024;2789:193-207. doi: 10.1007/978-1-0716-3786-9_20.